Q5VKP6 (NCAP_IRKV) Reviewed, UniProtKB/Swiss-Prot
Last modified March 6, 2013. Version 30. History...
Names and origin
|Protein names||Recommended name:|
Short name=Protein N
|Organism||Irkut virus (IRKV) [Complete proteome]|
|Taxonomic identifier||249583 [NCBI]|
|Taxonomic lineage||Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus|
|Virus host||Murina leucogaster (Greater tube-nosed bat) [TaxID: 187017]|
|Sequence length||451 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases By similarity.
Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, binds protein P and thereby positions the polymerase on the template. Protein P acts as a chaperone on free protein N to prevent it from aggregation before encapsidating genomic RNA By similarity.
Phosphorylated by host CK2. Unphosphorylated protein N seems to have a better affinity for leader viral promoter encapsidation. Phosphorylation of protein N in ribonucleocapsid may stabilize the interaction with protein P, thereby playing an important role in viral transcription/replication By similarity.
Displays a superantigen activity in human and mouse, activating mostly V-beta-8 subtypes of T-cell receptor By similarity.
Belongs to the lyssavirus nucleocapsid protein family.
|Cellular component||Host cytoplasm|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Cellular_component||host cell cytoplasm|
Inferred from electronic annotation. Source: UniProtKB-SubCellribonucleoprotein complex
Inferred from electronic annotation. Source: UniProtKB-KWviral nucleocapsid
Inferred from electronic annotation. Source: InterPro
Inferred from electronic annotation. Source: UniProtKB-KW
|Complete GO annotation...|
Sequence annotation (Features)
|||"Phylogenetic relationships of Irkut and West Caucasian bat viruses within the Lyssavirus genus and suggested quantitative criteria based on the N gene sequence for lyssavirus genotype definition."|
Kuzmin I.V., Hughes G.J., Botvinkin A.D., Orciari L.A., Rupprecht C.E.
Virus Res. 111:28-43(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"Complete genomes of Aravan, Khujand, Irkut and West Caucasian bat viruses, with special attention to the polymerase gene and non-coding regions."|
Kuzmin I.V., Wu X., Tordo N., Rupprecht C.E.
Virus Res. 136:81-90(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
|EF614260 mRNA. Translation: AAR03477.1.|
3D structure databases
|SMR||Q5VKP6. Positions 5-448. |
Protocols and materials databases
Family and domain databases
|Gene3D||1.10.3570.10. 1 hit. |
1.10.3610.10. 1 hit.
|InterPro||IPR000448. Rhabdo_ncapsid. |
|Pfam||PF00945. Rhabdo_ncap. 1 hit. |
|ProDom||PD002087. Rhabd_nucleocap. 1 hit. |
[Graphical view] [Entries sharing at least one domain]
|SUPFAM||SSF140809. SSF140809. 1 hit. |
|Accession||Primary (citable) accession number: Q5VKP6|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families