Q5VJL3 (GDT9_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase gdt9
EC=2.7.11.1

Alternative name(s):
Growth-differentiation transition protein 9
Protein kinase Dusty

Gene names

Name:	gdt9
ORF Names:	DDB_G0278879

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	In the N-terminal section; belongs to the GDT family. In the C-terminal section; belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular space Inferred from direct assay PubMed 20422638. Source: dictyBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 1577	1561	Probable serine/threonine-protein kinase gdt9		PRO_0000323586
Regions
	Topological domain	17 – 966	950	Extracellular Potential
	Transmembrane	967 – 987	21	Helical; Potential
	Topological domain	988 – 1577	590	Cytoplasmic Potential
	Domain	1290 – 1573	284	Protein kinase
	Nucleotide binding	1296 – 1304	9	ATP By similarity
	Compositional bias	649 – 668	20	Thr-rich
	Compositional bias	762 – 767	6	Poly-Ser
	Compositional bias	776 – 779	4	Poly-Thr
	Compositional bias	931 – 947	17	Ser-rich
	Compositional bias	1091 – 1115	25	Asn-rich
Sites
	Active site	1436	1	Proton acceptor By similarity
	Binding site	1317	1	ATP By similarity
Experimental info
	Sequence conflict	283	1	N → K in AAS55398. Ref.1
	Sequence conflict	283	1	N → K in AAS55399. Ref.1
	Sequence conflict	1274 – 1275	2	CV → WG in AAS55398. Ref.1
	Sequence conflict	1274 – 1275	2	CV → WG in AAS55399. Ref.1
	Sequence conflict	1552	1	I → R in AAS55398. Ref.1
	Sequence conflict	1552	1	I → R in AAS55399. Ref.1
	Sequence conflict	1559	1	L → R in AAS55398. Ref.1
	Sequence conflict	1559	1	L → R in AAS55399. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5VJL3 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 8473361205CCE7ED	FASTA	1,577	175,900
	10 20 30 40 50 60 MKTFLLIFLL ICVCKGITNI TTPSIYFDLK KINRFQDYLN NGITPSINLT FHDFYYPDVC 70 80 90 100 110 120 VNTIKNIDGS TNFVKSSFLN APTNFANLYI TKGSKMTVKP NQILGAFINI LCIEGSLEVP 130 140 150 160 170 180 VGVFPFFIGA LVILPGGNFK SESSFRFMNL VNSKIDPLNF FPGILTLGGS LSILGPKSIK 190 200 210 220 230 240 YSANRLGDFQ FQLLELIDPN SFNSDYKANI YSESFVTGQQ CDFVSITNGY TLTVGGCQGV 250 260 270 280 290 300 PSTDVNIYIF FATVDYDCTF IDSDSEVPAS IYISGDTNVH IENFFLNSLG KTTNANYNDT 310 320 330 340 350 360 ILIFSPTDDT NVTDIIIGTN QRYRNSLFVE FSNNVTLRNN FIRESIESRS PLMFFASSPI 370 380 390 400 410 420 LEGNLVVSNS GSSVIAQFGT EFIQSTNNSY FLEPPTQPVI PNDNNMDYGT EGNGIYSLSP 430 440 450 460 470 480 IVSFTTDVFV GQLISINFNF IANRSLVTGF NYDCFAPCYP GSPIISNLVQ HSVDFNIIKP 490 500 510 520 530 540 TFSYSHGNTT SSTTTSSTTT NTNSHFLLNV NDNGNEPSSY YSFNQLSIAS EAIKVNLPIG 550 560 570 580 590 600 TLGIGNLFAT NNFKIDGALE RLDILNSIFN LPPPISNNFS QSGILLSTTT SMLNSYVFVS 610 620 630 640 650 660 IGSEPTRIES QIYGSTITPY YYNNIETLDL FQIQSIFPSV QYQIIKNSTI NITINITTTT 670 680 690 700 710 720 TTTTTPTTNN VVCSFTSIVN QQSSVTNEIS INETLIQMDP ILNNCVFPLS IDQEGSLKLR 730 740 750 760 770 780 VTIKNQNSTL ENQYYYIIDF PEITIFQSFQ FYTGIKFIDV QSSSSSSQQT SSFSPTTTTF 790 800 810 820 830 840 LNLLSTTENS NGFLNGCQIS NQCTLSNNVK YVSSLPNVTN SPDFQLFQSG ITPIIPYDPV 850 860 870 880 890 900 VINLGIEKNK TFQFQLFFTF YQPIDQYSSP LSIFIQSEPY LLLDPLIDAP NFKNFTFFYD 910 920 930 940 950 960 NLNQDSIEIS FISRGDIYLT SMAIYSLDLV SYESSDSSSS SSNSSSSTGN GIGDIVNNED 970 980 990 1000 1010 1020 NHKKLVIALS VSIPVAALLV ILCFGIFICY NNNKKNKNET KGKDIETNTD KKDDENENEN 1030 1040 1050 1060 1070 1080 CKFQFKDEFL TNPNEINIQL KKLMINKSST LPPQSTISID TSPSSENTTF TESLTPKKSA 1090 1100 1110 1120 1130 1140 TVNGEIDFSR NSTNESTVSN SSSENNSDNN NNNNNKCKKE KLSSFPTIPG TNLTNIPLNL 1150 1160 1170 1180 1190 1200 VGRKSRNPED KYRTNNDILV CLQESHYFKP DDPSIPIEFS ESVLDFGRGS KCLIDETYIY 1210 1220 1230 1240 1250 1260 TISVKNKSTT DYNILLILPI DNNTGTISTD TQYFQINAGE SKPISFSISL NCTTKYFEKI 1270 1280 1290 1300 1310 1320 GVSIEELNYH TFICVHMESE LSTRLDFSEL DFDEICGQGT YGMVYKGKWR SQVVAIKMMR 1330 1340 1350 1360 1370 1380 VGTVNCDEVY REMDIMGKIR HQNVISFIGC VVSLEHLCIV TEFSPIGSLG DLIHGDNNQK 1390 1400 1410 1420 1430 1440 KSNTNTITNT ITNTNTNTNT STSTSTKLTV KQKLRIAIDI ARGCQFLHQC GIIHRDLKPD 1450 1460 1470 1480 1490 1500 NVLVFDINHN APVCAKISDF GTSKETNEFS NKNTNCVGTP IYMSNEILEK KTYDNSTDVY 1510 1520 1530 1540 1550 1560 SFAVLFYEMM IEEVPFSEID EKWEIPSKVI SGWRPTKGLN SLDRDIKNLI NICWAPHSLP 1570 SFDEIVFSLV KIFKRFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of slime mold dusty protein kinase gene." Huang C.-H. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY429682 mRNA. Translation: AAS55398.1. AY429683 Genomic DNA. Translation: AAS55399.1. AAFI02000024 Genomic DNA. Translation: EAS66889.1.
RefSeq	XP_001134573.1. XM_001134573.1.
3D structure databases
ProteinModelPortal	Q5VJL3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0232936; DDB0232936; DDB_G0278879.
GeneID	8621755.
KEGG	ddi:DDB_G0278879.
Organism-specific databases
dictyBase	DDB_G0278879. gdt9.
Phylogenomic databases
eggNOG	COG0515.
OMA	CHKEEND.
ProtClustDB	CLSZ2847455.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. IPR026237. STKINASEGDT. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR02079. STKINASEGDT.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GDT9_DICDI

Accession

Primary (citable) accession number: Q5VJL3
Secondary accession number(s): Q1ZXI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)