Reviewed, UniProtKB/Swiss-Prot Q5V9F0 (AMPL_DICDI)

Names and origin

Protein names

Recommended name:
    Cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
    Leucyl aminopeptidase
      Short name=LAP
    Proline aminopeptidase
    EC=3.4.11.5
    Prolyl aminopeptidase

Gene names

Name:	lap
ORF Names:	DDB_G0279793

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. Release of N-terminal proline from a peptide.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Cytosol aminopeptidase		PRO_0000328614
Sites
	Active site	298	1	By similarity
	Active site	372	1	By similarity
	Metal binding	286	1	Zinc 2 By similarity
	Metal binding	291	1	Zinc 1 By similarity
	Metal binding	291	1	Zinc 2 By similarity
	Metal binding	309	1	Zinc 2 By similarity
	Metal binding	368	1	Zinc 1 By similarity
	Metal binding	370	1	Zinc 1 By similarity
	Metal binding	370	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5V9F0-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: EE9ECBB8B54C0E85	FASTA	520	56,410
	10 20 30 40 50 60 MLKNIINKKN LFINNQQNIF QIIKRNKMTD STVDNKGYIV GIYENEEFTP LGQQLNEKTN 70 80 90 100 110 120 GHLLKSIKLS DTKGKVGDNL VLYNVTPEVS RVAIVGLGKK ENNNSTTYEK NENTRKAIGS 130 140 150 160 170 180 GVKALKSKNA THLTIDSNIG DAKQTAEGAF LSNFKFDFKT GTSGKTANST NESIQVQLSP 190 200 210 220 230 240 SPSSEECFKE GKILAESQNF ARVLMETPAN LLTPTNFVQH VSSQMKELID SGKVEMIVRE 250 260 270 280 290 300 EQWVKDQKMG MFWGVAKGSD EPLKFLELHY RGASADGKDS IVYVGKGITF DSGGISIKPS 310 320 330 340 350 360 ANMGLMKGDM GGAATAVSAM FGVASLGLKV NLITITPLCE NMPSGKATKP GDILTAANGK 370 380 390 400 410 420 TVEVDNTDAE GRLILGDALH YACSFKPTHI IDIATLTGAI DVALGQHYAG CFTTTDSLWD 430 440 450 460 470 480 QLNECGNISG ERLWRMPLIP EYRKQMETSK VADLINSAGR SGGACCAAGF LKEFITADQS 490 500 510 520 WSHLDIAGVM SSSEDGPYIR KGMTGKPTRT LIEFAKKNQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Huang J., Mullapudi N., Lancto C., Scott M., Abrahamsen M., Kissinger J.C. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY581145 Genomic DNA. Translation: AAT92029.1. AAFI02000032 Genomic DNA. Translation: EAL67576.1.
RefSeq	XP_641537.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5V9F0.
Protein family/group databases
MEROPS	M17.001.
Genome annotation databases
GeneID	3392643.
GenomeReviews	Gene locus lap in contig CM000152_GR.
KEGG	ddi:DDB_0231024.
Organism-specific databases
dictyBase	DDB_G0279793. lap.
Phylogenomic databases
OMA	PPLLLEC.
Enzyme and pathway databases
BRENDA	3.4.11.1. 424. 3.4.11.5. 424.
Family and domain databases
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPL_DICDI

Accession

Primary (citable) accession number: Q5V9F0
Secondary accession number(s): Q54WC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	December 7, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)