ID   FTHS_HALMA              Reviewed;         553 AA.
AC   Q5V5Y2;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 2.
DT   08-NOV-2023, entry version 89.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=pNG7380;
OS   Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM
OS   B-1809) (Halobacterium marismortui).
OG   Plasmid pNG700.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Haloarculaceae; Haloarcula.
OX   NCBI_TaxID=272569;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809;
RX   PubMed=15520287; DOI=10.1101/gr.2700304;
RA   Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W.,
RA   Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E.,
RA   Hood L., Ng W.V.;
RT   "Genome sequence of Haloarcula marismortui: a halophilic archaeon from the
RT   Dead Sea.";
RL   Genome Res. 14:2221-2234(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAV45070.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AY596296; AAV45070.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q5V5Y2; -.
DR   SMR; Q5V5Y2; -.
DR   EnsemblBacteria; AAV45070; AAV45070; pNG7380.
DR   KEGG; hma:pNG7380; -.
DR   PATRIC; fig|272569.17.peg.800; -.
DR   HOGENOM; CLU_003601_3_3_2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001169; Plasmid pNG700.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism; Plasmid;
KW   Reference proteome.
FT   CHAIN           1..553
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199413"
FT   BINDING         56..63
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   553 AA;  59251 MW;  6FDA27CFF8595A76 CRC64;
     MEPIWELVEP WGLGLDDLQY FGEYTAKVKQ HAIERLREQA ENREQNLVLV TGMTPTPKGE
     GKTVTTVGLG QTLNHVGEEA MIAIREPSLG PVFGVKGGAA GGGRSQVLPM EDINLHFTGD
     LHALTSAHNL IAAMLDAKIS QGDDLNIDIN NVSWPRAIDM NDRALRETVV GLGGKTGGTP
     REDSFILTAA SELMAVLCLA SDIGDLKERV SRIIVAYDED GDPVTVEDIE ATGPATMLLR
     DAIKPNVVQT IEGTPALVHG GPFANIAHGT NSLVADKTAF GMGDYLVTEA GFGSDLGAEK
     FMDVVCRKGD MTPNAVVLVA SVRALKYHGL NQWPVDYDEI GEAGVEAVEA GFSNLDKHAR
     NLQKFGVPVV VSVNRFPDDT DEEVQAVLDH CREDLGVRAA ESNVFSDGSE GGVDLAENVI
     EATEESNEED FRMLYDDDDS IKEKIHTVAT EIYGADDVKY TGGALDDIEQ MNDLDFDDYP
     VVMSKTFHSL SDDASQKGAP EGWELEISEV YPSAGAGFLV ALTADALTMP GLPARPAAAD
     MDIDGDGNIS GLF
//