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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi56 – 638ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-3537-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:pNG7380
Encoded oniPlasmid pNG7000 Publication
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
ProteomesiUP000001169 Componenti: Plasmid pNG700

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Formate--tetrahydrofolate ligasePRO_0000199413Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5V5Y2.
SMRiQ5V5Y2. Positions 1-551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5V5Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPIWELVEP WGLGLDDLQY FGEYTAKVKQ HAIERLREQA ENREQNLVLV
60 70 80 90 100
TGMTPTPKGE GKTVTTVGLG QTLNHVGEEA MIAIREPSLG PVFGVKGGAA
110 120 130 140 150
GGGRSQVLPM EDINLHFTGD LHALTSAHNL IAAMLDAKIS QGDDLNIDIN
160 170 180 190 200
NVSWPRAIDM NDRALRETVV GLGGKTGGTP REDSFILTAA SELMAVLCLA
210 220 230 240 250
SDIGDLKERV SRIIVAYDED GDPVTVEDIE ATGPATMLLR DAIKPNVVQT
260 270 280 290 300
IEGTPALVHG GPFANIAHGT NSLVADKTAF GMGDYLVTEA GFGSDLGAEK
310 320 330 340 350
FMDVVCRKGD MTPNAVVLVA SVRALKYHGL NQWPVDYDEI GEAGVEAVEA
360 370 380 390 400
GFSNLDKHAR NLQKFGVPVV VSVNRFPDDT DEEVQAVLDH CREDLGVRAA
410 420 430 440 450
ESNVFSDGSE GGVDLAENVI EATEESNEED FRMLYDDDDS IKEKIHTVAT
460 470 480 490 500
EIYGADDVKY TGGALDDIEQ MNDLDFDDYP VVMSKTFHSL SDDASQKGAP
510 520 530 540 550
EGWELEISEV YPSAGAGFLV ALTADALTMP GLPARPAAAD MDIDGDGNIS

GLF
Length:553
Mass (Da):59,251
Last modified:December 6, 2005 - v2
Checksum:i6FDA27CFF8595A76
GO

Sequence cautioni

The sequence AAV45070.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY596296 Genomic DNA. Translation: AAV45070.1. Different initiation.
RefSeqiWP_011222756.1. NC_006395.1.
YP_134776.1. NC_006395.1.

Genome annotation databases

EnsemblBacteriaiAAV45070; AAV45070; pNG7380.
GeneIDi3127410.
KEGGihma:pNG7380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY596296 Genomic DNA. Translation: AAV45070.1. Different initiation.
RefSeqiWP_011222756.1. NC_006395.1.
YP_134776.1. NC_006395.1.

3D structure databases

ProteinModelPortaliQ5V5Y2.
SMRiQ5V5Y2. Positions 1-551.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV45070; AAV45070; pNG7380.
GeneIDi3127410.
KEGGihma:pNG7380.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciHMAR272569:GJDH-3537-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Entry informationi

Entry nameiFTHS_HALMA
AccessioniPrimary (citable) accession number: Q5V5Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 24, 2015
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.