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Q5V5N9 (SYE_HALMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:rrnAC0085
OrganismHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]
Taxonomic identifier272569 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula

Protein attributes

Sequence length579 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 579579Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119714

Regions

Motif114 – 12411"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
Q5V5N9 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 2B4FF0D9A04C1CB6

FASTA57965,006
        10         20         30         40         50         60 
MDDELRDRIT EAAETNALLN AVKHDSEAQV GAIMGPLMGE NPEFREYGDE IPGVIAPVVE 

        70         80         90        100        110        120 
RVNGMDAEER RERLAELAPE KLEELEAEDE GEDHPLPDLP SAEEYDTVRM RVAPNPNGPW 

       130        140        150        160        170        180 
HIGHARMAAV VGTYKQRYDG EFICRFDDTD PETKRPDLDA YDAILDAIDY LGFEPDDVVN 

       190        200        210        220        230        240 
ASDRVETYYE YARKLIDKGG AYTCSCPQGE FSDLKNNGEA CPHRDKDAET TRSEFEAMVD 

       250        260        270        280        290        300 
GEYDSGEMVL RVRTDITHKN PALRDFVAFR MVDTPHPREA AEQYRCWPML DFQSGLDDHL 

       310        320        330        340        350        360 
LGVTHIIRGI DLQDSAKRQQ FVYDYFDWEY PEVIHWGHVQ VDEYDVPLST SSIAELIEDG 

       370        380        390        400        410        420 
ELAGWDDPRA PTVASLERRG IRGEAVVDAM IQLGTSTSNV DLAMSSIYSN NRDLIDDDSD 

       430        440        450        460        470        480 
RAFFVRDSDD HGGLVERQIV GGPDAGEPPL HPDYEERGRR EIPVTSGVVV EGDDLPDHGD 

       490        500        510        520        530        540 
RIWLKGYGCV RHTRDAFEYT GDDIDAVREE GVDVIHWAPA DGPALRLRTM DGDVSGVAEP 

       550        560        570 
GLRNYDTDDV VQFERIGFAR LDKVADDSDT ESVAYFTHP 

« Hide

References

[1]"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea."
Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V.
Genome Res. 14:2221-2234(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY596297 Genomic DNA. Translation: AAV45163.1.
RefSeqYP_134869.1. NC_006396.1.

3D structure databases

ProteinModelPortalQ5V5N9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272569.rrnAC0085.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV45163; AAV45163; rrnAC0085.
GeneID3128917.
KEGGhma:rrnAC0085.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAAFRIIDT.

Enzyme and pathway databases

BioCycHMAR272569:GJDH-79-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR007527. Znf_SWIM.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_HALMA
AccessionPrimary (citable) accession number: Q5V5N9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: December 7, 2004
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries