Q5V545 (ILVD_HALMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	rrnAC0302

Organism

Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

272569 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103538
Sites
	Metal binding	134	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	206	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5V545 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 204A276BDF77C459	FASTA	575	61,106
	10 20 30 40 50 60 MSNQERQERP EKDPDLRSTE VTEGYEKAPH RAMFRAMGYD DEDLSSPMIG VANPAADITP 70 80 90 100 110 120 CNVHLDDVAD AAYDGIDDTE GMPIEFGTIT ISDAISMGTE GMKASLISRE IIADSVELVT 130 140 150 160 170 180 FGERMDGIVT IGGCDKNMPG MMMAAIRTDL PSVFLYGGSI MPGEHDGREV TIQNVFEGVG 190 200 210 220 230 240 AVADGEMSEG ELDEMERHAC PGAGSCGGMF TANTMASISE ALGFAPLGSA SPPAEHESRY 250 260 270 280 290 300 EEARRAGELA VEVVQERRSP SDFLTRESFE NAIALQVAVG GSTNAVLHLL ALAAEAGIDL 310 320 330 340 350 360 DIETFNEISA RTPKIADLQP GGERVMNDLH EVGGVPVVLR ALNDAGLLHG DALTVTGNTI 370 380 390 400 410 420 AEELEQIDPP TVEDLDVDYL NTVEDPIHER GAIRILSGNL APDGAVIKIT GEDHLHHEGP 430 440 450 460 470 480 VRVFEQEEGA MEYVQEGHVE SGDVICIRNE GPQGGPGMRE MLGVTSAVAG QGHAEDVALF 490 500 510 520 530 540 TDGRFSGATR GFSIGHVAPE AFVGGPIAAL EDGDTITIDI DDHELSVDLT EDEMQQRLEG 550 560 570 HDPEPTYDSG VLAKYHNDFG SAANGAVTNP GAKWD

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY596297 Genomic DNA. Translation: AAV45357.1.
RefSeq	YP_135063.1. NC_006396.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3129693.
GenomeReviews	Gene locus rrnAC0302 in contig AY596297_GR.
KEGG	hma:rrnAC0302.
NMPDR	fig|272569.1.peg.320.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	Q5V545.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC0302-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_HALMA

Accession

Primary (citable) accession number: Q5V545

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	December 7, 2004
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program