Q5V520 (ILVC_HALMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	rrnAC0332

Organism

Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

272569 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000226216
Sites
	Active site	112	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5V520 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 02341F17E5B7DFBA	FASTA	343	37,488
	10 20 30 40 50 60 MSDELTTTVY YDEDADVSTI NDETVAVLGY GSQGHAHALN LHESGVDVIV GLRQDSSSWA 70 80 90 100 110 120 DAEDAGLRVE TPDVAAGEAD RVVMLVPDTI QPAVYEAIED ELDAGDTLQF AHGFNIHYGQ 130 140 150 160 170 180 IEPPEDVDVT MVAPKSPGHL VRRTYERGEG TPGLIAVYQD ATGNAKQESL AYAKGIGCTR 190 200 210 220 230 240 AGVIETSFQE EVETDLFGEQ AVLCGGVTEM VKAGFETLVD AGYAPEMAYF ECLNELKLIV 250 260 270 280 290 300 DLMYEGGHMG MWNSVSDTAE YGGLTRGEEV IDREGMEKIL EEVQNGEFAR EWINENQANR 310 320 330 340 PAYKQYRDAE QNHQIEAVGE NLRELFAWGE DADAETTEAP ADD

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY596297 Genomic DNA. Translation: AAV45382.1.
RefSeq	YP_135088.1. NC_006396.1.
3D structure databases
ProteinModelPortal	Q5V520.
SMR	Q5V520. Positions 8-328.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3129072.
GenomeReviews	Gene locus rrnAC0332 in contig AY596297_GR.
KEGG	hma:rrnAC0332.
NMPDR	fig|272569.1.peg.345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286525.
OMA	MAYAKGI.
PhylomeDB	Q5V520.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC0332-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_HALMA

Accession

Primary (citable) accession number: Q5V520

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	December 7, 2004
Last modified:	December 14, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program