Q5V4R5 (TOP6B_HALMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type 2 DNA topoisomerase 6 subunit B
EC=5.99.1.3

Alternative name(s):
Type II DNA topoisomerase VI subunit B
Short name=TopoVI-B

Gene names

Name:	top6B
Ordered Locus Names:	rrnAC0457

Organism

Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

272569 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula › Haloarcula marismortui

Protein attributes

Sequence length	796 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Relaxes both positive and negative superturns and exhibits a strong decatenase activity By similarity. HAMAP-Rule MF_00322
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_00322
Subunit structure	Homodimer. Heterotetramer of two Top6A and two Top6B chains By similarity.
Sequence similarities	Belongs to the TOP6B family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 796	796	Type 2 DNA topoisomerase 6 subunit B HAMAP-Rule MF_00322		PRO_1000005869
Regions
	Nucleotide binding	109 – 110	2	ATP By similarity
	Nucleotide binding	119 – 126	8	ATP By similarity
Sites
	Binding site	57	1	ATP By similarity
	Binding site	88	1	ATP By similarity
	Binding site	627	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5V4R5 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: DC4FC12432E15C99	FASTA	796	87,759
	10 20 30 40 50 60 MTSYQSQLGE GEGIAEELAE SQRAISIAEF FEKNKHMLGF DSEARALVTA VKEAVDNALD 70 80 90 100 110 120 ACEEAGILPD IYVEIQESGD YYKLVVEDNG PGITKEQAPK IFGKLLYGSR FHAREQNRGQ 130 140 150 160 170 180 QGIGISAAVL YSQLTSGKPA KITSRPKGQD EAQYFELIVD TDTNEPEISV DETTTWERPH 190 200 210 220 230 240 GTRIELEMEA NMRARSTLRD YIQDTAVVNP HARVEFDEPG LDESLKFERA ERAELPDETE 250 260 270 280 290 300 EIRPHPHGVE LGTLLKMLEA TDSYSVSGFM QEEFTRVGGK TADSVIANFN DRHYGRGMAW 310 320 330 340 350 360 QPPKVNEDAD IERAVEDAVA NKGAETTATF AAAVSDAIHD RDRVAYHEVE SIVDSAAEDA 370 380 390 400 410 420 EADGDTTFGA TVRENAVDAA WNAVSDNLSS DLYALVDDVT TKRKDDAAVE GLASRLADKF 430 440 450 460 470 480 DDDGRHRLTR DELRGYIDRA ADMTEEQDDA TFGETARENV LEALWTAAEG VREEPPKVSD 490 500 510 520 530 540 IADDRDTASQ LLSAMRETDI IAPPTDCLSP ISAELVEEGL RKEFDADFYA ASTRDASVHG 550 560 570 580 590 600 GDPFIVEAGI AYGGELESEG TVDVMRFANR VPLVYQRGAC ATTDVVKSIR WRNYNLDQPG 610 620 630 640 650 660 GSGIPKGPAV IMVHVASTNV PFTSESKDAI ANVPEMEDEI ELAIREAARE LKSYLNKRRS 670 680 690 700 710 720 MQQRREKQDK LATILPEMAE KLTEVTDNDE LHIDDSLARI MNNVLVEREV EDDTVRVRIE 730 740 750 760 770 780 NNDDTNADVE LTDIVTAEPQ VTNGATVVEM DGEWFVKWSP TVGAGETAVL EYSVTDEAEF 790 TVSVDGIEEE KLTVNA

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY596297 Genomic DNA. Translation: AAV45487.1.
RefSeq	YP_135193.1. NC_006396.1.
3D structure databases
HSSP	HSSP built from PDB template 1MU5 based on UniProtKB O05207.
ProteinModelPortal	Q5V4R5.
ModBase	Search...
Protein-protein interaction databases
STRING	272569.rrnAC0457.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV45487; AAV45487; rrnAC0457.
GeneID	3130625.
KEGG	hma:rrnAC0457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1389.
HOGENOM	HOG000225919.
KO	K03167.
OMA	LNKRRSM.
ProtClustDB	PRK14868.
Enzyme and pathway databases
BioCyc	HMAR272569:GJDH-406-MONOMER.
Family and domain databases
Gene3D	3.30.230.10. 1 hit. 3.30.565.10. 1 hit.
HAMAP	MF_00322. Top6B.
InterPro	IPR003594. HATPase_ATP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005734. TopoVI_B. IPR015320. TopoVI_B_transducer. [Graphical view]
Pfam	PF02518. HATPase_c. 1 hit. PF09239. Topo-VIb_trans. 1 hit. [Graphical view]
ProDom	PD013790. TopoVI_B_transducer. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01052. top6b. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TOP6B_HALMA

Accession

Primary (citable) accession number: Q5V4R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 7, 2004
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program