Q5V4I4 (GLPK_HALMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	rrnAC0550

Organism

Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

272569 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Glycerol kinase HAMAP MF_00186		PRO_0000059526
Regions
	Nucleotide binding	421 – 425	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity
	Binding site	277	1	ATP By similarity
	Binding site	321	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5V4I4 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 075A117389FDAEBD	FASTA	510	56,406
	10 20 30 40 50 60 MADTYVGAID QGTTGTRFMV FDHSGQVVAN AYEKHEQIYP EPGWVEHDPV EIWENTQEVV 70 80 90 100 110 120 TKGLADAGVG AEQLEALGIT NQRETTIVWD KETGKPVHNA LVWQDRRTTD RVEEIQEEDK 130 140 150 160 170 180 VEWIRGKTGL ECDAYFSATK TEWILDNAEP LKMQSSRGAD LRERAEDGEL LMGTIDAWLI 190 200 210 220 230 240 YKLTGNHITD VSNASRTMLY NIHDMEWDDE LLEEFGVPES MVPEVRPSSD ESLYGHTDAD 250 260 270 280 290 300 GFLKEEVPVA GALGDQQAAL FGQTCFDKGD AKNTYGTGAF YLMNTGSEAV ASDNGLLTTV 310 320 330 340 350 360 GFQMSGEPVQ YALEGSIFIA GAAIEWLEDV DLINNAAQTA ELARSVESTD GVYMVPAFTG 370 380 390 400 410 420 LGAPHWDGRA RGTIVGMTRG TRKEHIVRAT LESIAYQTRD LAEAMEEDSG VEMTTLRVDG 430 440 450 460 470 480 GAVKNNFLCQ LQSDIIQTDI ARPQVDETTA LGSAYAAGLA VGYWDTVDEL RDNWQVDEEF 490 500 510 SPEMDAGKAD KMYARWDDAV DRSRDWAQEE

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY596297 Genomic DNA. Translation: AAV45568.1.
RefSeq	YP_135274.1. NC_006396.1.
3D structure databases
ProteinModelPortal	Q5V4I4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3130507.
GenomeReviews	Gene locus rrnAC0550 in contig AY596297_GR.
KEGG	hma:rrnAC0550.
NMPDR	fig|272569.1.peg.533.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	ANAYETH.
PhylomeDB	Q5V4I4.
ProtClustDB	CLSK511574.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC0550-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_HALMA

Accession

Primary (citable) accession number: Q5V4I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	December 7, 2004
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program