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Q5V3T2 (MEND_HALMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:rrnAC0838
OrganismHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]
Taxonomic identifier272569 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula

Protein attributes

Sequence length592 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence caution

The sequence AAV45820.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5925922-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_0000341896

Sequences

Sequence LengthMass (Da)Tools
Q5V3T2 [UniParc].

Last modified July 1, 2008. Version 2.
Checksum: 1CD9A0AC89160526

FASTA59263,302
        10         20         30         40         50         60 
MTAPNVNTLW AETLVGELVA GGVDAVCLSP GSRSTPLTVA FAEHPDIEVF SHLDERSAAF 

        70         80         90        100        110        120 
FALGRARRTG EPTPLVCTSG TAAANYHPAV IEANQSGVPL LLLTADRPPE LIDSGANQTV 

       130        140        150        160        170        180 
DQEKLYGDAV RWYRDMPEPE AEPRKVRMLR TTAARALAES TGSDPGPVHL NCRFRKPLEP 

       190        200        210        220        230        240 
TPMPEDDPAG VPADWAGGDN GAKIGRDGPF VTTSEGVETP DEQTVRRVQD ALEAAERGLI 

       250        260        270        280        290        300 
VAGPADQGLS ADSLERLAAA TGFPVLADPL SDLRFGPHVD RLDVPVCGGY DGYLGSDSVD 

       310        320        330        340        350        360 
QWDDPDVVVR FGASPTSKPL RHYLRDADCQ QFLVDPAGGW SEAEFTATNL LVANPDATAD 

       370        380        390        400        410        420 
ALAGETLGGV AASWREQFIR AEQTHWDAVT ETASETYWEG GVLSDVTALA PDPATLFISN 

       430        440        450        460        470        480 
SMPIRDMDRF GGPRDADLTV LGNRGASGID GITSTALGAG SATSDPLVLV TGDLAYYHDM 

       490        500        510        520        530        540 
NGLLALGRCA VDATVVLLNN DGGGIFHMLP IEDHPTFEDQ FRTPHGLDFE PTEALYSLQF 

       550        560        570        580        590 
ERVADRRQFR ERFAESVQTD GTQVIEVRFD AGDSHAVRDQ LTEQVAETLA GD 

« Hide

References

[1]"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea."
Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V.
Genome Res. 14:2221-2234(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY596297 Genomic DNA. Translation: AAV45820.1. Different initiation.
RefSeqYP_135526.1. NC_006396.1.

3D structure databases

ProteinModelPortalQ5V3T2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272569.rrnAC0838.

Protocols and materials databases

DNASU3129325.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV45820; AAV45820; rrnAC0838.
GeneID3129325.
KEGGhma:rrnAC0838.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
HOGENOMHOG000218359.
KOK02551.
ProtClustDBCLSK878560.

Enzyme and pathway databases

BioCycHMAR272569:GJDH-745-MONOMER.
UniPathwayUPA00079; UER00164.

Family and domain databases

HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_HALMA
AccessionPrimary (citable) accession number: Q5V3T2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: February 19, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways