Reviewed, UniProtKB/Swiss-Prot Q5V3R2 (DYR1_HALMA)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase 1
    EC=1.5.1.3

Gene names

Name:	folA1
Ordered Locus Names:	rrnAC0859

Organism

Haloarcula marismortui (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

2238 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Dihydrofolate reductase 1		PRO_0000186431
Regions
	Domain	8 – 185	178	DHFR

Sequences

	Sequence		Length	Mass (Da)
	Q5V3R2-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 943E45089F069380	FASTA	185	20,284
	10 20 30 40 50 60 MTMIPDTELV LVVAADENNV IGLDGGVPWH YPEDVRQYKN RIAGHPIILG RRTFESMKPI 70 80 90 100 110 120 PDCYTVVLTS DDRRSADSET VEYATTPQIA VEAAARAGAS GAFAGDSAGA DSSPPVTYVI 130 140 150 160 170 180 GGEAVYDLFL PFAGRVFLSR IHEHNEGDRY FPDLGAEWTE LSREPHDGFD VIEYEQASPR PLDDL

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY596297 Genomic DNA. Translation: AAV45840.1.
RefSeq	YP_135546.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3130204.
GenomeReviews	Gene locus rrnAC0859 in contig AY596297_GR.
KEGG	hma:rrnAC0859.
NMPDR	fig|272569.1.peg.810.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5V3R2.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC0859-MON.
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR1_HALMA

Accession

Primary (citable) accession number: Q5V3R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	December 7, 2004
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)