Q5V2D3 (PURL_HALMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	rrnAC1390

Organism

Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

272569 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100510
Regions
	Nucleotide binding	96 – 107	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5V2D3 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: FAC42D34B0831147	FASTA	720	75,951
	10 20 30 40 50 60 MSLSDADHEL VVEEIGREPT RAEAALFENL WSEHCAYRSS RPLLSAFDSE GDQVVIGPGD 70 80 90 100 110 120 DAAVVSLPSH GDGEEMYITM GVESHNHPSY VDPFDGAATG VGGIVRDTLS MGAYPIALAD 130 140 150 160 170 180 CLYFGDFDRE HSRYLFEGVV EGISHYGNCI GVPTVTGSVA FHDDYEGNPL VNVSCIGLLE 190 200 210 220 230 240 PERTITAEAQ EPGNKLVLVG NATGRDGLGG ASFASEDLAE DAETEDRPAV QVGDPYSEKL 250 260 270 280 290 300 LVECNEALLD EELVESARDL GAAGLGGASS ELIAKGGLGA RIELDRVHER EPNMNAMEYL 310 320 330 340 350 360 LAESQERMVY EVAPEDVDRV AELAERFDLG CSVIGELTEP GTNYVCTFEG ETVVDVDAAF 370 380 390 400 410 420 LGDGAPMNDL PSDAPPKQER DLPTVSLDEA FERIVSSPNC ASKRWVYRQY DHEVQVRTSV 430 440 450 460 470 480 LPGDDAALLA IREAGTGLAF SAGADPNWTD AAPYEGARAV ALENATNVAA KGATPHAAVD 490 500 510 520 530 540 CLNGGNPEKP DVYGGFKGIV DGLADMCSDL DVPVVGGNVS LYNDSQDGPI PPTPTLALVG 550 560 570 580 590 600 VKEGYDAPPL SLSGEGRLVV VGDTALEGKT DPRLGGSEYT AQFGGTDRFP ALPVDSTDVV 610 620 630 640 650 660 ETIAEVADAD HVLASHDVSH GGLAVTLAEM VHEDAGASVE IGTTEHGTPA RLLFNERPGR 670 680 690 700 710 720 VVFETTDPAA VREAFDGVAP VTELGEANDS NGLDITVNDE TLAYNVADIA DLRSVIDDEL

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY596297 Genomic DNA. Translation: AAV46319.1.
RefSeq	YP_136025.1. NC_006396.1.
3D structure databases
ProteinModelPortal	Q5V2D3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3130326.
GenomeReviews	Gene locus rrnAC1390 in contig AY596297_GR.
KEGG	hma:rrnAC1390.
NMPDR	fig|272569.1.peg.1290.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q5V2D3.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC1390-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_HALMA

Accession

Primary (citable) accession number: Q5V2D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	December 7, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program