Reviewed, UniProtKB/Swiss-Prot Q5V1C1 (HUTI_HALMA)

Names and origin

Protein names

Recommended name:
    Probable imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	rrnAC1790

Organism

Haloarcula marismortui (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

2238 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Probable imidazolonepropionase HAMAP MF_00372		PRO_0000306545
Sites
	Metal binding	77	1	Zinc or iron By similarity
	Metal binding	79	1	Zinc or iron By similarity
	Metal binding	245	1	Zinc or iron By similarity
	Metal binding	319	1	Zinc or iron By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	149	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5V1C1-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: BAB430084882FB3A	FASTA	420	43,490
	10 20 30 40 50 60 MTTLDAVVYG ADELVAGPAT DGRRLETYED GAVAIVDGTV AAVGPTTEVT AEYPPESANH 70 80 90 100 110 120 AVDADGQAVI PGFVDPHTHA LFAGDRSDEF AAKLRGKSYQ DILAEGGGIL RTVRAVRDAD 130 140 150 160 170 180 EETLLSNLLA HLDTMLAHGT TTVEVKSGYG LDTETELQML RVIDSADAVH PVDVVPTFMG 190 200 210 220 230 240 AHAVPEGWDT DDYTAAVVDE QVPAVESQGI AEFCDVFCEE GVFSVAQSRR VLEAGAAAGL 250 260 270 280 290 300 TPKVHAEELA HIGGTQLAAD VGAASADHLL HATGEDIDAL VDAAVTPVLL PGTAFGLGAA 310 320 330 340 350 360 YADATAFRDR GAPVAVATDF NPNCHSHSMG FALSLACVEM GLTPAEALIA GTKHAALALD 370 380 390 400 410 420 RTDGLGTLRE GAPGDAVVLA APSHVHIPYQ YGTNVVDAIL KDGSVVSTPT HESYDREVQP

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY596297 Genomic DNA. Translation: AAV46682.1.
RefSeq	YP_136388.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3128946.
GenomeReviews	Gene locus rrnAC1790 in contig AY596297_GR.
KEGG	hma:rrnAC1790.
NMPDR	fig|272569.1.peg.1658.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5V1C1.
OMA	MNMACTL.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC1790-MON.
BRENDA	3.5.2.7. 88.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_HALMA

Accession

Primary (citable) accession number: Q5V1C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	December 7, 2004
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)