Reviewed, UniProtKB/Swiss-Prot Q5V0S5 (KATG1_HALMA)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	rrnAC2018

Organism

Haloarcula marismortui (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

2238 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354965
Sites
	Active site	93	1	Proton acceptor By similarity
	Metal binding	258	1	Iron (heme axial ligand) By similarity
	Site	89	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	92 ↔ 217		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-243) By similarity
	Cross-link	217 ↔ 243		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-92) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5V0S5-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 490649C67F89CCA4	FASTA	730	81,147
	10 20 30 40 50 60 MLKTVLMPSP SKCSLMAKRD QDWSPNQLRL DILDQNARDA DPRGTGFDYA EEFQELDLDA 70 80 90 100 110 120 VKADLEELMT SSQDWWPADY GHYGPLFIRM AWHSAGTYRT TDGRGGASGG RQRFAPLNSW 130 140 150 160 170 180 PDNANLDKAR RLLWPIKKKY GRKLSWADLI VLAGNHAIES MGLKTFGWAG GREDAFEPDE 190 200 210 220 230 240 AVDWGPEDEM EAHQSERRTD DGELKEPLGA AVMGLIYVDP EGPNGNPDPL ASAENIRESF 250 260 270 280 290 300 GRMAMNDEET AALIAGGHTF GKVHGADDPE ENLGDVPEDA PIEQMGLGWE NDYGSGKAGD 310 320 330 340 350 360 TITSGIEGPW TQAPIEWDNG YIDNLLDYEW EPEKGPGGAW QWTPTDEALA NTVPDAHDPS 370 380 390 400 410 420 EKQTPMMLTT DIALKRDPDY REVMERFQEN PMEFGINFAR AWYKLIHRDM GPPERFLGPD 430 440 450 460 470 480 APDEEMIWQD PVPDVDHDLI GDEEVAELKT DILETDLTVS QLVKTAWASA STYRDSDKRG 490 500 510 520 530 540 GANGARIRLE PQKNWEVNEP AQLETVLATL EEIQAEFNSA RTDDTRVSLA DLIVLGGNAA 550 560 570 580 590 600 VEQAAADAGY DVTVPFEPGR TDATPEQTDV DSFEALKPRA DGFRNYARDD VDVPAEELLV 610 620 630 640 650 660 DRADLLDLTP EEMTVLVGGL RSLGATYQDS DLGVFTDEPG TLTNDFFEVV LGMDTEWEPV 670 680 690 700 710 720 SESKDVFEGY DRETGEQTWA ASRVDLIFGS HSRLRAIAEV YGADGAEAEL VDDFVDAWHK 730 VMRLDRFDLE

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY596297 Genomic DNA. Translation: AAV46878.1.
RefSeq	YP_136584.1.
3D structure databases
ModBase	Search...
Protein family/group databases
PeroxiBase	3064. HmaCP02.
Genome annotation databases
GeneID	3129956.
GenomeReviews	Gene locus rrnAC2018 in contig AY596297_GR.
KEGG	hma:rrnAC2018.
NMPDR	fig|272569.1.peg.1861.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5V0S5.
OMA	Q5V0S5. FEWELTK.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC2018-MON.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_HALMA

Accession

Primary (citable) accession number: Q5V0S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)