Reviewed, UniProtKB/Swiss-Prot Q5V061 (G3P_HALMA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	rrnAC2262

Organism

Haloarcula marismortui (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

2238 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000232388
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	137 – 139	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	192 – 193	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	138	1	Nucleophile By similarity
	Binding site	108	1	NAD; via amide nitrogen By similarity
	Binding site	166	1	NAD By similarity
	Binding site	299	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5V061-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 8FE722C4EC560A59	FASTA	354	37,924
	10 20 30 40 50 60 MLHVGINGFG TIGKRVADAV RVQPDMTVAG VAKRSPNFEA TIADDRGYDL YAADGREPFD 70 80 90 100 110 120 EADLGTAGTV HDLIETSDVV VDTTPSGVGA ANASLYAEHD TPAIFQGGED ADVADVSFNA 130 140 150 160 170 180 RANYEKAVGA DTARVVSCNT TGLSRLLAPL RESYGVEKSR VTLVRRGADP GQTGRGPIND 190 200 210 220 230 240 TLPDPVEIPS HHGPDVQTIF PDLDIDTMGM KVPTTQMHTH SVNVTLESEP ATEEVTALLA 250 260 270 280 290 300 DESRLFLIPE TLGIDGAGKL KEYTRDAGRP RGDVWENCIW AESITVEGRD LYLFQAIHQE 310 320 330 340 350 ADVVPENIDA VRALSERTAS AEKSIRRTDE ALGVGRGLVE HDGSPQRVDS HADD

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY596297 Genomic DNA. Translation: AAV47092.1.
RefSeq	YP_136798.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3128511.
GenomeReviews	Gene locus rrnAC2262 in contig AY596297_GR.
KEGG	hma:rrnAC2262.
NMPDR	fig|272569.1.peg.2078.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5V061.
OMA	Q5V061. AIFQGGE.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC2262-MON.
BRENDA	1.2.1.59. 88.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006436. Glyceraldehyde-3-P_DH_2_arc. [Graphical view]
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_HALMA

Accession

Primary (citable) accession number: Q5V061

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)