Q5UZE6 (SYA_HALMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	rrnAC2561

Organism

Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

272569 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	927 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 927	927	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000075261
Sites
	Metal binding	618	1	Zinc By similarity
	Metal binding	622	1	Zinc By similarity
	Metal binding	721	1	Zinc By similarity
	Metal binding	725	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UZE6 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 5E45BC5834309669	FASTA	927	102,893
	10 20 30 40 50 60 MSDLDEAYQL DYFEEEGFHR QECVSCGDMF WSRVKRETCG EPPCAEYDFI DNPGFDESHS 70 80 90 100 110 120 LTEMREAFLS FFEDRDHERI DPYPVAANRW RDDVLLTQAS IYDFQPLVTS GTTPPPANPL 130 140 150 160 170 180 CISQPCIRMQ DIDNVGKTGR HTMAFEMMAH HAFNAREDIE DPEQYAYEGE VYWKNETVQY 190 200 210 220 230 240 CDELFEEMGA NLDEIVYIED PWVGGGNAGP AIEVIYRGAE LATLVFMSME QDSDGEYEMK 250 260 270 280 290 300 DGNRYSPMDT YIVDTGYGLE RWTWMSQGTP TVYEAVYPDM IAFLKDNAGI ELTDEEERIV 310 320 330 340 350 360 HEAAKLAGRM DIDEAEDIEA ERDTIAAEVG VEVEEMRDLM APLEDIYAIA DHCRTLAYML 370 380 390 400 410 420 GDGIVPSNVG TGYLARMVLR RTKRLVDGVG VDAPLDELVD MQAERLDYEN RDTIRDIVRT 430 440 450 460 470 480 EVEKYRETLD RGGRRVRQLA DEYAEKGEPI PTSELVELYD SHGIQPDMVE EIAAEKGVAV 490 500 510 520 530 540 DVPDDFYAVV AQRHGGDDTA TEESQTPYED RLEELPETDR LYYEDQQRTE FEAVVLEVFD 550 560 570 580 590 600 RDEDTYDVVL DQTMFYPEGG GQPPDKGTLS TDDVSAEVTD VQQYGDVIVH TCDDDPGKGE 610 620 630 640 650 660 FVRGQVDATR RQRLMQHHTA THIVIHSARQ VLGEHVRQAG AQKGTDSARI DIRHYESVSR 670 680 690 700 710 720 EKVKEIERLA NDIIQDNITV SQEWPDRNDA EERYGFDLYQ GGIPAGEQIR LITVGDDVQA 730 740 750 760 770 780 CGGTHVARTG DIGAVKLLNT ERIQDGVIRL TFAAGNAAIE ATQRTEDALT EAADILDVAP 790 800 810 820 830 840 DAVPETAERF FDEWKARGKE IEQLKEQLAE ARASGGGDNE EVGVGDATAV VGRIDADMDE 850 860 870 880 890 900 LRAQANAIVE QGNIAVLGSG LDGAQFVVSV PDGVDVDAGE VVGELAGRVG GGGGGPPDFA 910 920 QGGGPDADAL DDALDDAPEI LRTVANA

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY596297 Genomic DNA. Translation: AAV47357.1.
RefSeq	YP_137063.1. NC_006396.1.
3D structure databases
ProteinModelPortal	Q5UZE6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3128743.
GenomeReviews	Gene locus rrnAC2561 in contig AY596297_GR.
KEGG	hma:rrnAC2561.
NMPDR	fig|272569.1.peg.2344.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	Q5UZE6.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC2561-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_HALMA

Accession

Primary (citable) accession number: Q5UZE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	December 7, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program