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Q5UZ84

- SYI_HALMA

UniProt

Q5UZ84 - SYI_HALMA

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Protein

Isoleucine--tRNA ligase

Gene
ileS, rrnAC2634
Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei608 – 6081ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-2375-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:rrnAC2634
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
ProteomesiUP000001169: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10741074Isoleucine--tRNA ligaseUniRule annotationPRO_0000098576Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272569.rrnAC2634.

Structurei

3D structure databases

ProteinModelPortaliQ5UZ84.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionUniRule annotationAdd
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRYVEGKW.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5UZ84-1 [UniParc]FASTAAdd to Basket

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MERFAAVDDQ YDPDAVEDGV FEYWDDVDAY EQTKAHRADG EDYFFVDGPP     50
YTSGAAHMGT TWNKTLKDCY IRYLRMQGYN VTDRPGYDMH GLPIETKVEE 100
RLDFENKKDI EQFGEENFIE ECKDFAEEQL EGLQTDFQDF GVWMDWDDPY 150
KTVNPEYMEA AWWGFQQAHE RDLVEQGQRS INQCPRCETG IANNEVEYHD 200
VGKPSIYVKF PLAEQDGSLV IWTTTPWTIV ANTFVAADGD LEYVGVDAEK 250
DGDTERLYLA EACVEDVLKA GRYDDYEVVE ELSGEEMVGW AYEHPLAEEV 300
PDHAQGEGSG QVYTADYVEA DRTGLVHSAP GHGEEDFERG QELDLEIFCP 350
VGSDGVYTDD AGKYAGTFVR DANDEVIDDL DENGVLLSSE PGHTVREGQC 400
WRCDTDIVRI VTDQWFITVT DIKDELLANI EDSEWYPQWA RDNRFRDFVE 450
DAPDWNVSRQ RYWGIPIPIW LPEDWSGDMD DAIVVGDREE LAERVDQDID 500
PENVDLHKGT VDDLTITEDS TTYTRVGDVF DVWLDSSVAT WGTVNYPEQT 550
EDFEELWPAD LIMEAHDQTR GWFWSQLGMS TAATGEIPYK QVLMHGYANM 600
PDGRGMSKSK GVLIDPHEVI EKHGRDPMRL FLLSVTAQGE DMNFSWEETA 650
EMQRRLNILW NVARFPLPYM RADDFDPEET TVEDLRDDLE LVDEWVLSRL 700
QSVTEAMTDS MDDFENDKAV DELLEFVVED VSRFYIQVVR ERMWEEEDSA 750
SKQAAYATLY RVLESVAALF APFTPFVAEQ VYGALTGDAG HPTVHMCDWP 800
EVDADLHDPA LEREIEVVRE VEEAGSNARQ QAERKLRWPV TRVVVDVDSD 850
DVADAVRAQE AIIADRLNAR AVEVVGADDE WGELQYSAEA DMSELGPAFG 900
DDAGRVMNAL NEARVTEQSL DTLEGTVREA LGEDVDLTEE MVEFRRETPE 950
GVTGTEFTAL DGGGVVYVDT ALTEDIESEG YAREVIRRIQ EMRKDLELDI 1000
EERIAVDLTI DDERVDSLVR EHEALIKEEV RADELDGVED GHRKTWEVEG 1050
TDMEIAIAPC EADQREASEQ ASGD 1074
Length:1,074
Mass (Da):122,156
Last modified:December 7, 2004 - v1
Checksum:iD476C7E610567942
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY596297 Genomic DNA. Translation: AAV47419.1.
RefSeqiWP_011224343.1. NC_006396.1.
YP_137125.1. NC_006396.1.

Genome annotation databases

EnsemblBacteriaiAAV47419; AAV47419; rrnAC2634.
GeneIDi3129589.
KEGGihma:rrnAC2634.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY596297 Genomic DNA. Translation: AAV47419.1 .
RefSeqi WP_011224343.1. NC_006396.1.
YP_137125.1. NC_006396.1.

3D structure databases

ProteinModelPortali Q5UZ84.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272569.rrnAC2634.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV47419 ; AAV47419 ; rrnAC2634 .
GeneIDi 3129589.
KEGGi hma:rrnAC2634.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RYVEGKW.

Enzyme and pathway databases

BioCyci HMAR272569:GJDH-2375-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Entry informationi

Entry nameiSYI_HALMA
AccessioniPrimary (citable) accession number: Q5UZ84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 7, 2004
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi