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Q5UZ84

- SYI_HALMA

UniProt

Q5UZ84 - SYI_HALMA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei608 – 6081ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHMAR272569:GJDH-2375-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:rrnAC2634
    OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
    Taxonomic identifieri272569 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
    ProteomesiUP000001169: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10741074Isoleucine--tRNA ligasePRO_0000098576Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272569.rrnAC2634.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5UZ84.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 6011"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRYVEGKW.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5UZ84-1 [UniParc]FASTAAdd to Basket

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    MERFAAVDDQ YDPDAVEDGV FEYWDDVDAY EQTKAHRADG EDYFFVDGPP     50
    YTSGAAHMGT TWNKTLKDCY IRYLRMQGYN VTDRPGYDMH GLPIETKVEE 100
    RLDFENKKDI EQFGEENFIE ECKDFAEEQL EGLQTDFQDF GVWMDWDDPY 150
    KTVNPEYMEA AWWGFQQAHE RDLVEQGQRS INQCPRCETG IANNEVEYHD 200
    VGKPSIYVKF PLAEQDGSLV IWTTTPWTIV ANTFVAADGD LEYVGVDAEK 250
    DGDTERLYLA EACVEDVLKA GRYDDYEVVE ELSGEEMVGW AYEHPLAEEV 300
    PDHAQGEGSG QVYTADYVEA DRTGLVHSAP GHGEEDFERG QELDLEIFCP 350
    VGSDGVYTDD AGKYAGTFVR DANDEVIDDL DENGVLLSSE PGHTVREGQC 400
    WRCDTDIVRI VTDQWFITVT DIKDELLANI EDSEWYPQWA RDNRFRDFVE 450
    DAPDWNVSRQ RYWGIPIPIW LPEDWSGDMD DAIVVGDREE LAERVDQDID 500
    PENVDLHKGT VDDLTITEDS TTYTRVGDVF DVWLDSSVAT WGTVNYPEQT 550
    EDFEELWPAD LIMEAHDQTR GWFWSQLGMS TAATGEIPYK QVLMHGYANM 600
    PDGRGMSKSK GVLIDPHEVI EKHGRDPMRL FLLSVTAQGE DMNFSWEETA 650
    EMQRRLNILW NVARFPLPYM RADDFDPEET TVEDLRDDLE LVDEWVLSRL 700
    QSVTEAMTDS MDDFENDKAV DELLEFVVED VSRFYIQVVR ERMWEEEDSA 750
    SKQAAYATLY RVLESVAALF APFTPFVAEQ VYGALTGDAG HPTVHMCDWP 800
    EVDADLHDPA LEREIEVVRE VEEAGSNARQ QAERKLRWPV TRVVVDVDSD 850
    DVADAVRAQE AIIADRLNAR AVEVVGADDE WGELQYSAEA DMSELGPAFG 900
    DDAGRVMNAL NEARVTEQSL DTLEGTVREA LGEDVDLTEE MVEFRRETPE 950
    GVTGTEFTAL DGGGVVYVDT ALTEDIESEG YAREVIRRIQ EMRKDLELDI 1000
    EERIAVDLTI DDERVDSLVR EHEALIKEEV RADELDGVED GHRKTWEVEG 1050
    TDMEIAIAPC EADQREASEQ ASGD 1074
    Length:1,074
    Mass (Da):122,156
    Last modified:December 7, 2004 - v1
    Checksum:iD476C7E610567942
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY596297 Genomic DNA. Translation: AAV47419.1.
    RefSeqiWP_011224343.1. NC_006396.1.
    YP_137125.1. NC_006396.1.

    Genome annotation databases

    EnsemblBacteriaiAAV47419; AAV47419; rrnAC2634.
    GeneIDi3129589.
    KEGGihma:rrnAC2634.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY596297 Genomic DNA. Translation: AAV47419.1 .
    RefSeqi WP_011224343.1. NC_006396.1.
    YP_137125.1. NC_006396.1.

    3D structure databases

    ProteinModelPortali Q5UZ84.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272569.rrnAC2634.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV47419 ; AAV47419 ; rrnAC2634 .
    GeneIDi 3129589.
    KEGGi hma:rrnAC2634.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RYVEGKW.

    Enzyme and pathway databases

    BioCyci HMAR272569:GJDH-2375-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

    Entry informationi

    Entry nameiSYI_HALMA
    AccessioniPrimary (citable) accession number: Q5UZ84
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3