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Protein

DNA polymerase II large subunit

Gene

polC

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation
Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.UniRule annotation

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA-directed DNA polymerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA catabolic process, exonucleolytic Source: UniProtKB-HAMAP
  2. DNA-dependent DNA replication Source: UniProtKB-HAMAP
  3. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-2420-MONOMER.

Protein family/group databases

MEROPSiN10.006.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase II large subunitUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IIUniRule annotation
Cleaved into the following chain:
Alternative name(s):
Hma pol II intein
Gene namesi
Name:polCUniRule annotation
Synonyms:polA2
Ordered Locus Names:rrnAC2691
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
ProteomesiUP000001169: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 965965DNA polymerase II large subunit, 1st partSequence AnalysisPRO_0000294677Add
BLAST
Chaini966 – 1145180Hma polC inteinSequence AnalysisPRO_0000294678Add
BLAST
Chaini1146 – 1395250DNA polymerase II large subunit, 2nd partSequence AnalysisPRO_0000294679Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heterodimer of a large subunit and a small subunit.UniRule annotation

Protein-protein interaction databases

STRINGi272569.rrnAC2691.

Structurei

3D structure databases

ProteinModelPortaliQ5UZ40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal DNA polymerase II family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1933.
HOGENOMiHOG000286435.
KOiK02322.
OMAiRNCDGDE.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
HAMAPiMF_00324. DNApol_II_L_arch.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamiPF03833. PolC_DP2. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEiPS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5UZ40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREADEQYFE TLETQLEAAF DVAERAKERG GDPKPEVEIP TARDMADRVE
60 70 80 90 100
NILGIDGVAE RVRELEGQMS REEAALELVE DFVEGTVGDY DSREGKVEGA
110 120 130 140 150
VRTAVALLTE GVVAAPIEGI DRVELLENDD GTEFINVYYA GPIRSAGGTA
160 170 180 190 200
QALSVLVADY ARALLGIDQY KAREEEIGRY AEEIDLYDKD TGLQYSPKEK
210 220 230 240 250
ETKFIAEHMP IMLDGEATGD EEVSGYRDLE RVDSNSPRGG MCLVLAEGIA
260 270 280 290 300
LKAPKIQRYT RNLDEVDWPW LQDLIDGTIG KDEADEGDSA EDANGDDAGE
310 320 330 340 350
GADDDGGDEA DEQAGPPRVE PADKYLRDLI AGRPVFSHPS KSGGFRLRYG
360 370 380 390 400
RSRNHGFATA GVHPATMHLV DDFLATGTQI KTERPGKAAG VVPVDTIEGP
410 420 430 440 450
TVRLANGDVR RIDDAEEALA VRNGVEKILD LGEYLVNYGE FVENNHPLAP
460 470 480 490 500
ASYTVEWWEQ DLAAAGADVQ AMQDSPHIDL ADPSAEEAIE WATEYDAPLH
510 520 530 540 550
PKYTYLWHDV SIEQVCALAD AVEDAQVAQA DGAYADPEMD GTAGDAHSDD
560 570 580 590 600
GALVLPRSDA VQQTLEHLLI GHTQDEETIT VTDWVPLVRT LGFSRSLERD
610 620 630 640 650
WTREDLSEHA RTYGESESLD AIGVAEDAER EDGQNAIKAI NEVAPFQVRE
660 670 680 690 700
RAPTRIGNRM GRPEKSERRD LSPAVHTLSP IGEAGGAQRD VAKATKHADD
710 720 730 740 750
MSDTPGQVEV EVARRRCPDC GTETHQANCA ECSGTTEPVY VCPDCEAEVE
760 770 780 790 800
RDESGRAECG RCETLASPTQ YKVLDLQEAY RDALQNVGER ETAFEQLKAV
810 820 830 840 850
KGLTSEEKVP EPMEKGILRA KHDVSAFKDG TVRYDMTDLP VTAVRASELD
860 870 880 890 900
VSAERLRGLG YTEDIHGDPL THEDQLVELK VQDIVLSDGA AEHMLQTARF
910 920 930 940 950
VDDLLEQYYG LERFYEFDDR EDLVGELVFG MAPHTSAATV GRVVGFTSAA
960 970 980 990 1000
VGYAHPYFHA AKRRNCFHPD TRLWYEDEND DWEYGTIEEL VESRLDDPQE
1010 1020 1030 1040 1050
DDFGTLVQEL DGDLTVSSLG ENGPCRQPVD AVSKHPAPDH LVEVAVGDRT
1060 1070 1080 1090 1100
LRVTPDHTML RAGPDGIEEV PASDLAAGDD LPAYDGGETT TMTARGEAST
1110 1120 1130 1140 1150
AATDGAAPTD TVEAVEYVES DVDHVYCLTV ADTHRVAVEG TYVGQCDGDE
1160 1170 1180 1190 1200
DCVMLLMDGL LNFSKSYLPN QRGGQMDAPL VMSSRIDPSE IDDEAHNMDI
1210 1220 1230 1240 1250
MDAYPREFYE ATREMKDPTE VEDVMKIAEE TLGTDREYTE FRHTHDTANI
1260 1270 1280 1290 1300
AAGPDLSAYK TLGSMEDKMD AQLEISRKLR AVVESDVAER IIEYHFLPDL
1310 1320 1330 1340 1350
IGNLRAFSRQ EVRCLDCGES FRRAPLTGDC RECGGRVNLT VHEGSVNKYI
1360 1370 1380 1390
DTAIRVADEF GARDYTKQRL KILERKIESV FENDHNKQSG IADFM
Length:1,395
Mass (Da):153,633
Last modified:December 7, 2004 - v1
Checksum:i99996EC3A54B3F07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY596297 Genomic DNA. Translation: AAV47463.1.
RefSeqiYP_137169.1. NC_006396.1.

Genome annotation databases

EnsemblBacteriaiAAV47463; AAV47463; rrnAC2691.
GeneIDi3128603.
KEGGihma:rrnAC2691.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY596297 Genomic DNA. Translation: AAV47463.1.
RefSeqiYP_137169.1. NC_006396.1.

3D structure databases

ProteinModelPortaliQ5UZ40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272569.rrnAC2691.

Protein family/group databases

MEROPSiN10.006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV47463; AAV47463; rrnAC2691.
GeneIDi3128603.
KEGGihma:rrnAC2691.

Phylogenomic databases

eggNOGiCOG1933.
HOGENOMiHOG000286435.
KOiK02322.
OMAiRNCDGDE.

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-2420-MONOMER.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
HAMAPiMF_00324. DNApol_II_L_arch.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamiPF03833. PolC_DP2. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEiPS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Entry informationi

Entry nameiDP2L_HALMA
AccessioniPrimary (citable) accession number: Q5UZ40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: December 7, 2004
Last modified: March 4, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.