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Q5UZ40 (DP2L_HALMA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase II large subunit

Short name=Pol II
EC=2.7.7.7

Cleaved into the following chain:

  1. Hma polC intein
    Alternative name(s):
    Hma pol II intein
Gene names
Name:polC
Synonyms:polA2
Ordered Locus Names:rrnAC2691
OrganismHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]
Taxonomic identifier272569 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula

Protein attributes

Sequence length1395 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP-Rule MF_00324

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP-Rule MF_00324

Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP-Rule MF_00324

Subunit structure

Heterodimer of a large subunit and a small subunit By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00324

Sequence similarities

Belongs to the archaeal DNA polymerase II family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 965965DNA polymerase II large subunit, 1st part Potential
PRO_0000294677
Chain966 – 1145180Hma polC intein Potential
PRO_0000294678
Chain1146 – 1395250DNA polymerase II large subunit, 2nd part Potential
PRO_0000294679

Sequences

Sequence LengthMass (Da)Tools
Q5UZ40 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 99996EC3A54B3F07

FASTA1,395153,633
        10         20         30         40         50         60 
MREADEQYFE TLETQLEAAF DVAERAKERG GDPKPEVEIP TARDMADRVE NILGIDGVAE 

        70         80         90        100        110        120 
RVRELEGQMS REEAALELVE DFVEGTVGDY DSREGKVEGA VRTAVALLTE GVVAAPIEGI 

       130        140        150        160        170        180 
DRVELLENDD GTEFINVYYA GPIRSAGGTA QALSVLVADY ARALLGIDQY KAREEEIGRY 

       190        200        210        220        230        240 
AEEIDLYDKD TGLQYSPKEK ETKFIAEHMP IMLDGEATGD EEVSGYRDLE RVDSNSPRGG 

       250        260        270        280        290        300 
MCLVLAEGIA LKAPKIQRYT RNLDEVDWPW LQDLIDGTIG KDEADEGDSA EDANGDDAGE 

       310        320        330        340        350        360 
GADDDGGDEA DEQAGPPRVE PADKYLRDLI AGRPVFSHPS KSGGFRLRYG RSRNHGFATA 

       370        380        390        400        410        420 
GVHPATMHLV DDFLATGTQI KTERPGKAAG VVPVDTIEGP TVRLANGDVR RIDDAEEALA 

       430        440        450        460        470        480 
VRNGVEKILD LGEYLVNYGE FVENNHPLAP ASYTVEWWEQ DLAAAGADVQ AMQDSPHIDL 

       490        500        510        520        530        540 
ADPSAEEAIE WATEYDAPLH PKYTYLWHDV SIEQVCALAD AVEDAQVAQA DGAYADPEMD 

       550        560        570        580        590        600 
GTAGDAHSDD GALVLPRSDA VQQTLEHLLI GHTQDEETIT VTDWVPLVRT LGFSRSLERD 

       610        620        630        640        650        660 
WTREDLSEHA RTYGESESLD AIGVAEDAER EDGQNAIKAI NEVAPFQVRE RAPTRIGNRM 

       670        680        690        700        710        720 
GRPEKSERRD LSPAVHTLSP IGEAGGAQRD VAKATKHADD MSDTPGQVEV EVARRRCPDC 

       730        740        750        760        770        780 
GTETHQANCA ECSGTTEPVY VCPDCEAEVE RDESGRAECG RCETLASPTQ YKVLDLQEAY 

       790        800        810        820        830        840 
RDALQNVGER ETAFEQLKAV KGLTSEEKVP EPMEKGILRA KHDVSAFKDG TVRYDMTDLP 

       850        860        870        880        890        900 
VTAVRASELD VSAERLRGLG YTEDIHGDPL THEDQLVELK VQDIVLSDGA AEHMLQTARF 

       910        920        930        940        950        960 
VDDLLEQYYG LERFYEFDDR EDLVGELVFG MAPHTSAATV GRVVGFTSAA VGYAHPYFHA 

       970        980        990       1000       1010       1020 
AKRRNCFHPD TRLWYEDEND DWEYGTIEEL VESRLDDPQE DDFGTLVQEL DGDLTVSSLG 

      1030       1040       1050       1060       1070       1080 
ENGPCRQPVD AVSKHPAPDH LVEVAVGDRT LRVTPDHTML RAGPDGIEEV PASDLAAGDD 

      1090       1100       1110       1120       1130       1140 
LPAYDGGETT TMTARGEAST AATDGAAPTD TVEAVEYVES DVDHVYCLTV ADTHRVAVEG 

      1150       1160       1170       1180       1190       1200 
TYVGQCDGDE DCVMLLMDGL LNFSKSYLPN QRGGQMDAPL VMSSRIDPSE IDDEAHNMDI 

      1210       1220       1230       1240       1250       1260 
MDAYPREFYE ATREMKDPTE VEDVMKIAEE TLGTDREYTE FRHTHDTANI AAGPDLSAYK 

      1270       1280       1290       1300       1310       1320 
TLGSMEDKMD AQLEISRKLR AVVESDVAER IIEYHFLPDL IGNLRAFSRQ EVRCLDCGES 

      1330       1340       1350       1360       1370       1380 
FRRAPLTGDC RECGGRVNLT VHEGSVNKYI DTAIRVADEF GARDYTKQRL KILERKIESV 

      1390 
FENDHNKQSG IADFM 

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References

[1]"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea."
Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V.
Genome Res. 14:2221-2234(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY596297 Genomic DNA. Translation: AAV47463.1.
RefSeqYP_137169.1. NC_006396.1.

3D structure databases

ProteinModelPortalQ5UZ40.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272569.rrnAC2691.

Protein family/group databases

MEROPSN10.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV47463; AAV47463; rrnAC2691.
GeneID3128603.
KEGGhma:rrnAC2691.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1933.
HOGENOMHOG000286435.
KOK02322.
OMARNCDGDE.

Enzyme and pathway databases

BioCycHMAR272569:GJDH-2420-MONOMER.

Family and domain databases

Gene3D2.170.16.10. 1 hit.
HAMAPMF_00324. DNApol_II_L_arch.
InterProIPR028992. Hedgehog/Intein_dom.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamPF03833. PolC_DP2. 1 hit.
[Graphical view]
SUPFAMSSF51294. SSF51294. 1 hit.
TIGRFAMsTIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEPS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDP2L_HALMA
AccessionPrimary (citable) accession number: Q5UZ40
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: December 7, 2004
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries