Q5UZ40 (DP2L_HALMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Cleaved into the following chain:

1. Hma polC intein
   Alternative name(s):
   Hma pol II intein

Gene names

Name:	polC
Synonyms:	polA2
Ordered Locus Names:	rrnAC2691

Organism

Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

272569 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	1395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 965	965	DNA polymerase II large subunit, 1st part Potential		PRO_0000294677
	Chain	966 – 1145	180	Hma polC intein Potential		PRO_0000294678
	Chain	1146 – 1395	250	DNA polymerase II large subunit, 2nd part Potential		PRO_0000294679

Sequences

	Sequence		Length	Mass (Da)
	Q5UZ40 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 99996EC3A54B3F07	FASTA	1,395	153,633
	10 20 30 40 50 60 MREADEQYFE TLETQLEAAF DVAERAKERG GDPKPEVEIP TARDMADRVE NILGIDGVAE 70 80 90 100 110 120 RVRELEGQMS REEAALELVE DFVEGTVGDY DSREGKVEGA VRTAVALLTE GVVAAPIEGI 130 140 150 160 170 180 DRVELLENDD GTEFINVYYA GPIRSAGGTA QALSVLVADY ARALLGIDQY KAREEEIGRY 190 200 210 220 230 240 AEEIDLYDKD TGLQYSPKEK ETKFIAEHMP IMLDGEATGD EEVSGYRDLE RVDSNSPRGG 250 260 270 280 290 300 MCLVLAEGIA LKAPKIQRYT RNLDEVDWPW LQDLIDGTIG KDEADEGDSA EDANGDDAGE 310 320 330 340 350 360 GADDDGGDEA DEQAGPPRVE PADKYLRDLI AGRPVFSHPS KSGGFRLRYG RSRNHGFATA 370 380 390 400 410 420 GVHPATMHLV DDFLATGTQI KTERPGKAAG VVPVDTIEGP TVRLANGDVR RIDDAEEALA 430 440 450 460 470 480 VRNGVEKILD LGEYLVNYGE FVENNHPLAP ASYTVEWWEQ DLAAAGADVQ AMQDSPHIDL 490 500 510 520 530 540 ADPSAEEAIE WATEYDAPLH PKYTYLWHDV SIEQVCALAD AVEDAQVAQA DGAYADPEMD 550 560 570 580 590 600 GTAGDAHSDD GALVLPRSDA VQQTLEHLLI GHTQDEETIT VTDWVPLVRT LGFSRSLERD 610 620 630 640 650 660 WTREDLSEHA RTYGESESLD AIGVAEDAER EDGQNAIKAI NEVAPFQVRE RAPTRIGNRM 670 680 690 700 710 720 GRPEKSERRD LSPAVHTLSP IGEAGGAQRD VAKATKHADD MSDTPGQVEV EVARRRCPDC 730 740 750 760 770 780 GTETHQANCA ECSGTTEPVY VCPDCEAEVE RDESGRAECG RCETLASPTQ YKVLDLQEAY 790 800 810 820 830 840 RDALQNVGER ETAFEQLKAV KGLTSEEKVP EPMEKGILRA KHDVSAFKDG TVRYDMTDLP 850 860 870 880 890 900 VTAVRASELD VSAERLRGLG YTEDIHGDPL THEDQLVELK VQDIVLSDGA AEHMLQTARF 910 920 930 940 950 960 VDDLLEQYYG LERFYEFDDR EDLVGELVFG MAPHTSAATV GRVVGFTSAA VGYAHPYFHA 970 980 990 1000 1010 1020 AKRRNCFHPD TRLWYEDEND DWEYGTIEEL VESRLDDPQE DDFGTLVQEL DGDLTVSSLG 1030 1040 1050 1060 1070 1080 ENGPCRQPVD AVSKHPAPDH LVEVAVGDRT LRVTPDHTML RAGPDGIEEV PASDLAAGDD 1090 1100 1110 1120 1130 1140 LPAYDGGETT TMTARGEAST AATDGAAPTD TVEAVEYVES DVDHVYCLTV ADTHRVAVEG 1150 1160 1170 1180 1190 1200 TYVGQCDGDE DCVMLLMDGL LNFSKSYLPN QRGGQMDAPL VMSSRIDPSE IDDEAHNMDI 1210 1220 1230 1240 1250 1260 MDAYPREFYE ATREMKDPTE VEDVMKIAEE TLGTDREYTE FRHTHDTANI AAGPDLSAYK 1270 1280 1290 1300 1310 1320 TLGSMEDKMD AQLEISRKLR AVVESDVAER IIEYHFLPDL IGNLRAFSRQ EVRCLDCGES 1330 1340 1350 1360 1370 1380 FRRAPLTGDC RECGGRVNLT VHEGSVNKYI DTAIRVADEF GARDYTKQRL KILERKIESV 1390 FENDHNKQSG IADFM

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY596297 Genomic DNA. Translation: AAV47463.1.
RefSeq	YP_137169.1. NC_006396.1.
3D structure databases
ProteinModelPortal	Q5UZ40.
ModBase	Search...
Protein family/group databases
MEROPS	N10.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3128603.
GenomeReviews	Gene locus rrnAC2691 in contig AY596297_GR.
KEGG	hma:rrnAC2691.
NMPDR	fig|272569.1.peg.2451.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK14714.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC2691-MONOMER.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR006141. Intein_splice_site. IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01445. Intein_Nterm. 1 hit. TIGR00354. PolC. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. False negative. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DP2L_HALMA

Accession

Primary (citable) accession number: Q5UZ40

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	December 7, 2004
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program