Q5UYF6 (ENDA_HALMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	rrnAC2964

Organism

Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

272569 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula › Haloarcula marismortui

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01834
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	tRNA-splicing endonuclease HAMAP-Rule MF_01834		PRO_0000309815
Sites
	Active site	269	1	By similarity
	Active site	280	1	By similarity
	Active site	311	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UYF6 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 3D5E96FED66ABB76	FASTA	335	36,748
	10 20 30 40 50 60 MELTLEGDVV RAGYEARERF YDSRGYGKVR NGDIDLAPVE AAHLLYRGDI ESIDGMDFRG 70 80 90 100 110 120 FLGSAAVSEV DFAVYKDLRD RGFYLTPARE GWVDDAAGAD FVVYPRGKGP WDNEVAYRVR 130 140 150 160 170 180 IIGERDTAVA TDLGGCVLAV VDEESEVTYL DTDRREVSGT SDAAVPATAG ELLGERVVCW 190 200 210 220 230 240 EPPAELYEQA FYGQQLGDDG AVQLSLVEAA YLTQEGMLTV EGGADAVVER GREVEGDRFD 250 260 270 280 290 300 RRLAVYTALR ASGVAPKTGF KFGADFRTYA DVESAENLGH SELLVRVLPA DHRFEPRDLA 310 320 330 LDVRLAHGVR KTMVFALTTD GGDEIEWVAV ERLTP

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY596297 Genomic DNA. Translation: AAV47697.1.
RefSeq	YP_137403.1. NC_006396.1.
3D structure databases
HSSP	HSSP built from PDB template 1R0V based on UniProtKB O29362.
ProteinModelPortal	Q5UYF6.
ModBase	Search...
Protein-protein interaction databases
STRING	272569.rrnAC2964.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV47697; AAV47697; rrnAC2964.
GeneID	3128757.
KEGG	hma:rrnAC2964.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000226561.
KO	K01170.
OMA	FELKYIV.
ProtClustDB	PRK09300.
Enzyme and pathway databases
BioCyc	HMAR272569:GJDH-2658-MONOMER.
Family and domain databases
Gene3D	3.40.1350.10. 2 hits.
HAMAP	MF_01834. EndA_long.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR006676. tRNA_splic. IPR023516. tRNA_splic_arch_long. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 2 hits. PF02778. tRNA_int_endo_N. 2 hits. [Graphical view]
SUPFAM	SSF53032. tRNA_int_endo_C. 2 hits. SSF55267. tRNA_int_endo_N. 2 hits.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENDA_HALMA

Accession

Primary (citable) accession number: Q5UYF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	December 7, 2004
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program