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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei269UniRule annotation1
Active sitei280UniRule annotation1
Active sitei311UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:rrnAC2964
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHaloarculaceaeHaloarcula
Proteomesi
  • UP000001169 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003098151 – 335tRNA-splicing endonucleaseAdd BLAST335

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272569.rrnAC2964

Structurei

3D structure databases

ProteinModelPortaliQ5UYF6
SMRiQ5UYF6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
HOGENOMiHOG000226561
KOiK01170
OMAiGADFRTY
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01834 EndA_long, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR023516 tRNA_splic_arch_long
PANTHERiPTHR21227 PTHR21227, 1 hit
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 2 hits
SUPFAMiSSF53032 SSF53032, 2 hits
SSF55267 SSF55267, 2 hits
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

Q5UYF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELTLEGDVV RAGYEARERF YDSRGYGKVR NGDIDLAPVE AAHLLYRGDI
60 70 80 90 100
ESIDGMDFRG FLGSAAVSEV DFAVYKDLRD RGFYLTPARE GWVDDAAGAD
110 120 130 140 150
FVVYPRGKGP WDNEVAYRVR IIGERDTAVA TDLGGCVLAV VDEESEVTYL
160 170 180 190 200
DTDRREVSGT SDAAVPATAG ELLGERVVCW EPPAELYEQA FYGQQLGDDG
210 220 230 240 250
AVQLSLVEAA YLTQEGMLTV EGGADAVVER GREVEGDRFD RRLAVYTALR
260 270 280 290 300
ASGVAPKTGF KFGADFRTYA DVESAENLGH SELLVRVLPA DHRFEPRDLA
310 320 330
LDVRLAHGVR KTMVFALTTD GGDEIEWVAV ERLTP
Length:335
Mass (Da):36,748
Last modified:December 7, 2004 - v1
Checksum:i3D5E96FED66ABB76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY596297 Genomic DNA Translation: AAV47697.1
RefSeqiWP_004960627.1, NC_006396.1

Genome annotation databases

EnsemblBacteriaiAAV47697; AAV47697; rrnAC2964
GeneIDi3128757
KEGGihma:rrnAC2964
PATRICifig|272569.17.peg.3524

Similar proteinsi

Entry informationi

Entry nameiENDA_HALMA
AccessioniPrimary (citable) accession number: Q5UYF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 7, 2004
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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