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Q5UYF6

- ENDA_HALMA

UniProt

Q5UYF6 - ENDA_HALMA

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Protein
tRNA-splicing endonuclease
Gene
endA, rrnAC2964
Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei269 – 2691 By similarity
Active sitei280 – 2801 By similarity
Active sitei311 – 3111 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-2658-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease
Gene namesi
Name:endA
Ordered Locus Names:rrnAC2964
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
ProteomesiUP000001169: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335tRNA-splicing endonucleaseUniRule annotation
PRO_0000309815Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272569.rrnAC2964.

Structurei

3D structure databases

ProteinModelPortaliQ5UYF6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000226561.
KOiK01170.
OMAiRGFYLSP.

Family and domain databases

Gene3Di3.40.1350.10. 2 hits.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5UYF6-1 [UniParc]FASTAAdd to Basket

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MELTLEGDVV RAGYEARERF YDSRGYGKVR NGDIDLAPVE AAHLLYRGDI    50
ESIDGMDFRG FLGSAAVSEV DFAVYKDLRD RGFYLTPARE GWVDDAAGAD 100
FVVYPRGKGP WDNEVAYRVR IIGERDTAVA TDLGGCVLAV VDEESEVTYL 150
DTDRREVSGT SDAAVPATAG ELLGERVVCW EPPAELYEQA FYGQQLGDDG 200
AVQLSLVEAA YLTQEGMLTV EGGADAVVER GREVEGDRFD RRLAVYTALR 250
ASGVAPKTGF KFGADFRTYA DVESAENLGH SELLVRVLPA DHRFEPRDLA 300
LDVRLAHGVR KTMVFALTTD GGDEIEWVAV ERLTP 335
Length:335
Mass (Da):36,748
Last modified:December 7, 2004 - v1
Checksum:i3D5E96FED66ABB76
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY596297 Genomic DNA. Translation: AAV47697.1.
RefSeqiYP_137403.1. NC_006396.1.

Genome annotation databases

EnsemblBacteriaiAAV47697; AAV47697; rrnAC2964.
GeneIDi3128757.
KEGGihma:rrnAC2964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY596297 Genomic DNA. Translation: AAV47697.1 .
RefSeqi YP_137403.1. NC_006396.1.

3D structure databases

ProteinModelPortali Q5UYF6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272569.rrnAC2964.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV47697 ; AAV47697 ; rrnAC2964 .
GeneIDi 3128757.
KEGGi hma:rrnAC2964.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000226561.
KOi K01170.
OMAi RGFYLSP.

Enzyme and pathway databases

BioCyci HMAR272569:GJDH-2658-MONOMER.

Family and domain databases

Gene3Di 3.40.1350.10. 2 hits.
HAMAPi MF_01834. EndA_long.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Entry informationi

Entry nameiENDA_HALMA
AccessioniPrimary (citable) accession number: Q5UYF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 7, 2004
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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