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Q5UYF6

- ENDA_HALMA

UniProt

Q5UYF6 - ENDA_HALMA

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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei269 – 2691UniRule annotation
Active sitei280 – 2801UniRule annotation
Active sitei311 – 3111UniRule annotation

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-2658-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:rrnAC2964
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
ProteomesiUP000001169: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335tRNA-splicing endonucleasePRO_0000309815Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272569.rrnAC2964.

Structurei

3D structure databases

ProteinModelPortaliQ5UYF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000226561.
KOiK01170.
OMAiRGFYLSP.

Family and domain databases

Gene3Di3.40.1350.10. 2 hits.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5UYF6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELTLEGDVV RAGYEARERF YDSRGYGKVR NGDIDLAPVE AAHLLYRGDI
60 70 80 90 100
ESIDGMDFRG FLGSAAVSEV DFAVYKDLRD RGFYLTPARE GWVDDAAGAD
110 120 130 140 150
FVVYPRGKGP WDNEVAYRVR IIGERDTAVA TDLGGCVLAV VDEESEVTYL
160 170 180 190 200
DTDRREVSGT SDAAVPATAG ELLGERVVCW EPPAELYEQA FYGQQLGDDG
210 220 230 240 250
AVQLSLVEAA YLTQEGMLTV EGGADAVVER GREVEGDRFD RRLAVYTALR
260 270 280 290 300
ASGVAPKTGF KFGADFRTYA DVESAENLGH SELLVRVLPA DHRFEPRDLA
310 320 330
LDVRLAHGVR KTMVFALTTD GGDEIEWVAV ERLTP
Length:335
Mass (Da):36,748
Last modified:December 7, 2004 - v1
Checksum:i3D5E96FED66ABB76
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY596297 Genomic DNA. Translation: AAV47697.1.
RefSeqiYP_137403.1. NC_006396.1.

Genome annotation databases

EnsemblBacteriaiAAV47697; AAV47697; rrnAC2964.
GeneIDi3128757.
KEGGihma:rrnAC2964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY596297 Genomic DNA. Translation: AAV47697.1 .
RefSeqi YP_137403.1. NC_006396.1.

3D structure databases

ProteinModelPortali Q5UYF6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272569.rrnAC2964.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV47697 ; AAV47697 ; rrnAC2964 .
GeneIDi 3128757.
KEGGi hma:rrnAC2964.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000226561.
KOi K01170.
OMAi RGFYLSP.

Enzyme and pathway databases

BioCyci HMAR272569:GJDH-2658-MONOMER.

Family and domain databases

Gene3Di 3.40.1350.10. 2 hits.
HAMAPi MF_01834. EndA_long.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Entry informationi

Entry nameiENDA_HALMA
AccessioniPrimary (citable) accession number: Q5UYF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 7, 2004
Last modified: October 1, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3