Q5UY62 (RIFK_HALMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	rrnAC3074

Organism

Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

272569 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Riboflavin kinase HAMAP MF_01285		PRO_0000322084
Regions
	Nucleotide binding	196 – 201	6	CDP By similarity
	Nucleotide binding	300 – 303	4	CDP By similarity
	Region	1 – 186	186	H-T-H motif-like HAMAP MF_01285
	Region	187 – 320	134	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	225	1	Magnesium By similarity
	Metal binding	227	1	Magnesium By similarity
	Binding site	287	1	FMN By similarity
	Binding site	295	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UY62 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 5C62619A06C09426	FASTA	320	35,682
	10 20 30 40 50 60 MRTRGSRNWR SRASSGSGTR SWSIEATDRN LSQQCDVAEH VRQRVKLYPA QTVKRAFRTN 70 80 90 100 110 120 PVSVSLRSKS GMHSTFGMLI RRESLLQRMA ESTGQGVGRD ELATLKLLAL DGALDESTKV 130 140 150 160 170 180 SCADLAERLD ASNQTASRRL QRLEDAGLLA RDIVSDGQEV ELTGDGERRL QSEYADYRRI 190 200 210 220 230 240 FESDASVDLT GVVTSGMGEG RHYITLPGYM EQFIERLGYE PFAGTLNLEL TAESVRKRAR 250 260 270 280 290 300 MSAIEPVTIE GWEDDERTYG PAYCYPASIE GSDSEYEPAH VIAPERTHHG EEQLEVIAPE 310 320 KLREVLELAD GDEVIVHVSE

References

[1]

"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY596297 Genomic DNA. Translation: AAV47791.1.
RefSeq	YP_137497.1. NC_006396.1.
3D structure databases
ProteinModelPortal	Q5UY62.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3129198.
GenomeReviews	Gene locus rrnAC3074 in contig AY596297_GR.
KEGG	hma:rrnAC3074.
NMPDR	fig|272569.1.peg.2787.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553349.
ProtClustDB	CLSK511620.
Enzyme and pathway databases
BioCyc	HMAR272569:RRNAC3074-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_HALMA

Accession

Primary (citable) accession number: Q5UY62

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 7, 2004
Last modified:	November 16, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program