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Protein

Varv peptide A/Kalata-B1

Gene

Vok1

Organism
Viola odorata (Sweet violet)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably participates in a plant defense mechanism. Has hemolytic activity.PROSITE-ProRule annotationCurated1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cytolysis, Hemolysis, Plant defense

Protein family/group databases

TCDBi9.B.48.1.3. the cyclotide (cyclotide) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Varv peptide A/Kalata-B1
Alternative name(s):
Cyclotide k1
Cleaved into the following 2 chains:
Gene namesi
Name:Vok11 Publication
OrganismiViola odorata (Sweet violet)
Taxonomic identifieri97441 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesViolaceaeViola

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 66461 PublicationPRO_0000294967Add
BLAST
Peptidei67 – 9529Varv peptide APROSITE-ProRule annotation1 PublicationPRO_0000294968Add
BLAST
Propeptidei96 – 120251 PublicationPRO_0000294969Add
BLAST
Peptidei121 – 14929Kalata-B1PROSITE-ProRule annotation1 PublicationPRO_0000294970Add
BLAST
Propeptidei150 – 174251 PublicationPRO_0000294971Add
BLAST
Peptidei175 – 20329Varv peptide APROSITE-ProRule annotation1 PublicationPRO_0000294972Add
BLAST
Propeptidei204 – 20741 PublicationPRO_0000294973

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki67 ↔ 95Cyclopeptide (Gly-Asn)1 Publication
Disulfide bondi71 ↔ 85PROSITE-ProRule annotationBy similarity
Disulfide bondi75 ↔ 87PROSITE-ProRule annotationBy similarity
Disulfide bondi80 ↔ 92PROSITE-ProRule annotationBy similarity
Cross-linki121 ↔ 149Cyclopeptide (Gly-Asn)1 Publication
Disulfide bondi125 ↔ 139PROSITE-ProRule annotationBy similarity
Disulfide bondi129 ↔ 141PROSITE-ProRule annotationBy similarity
Disulfide bondi134 ↔ 146PROSITE-ProRule annotationBy similarity
Cross-linki175 ↔ 203Cyclopeptide (Gly-Asn)1 Publication
Disulfide bondi179 ↔ 193PROSITE-ProRule annotationBy similarity
Disulfide bondi183 ↔ 195PROSITE-ProRule annotationBy similarity
Disulfide bondi188 ↔ 200PROSITE-ProRule annotationBy similarity

Post-translational modificationi

Varv peptide A and kalata-B1 are cyclic peptides.1 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
207
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi158 – 17114Combined sources
Beta strandi172 – 1743Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WN4NMR-A150-177[»]
ProteinModelPortaliQ5USN7.
SMRiQ5USN7. Positions 67-94, 121-148, 150-177, 179-206.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5USN7.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

Sequence similaritiesi

Belongs to the cyclotide family. Moebius subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Knottin, Repeat, Signal

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012324. Cyclotide_moebius_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 3 hits.
[Graphical view]
SUPFAMiSSF57038. SSF57038. 3 hits.
PROSITEiPS51052. CYCLOTIDE. 3 hits.
PS60009. CYCLOTIDE_MOEBIUS. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5USN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMFIVLVLS AAFALPAAFA TEQDVITLQA YEELLKNGAA NGMTKTVISS
60 70 80 90 100
PVLEEALVSY SKNKLGGLPV CGETCVGGTC NTPGCSCSWP VCTRNSLEST
110 120 130 140 150
KSANPLLEEA LTAFAKKGLG GLPVCGETCV GGTCNTPGCT CSWPVCTRNA
160 170 180 190 200
LETQKPNHLL EEALVAFAKK GNLGGLPVCG ETCVGGTCNT PGCSCSWPVC

TRNALAM
Length:207
Mass (Da):21,334
Last modified:December 7, 2004 - v1
Checksum:i960241C55F696F72
GO

Mass spectrometryi

Molecular mass is 2878.2 Da from positions 67 - 95. Determined by MALDI. 1 Publication
Molecular mass is 2890.2 Da from positions 121 - 149. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY630566 mRNA. Translation: AAU04395.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY630566 mRNA. Translation: AAU04395.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WN4NMR-A150-177[»]
ProteinModelPortaliQ5USN7.
SMRiQ5USN7. Positions 67-94, 121-148, 150-177, 179-206.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi9.B.48.1.3. the cyclotide (cyclotide) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ5USN7.

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012324. Cyclotide_moebius_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 3 hits.
[Graphical view]
SUPFAMiSSF57038. SSF57038. 3 hits.
PROSITEiPS51052. CYCLOTIDE. 3 hits.
PS60009. CYCLOTIDE_MOEBIUS. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVARA_VIOOD
AccessioniPrimary (citable) accession number: Q5USN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: December 7, 2004
Last modified: December 9, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.