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Reviewed, UniProtKB/Swiss-Prot Q5UQX1 (MCE_MIMIV)

Last modified November 25, 2008. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable mRNA-capping enzyme
Including the following 3 domains:
    1- Recommended name:
            Polynucleotide 5'-triphosphatase
              EC=3.1.3.33
        Alternative name(s):
            mRNA 5'-triphosphatase
              Short name=TPase
    2- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.50
        Alternative name(s):
            GTP--RNA guanylyltransferase
              Short name=GTase
    3- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
Gene names
Ordered Locus Names: MIMI_R382
OrganismAcanthamoeba polyphaga mimivirus (APMV) [Complete proteome]
Taxonomic identifier212035 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus
Virus hostAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]

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Protein attributes

Sequence length1170 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Responsible for methylating the 5'-cap structure of mRNAs.

Catalytic activity

A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate.

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.

Pathway

mRNA processing; mRNA capping.

Subcellular location

Virion.

Sequence similarities

Belongs to the viral GTase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11701170Probable mRNA-capping enzyme
PRO_0000210133

Sites

Active site2921N6-GMP-lysine intermediate Potential

Secondary structure

1 ............................... 1170
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q5UQX1-1 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 320D8F4137C55AD6

FASTA1,170136,508
        10         20         30         40         50         60 
MGTKLKKSNN DITIFSENEY NEIVEMLRDY SNGDNLEFEV SFKNINYPNF MRITEHYINI 

        70         80         90        100        110        120 
TPENKIESNN YLDISLIFPD KNVYRVSLFN QEQIGEFITK FSKASSNDIS RYIVSLDPSD 

       130        140        150        160        170        180 
DIEIVYKNRG SGKLIGIDNW AITIKSTEEI PLVAGKSKIS KPKITGSERI MYRYKTRYSF 

       190        200        210        220        230        240 
TINKNSRIDI TDVKSSPIIW KLMTVPSNYE LELELINKID INTLESELLN VFMIIQDTKI 

       250        260        270        280        290        300 
PISKAESDTV VEEYRNLLNV RQTNNLDSRN VISVNSNHII NFIPNRYAVT DKADGERYFL 

       310        320        330        340        350        360 
FSLNSGIYLL SINLTVKKLN IPVLEKRYQN MLIDGEYIKT TGHDLFMVFD VIFAEGTDYR 

       370        380        390        400        410        420 
YDNTYSLPKR IIIINNIIDK CFGNLIPFND YTDKHNNLEL DSIKTYYKSE LSNYWKNFKN 

       430        440        450        460        470        480 
RLNKSTDLFI TRKLYLVPYG IDSSEIFMYA DMIWKLYVYN ELTPYQLDGI IYTPINSPYL 

       490        500        510        520        530        540 
IRGGIDAYDT IPMEYKWKPP SQNSIDFYIR FKKDVSGADA VYYDNSVERA EGKPYKICLL 

       550        560        570        580        590        600 
YVGLNKQGQE IPIQFKVNGV EQTANIYTKD GEATDINGNA INDNTVVEFV FDTLKIDMDD 

       610        620        630        640        650        660 
SYKWIPIRTR YDKTESVQKY HKRYGNNLQI ANRIWKTITN PITEDIISSL GDPTTFNKEI 

       670        680        690        700        710        720 
TLLSDFRDTK YNKQALTYYQ KNTSNAAGMR AFNNWIKSNM ITTYCRDGSK VLDIGCGRGG 

       730        740        750        760        770        780 
DLIKFINAGV EFYVGIDIDN NGLYVINDSA NNRYKNLKKT IQNIPPMYFI NADARGLFTL 

       790        800        810        820        830        840 
EAQEKILPGM PDFNKSLINK YLVGNKYDTI NCQFTIHYYL SDELSWNNFC KNINNQLKDN 

       850        860        870        880        890        900 
GYLLITSFDG NLIHNKLKGK QKLSSSYTDN RGNKNIFFEI NKIYSDTDKV GLGMAIDLYN 

       910        920        930        940        950        960 
SLISNPGTYI REYLVFPEFL EKSLKEKCGL ELVESDLFYN IFNTYKNYFK KTYNEYGMTD 

       970        980        990       1000       1010       1020 
VSSKKHSEIR EFYLSLEGNA NNDIEIDIAR ASFKLAMLNR YYVFRKTSTI NITEPSRIVN 

      1030       1040       1050       1060       1070       1080 
ELNNRIDLGK FIMPYFRTNN MFIDLDNVDT DINRVYRNIR NKYRTTRPHV YLIKHNINEN 

      1090       1100       1110       1120       1130       1140 
RLEDIYLSNN KLDFSKIKNG SDPKVLLIYK SPDKQFYPLY YQNYQSMPFD LDQIYLPDKK 

      1150       1160       1170 
KYLLDSDRII NDLNILINLT EKIKNIPQLS

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References

[1]"The 1.2-megabase genome sequence of Mimivirus."
Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M.
Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rowbotham-Bradford.
[2]"Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products."
Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.
J. Virol. 80:11678-11685(2006) [PubMed: 16971431] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AY653733 Genomic DNA. Translation: AAV50651.1.
RefSeqYP_142736.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2QY2X-ray2.00A/B1-237[»]
2QZEX-ray2.90A/B1-237[»]
3BGYX-ray1.65A/B1-237[»]
ModBaseSearch...

Genome annotation databases

GeneID3162607.

Family and domain databases

InterProIPR001339. mRNA_cap_enzyme.
IPR004971. Pox_MCEL.
[Graphical view]
PfamPF01331. mRNA_cap_enzyme. 1 hit.
PF03291. Pox_MCEL. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMCE_MIMIV
AccessionPrimary (citable) accession number: Q5UQX1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents