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Q5UQR0

- DPOL_MIMIV

UniProt

Q5UQR0 - DPOL_MIMIV

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Protein

DNA polymerase

Gene

POLB

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: InterPro
  4. nucleotide binding Source: InterPro

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Cleaved into the following chain:
Gene namesi
Name:POLB
Ordered Locus Names:MIMI_R322
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10521052DNA polymerase, 1st partSequence AnalysisPRO_0000041777Add
BLAST
Chaini1053 – 1403351Mimv polB inteinSequence AnalysisPRO_0000041778Add
BLAST
Chaini1404 – 1740337DNA polymerase, 2nd partSequence AnalysisPRO_0000041779Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliQ5UQR0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1189 – 1334146DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated
Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.420.10. 3 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 2 hits.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF53098. SSF53098. 3 hits.
SSF55608. SSF55608. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5UQR0-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MPSETIDSTK QFEFQISDWN SYHELDQEEE EKYVIQLFGR TEDDHDVCLK
60 70 80 90 100
VTGYTPFFYV EIPKQWKQRQ VDKFVEILKN KVQYHCKKNL DEDFDLSKSL
110 120 130 140 150
IKYAMVKKHK FYNFRNKQLY NFLLLVFKSH TAMKEFSSIL ARPLEAKGLT
160 170 180 190 200
NKPMLYQRYE SNIEPHIRFM HINNLSSCGW ASIDKDKLKK IPEYSNCDYS
210 220 230 240 250
FSVNWKDVKP SNNDDRMAPF KIMGYDIECV SCDQNFPQAE RPSDKIIQIG
260 270 280 290 300
ITMYRYGSMK CYEQHILTLK KCAPIEGVNV ECYKKEKGLL RGFAKKIAEL
310 320 330 340 350
RPDFKTGYNN FGFDDKYIYD RILRIDKREG KKQGVNINAL KNKFMDEILR
360 370 380 390 400
TIGKVNNNYL IENEGLDRIP IYTTVKDKKI SSKASRFIQI RGGTYVENGN
410 420 430 440 450
NLKYVQSPGI TYFEVKNLSS SALGDNELKF IQIPGVLSID MMKVIQRDHR
460 470 480 490 500
LIGYKLDNVS ANFITEKADK IIEMPHNQED SDSEKEDEDT DDKTYDVNIY
510 520 530 540 550
TKSTKALEKD SYIQIMVNDG YSSSPLSEGA KYKVYDIQTI TEKKLNEKTN
560 570 580 590 600
KEEIFVYQAI KTKICQKDIQ QLRETIKNPL LGISWTFAKD DMHHTKINEY
610 620 630 640 650
FEEGDPKKIR QIAKYCLKDC KLVNLLLAKL EIIVNSVGMA KVCHVPLSYL
660 670 680 690 700
FLRGQGVKIF SLVSKKCREK NFLIPVLRRK SKDNEGDEDE TYEGATVITP
710 720 730 740 750
KPNVYLSPIG VLDYSSLYPN SMRERNLSQE CYVDDSKYDN LPGYIYHDVE
760 770 780 790 800
IILKDKKGKI LRNIDGTPQK EYHRFAQEII TDEQINRELK DIFDKINTVF
810 820 830 840 850
ENNVAIIQNQ KYFTEKNISE LIDKHKNISD SKIEDIEFDE SLSDKRKNKL
860 870 880 890 900
VDAEKDSLDK NIGFYQKIKS QIDKIKLDSK IEIDNLSKNL NEEEKSKQIN
910 920 930 940 950
KMELNTKNLI SKVFSKYLIT EQQREELIVL EKERAKRSVN AEKAKVYNTV
960 970 980 990 1000
DGITVRYGIL PEILTELLNK RKETNGKLAN EKDPFVKAIL NALQLAFKVT
1010 1020 1030 1040 1050
ANSLYGQTGA PTSPLYFIAI AACTTAIGRE RLHYAKKTVE DNFPGSEVIY
1060 1070 1080 1090 1100
GDSVTGDTPI ITRHQNGDIN ITTIEELGSK WKPYEIFKAH EKNSNRKFKQ
1110 1120 1130 1140 1150
QSQYPTDSEV WTAKGWAKIK RVIRHKTVKK IYRVLTHTGC IDVTEDHSLL
1160 1170 1180 1190 1200
DPNQNIIKPI NCQIGTELLH GFPESNNVYD NISEQEAYVW GFFMGDGSCG
1210 1220 1230 1240 1250
SYQTKNGIKY SWALNNQDLD VLNKCKKYLE ETENIQFKIL DTMKSSSVYK
1260 1270 1280 1290 1300
LVPIRKIKYM VNKYRKIFYD NKKYKLVPKE ILNSTKDIKN SFLEGYYAAD
1310 1320 1330 1340 1350
GSRKETENMG CRRCDIKGKI SAQCLFYLLK SLGYNVSINI RSDKNQIYRL
1360 1370 1380 1390 1400
TFSNKKQRKN PIAIKKIQLM NETSNDHDGD YVYDLETESG SFHAGVGEMI
1410 1420 1430 1440 1450
VKNTDSIFIN FHIKDENGEE KTDKEALMKT IAKCQRAAKL INQNVPKPQS
1460 1470 1480 1490 1500
IVYEKTLHPF ILVAKKKYVG LLFEKSPDKY FLKSMGIVLK RRDNAPIVKI
1510 1520 1530 1540 1550
VVGGIIDNIL KNRDIDKAIE YTKIVLDKLM NGEYPMDKFI ISKTLKSRYK
1560 1570 1580 1590 1600
KPSTIAHKVL ADRMAVRDPG NKPQINDRIP FVYIVKDMGK KKKKDILQGD
1610 1620 1630 1640 1650
LIEHPEYVIA NNLKIDYLYY LEHQIINPAS QILELMMDTK DVQKFFNKYI
1660 1670 1680 1690 1700
IDEQNKRKGA QSLTKWMDFS KLPKESGSKT AKKPYQSQKL QKTKSSNKSQ
1710 1720 1730 1740
IDPKYINLIK NKSRKHECQN MNKWISSTDK CTDDWEPIVE
Length:1,740
Mass (Da):201,945
Last modified:December 7, 2004 - v1
Checksum:iDC8B2AFF4EEE7724
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY606804 Genomic DNA. Translation: AAU11330.1.
AY653733 Genomic DNA. Translation: AAV50591.1.
RefSeqiYP_003986825.1. NC_014649.1.

Genome annotation databases

GeneIDi9924939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY606804 Genomic DNA. Translation: AAU11330.1 .
AY653733 Genomic DNA. Translation: AAV50591.1 .
RefSeqi YP_003986825.1. NC_014649.1.

3D structure databases

ProteinModelPortali Q5UQR0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9924939.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.420.10. 3 hits.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 2 hits.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view ]
SMARTi SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF53098. SSF53098. 3 hits.
SSF55608. SSF55608. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new example of viral intein in Mimivirus."
    Ogata H., Raoult D., Claverie J.-M.
    Virol. J. 2:8-8(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISCUSSION OF SEQUENCE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.

Entry informationi

Entry nameiDPOL_MIMIV
AccessioniPrimary (citable) accession number: Q5UQR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: October 1, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3