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Q5UQR0

- DPOL_MIMIV

UniProt

Q5UQR0 - DPOL_MIMIV

Protein

DNA polymerase

Gene

POLB

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. endonuclease activity Source: InterPro
    4. nucleotide binding Source: InterPro

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase (EC:2.7.7.7)
    Cleaved into the following chain:
    Gene namesi
    Name:POLB
    Ordered Locus Names:MIMI_R322
    OrganismiAcanthamoeba polyphaga mimivirus (APMV)
    Taxonomic identifieri212035 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
    Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
    ProteomesiUP000001134: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10521052DNA polymerase, 1st partSequence AnalysisPRO_0000041777Add
    BLAST
    Chaini1053 – 1403351Mimv polB inteinSequence AnalysisPRO_0000041778Add
    BLAST
    Chaini1404 – 1740337DNA polymerase, 2nd partSequence AnalysisPRO_0000041779Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Structurei

    3D structure databases

    ProteinModelPortaliQ5UQR0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1189 – 1334146DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Curated
    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.30.420.10. 3 hits.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR028992. Hedgehog/Intein_dom.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 2 hits.
    PF03104. DNA_pol_B_exo1. 1 hit.
    [Graphical view]
    SMARTiSM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 2 hits.
    SSF53098. SSF53098. 3 hits.
    SSF55608. SSF55608. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5UQR0-1 [UniParc]FASTAAdd to Basket

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    MPSETIDSTK QFEFQISDWN SYHELDQEEE EKYVIQLFGR TEDDHDVCLK     50
    VTGYTPFFYV EIPKQWKQRQ VDKFVEILKN KVQYHCKKNL DEDFDLSKSL 100
    IKYAMVKKHK FYNFRNKQLY NFLLLVFKSH TAMKEFSSIL ARPLEAKGLT 150
    NKPMLYQRYE SNIEPHIRFM HINNLSSCGW ASIDKDKLKK IPEYSNCDYS 200
    FSVNWKDVKP SNNDDRMAPF KIMGYDIECV SCDQNFPQAE RPSDKIIQIG 250
    ITMYRYGSMK CYEQHILTLK KCAPIEGVNV ECYKKEKGLL RGFAKKIAEL 300
    RPDFKTGYNN FGFDDKYIYD RILRIDKREG KKQGVNINAL KNKFMDEILR 350
    TIGKVNNNYL IENEGLDRIP IYTTVKDKKI SSKASRFIQI RGGTYVENGN 400
    NLKYVQSPGI TYFEVKNLSS SALGDNELKF IQIPGVLSID MMKVIQRDHR 450
    LIGYKLDNVS ANFITEKADK IIEMPHNQED SDSEKEDEDT DDKTYDVNIY 500
    TKSTKALEKD SYIQIMVNDG YSSSPLSEGA KYKVYDIQTI TEKKLNEKTN 550
    KEEIFVYQAI KTKICQKDIQ QLRETIKNPL LGISWTFAKD DMHHTKINEY 600
    FEEGDPKKIR QIAKYCLKDC KLVNLLLAKL EIIVNSVGMA KVCHVPLSYL 650
    FLRGQGVKIF SLVSKKCREK NFLIPVLRRK SKDNEGDEDE TYEGATVITP 700
    KPNVYLSPIG VLDYSSLYPN SMRERNLSQE CYVDDSKYDN LPGYIYHDVE 750
    IILKDKKGKI LRNIDGTPQK EYHRFAQEII TDEQINRELK DIFDKINTVF 800
    ENNVAIIQNQ KYFTEKNISE LIDKHKNISD SKIEDIEFDE SLSDKRKNKL 850
    VDAEKDSLDK NIGFYQKIKS QIDKIKLDSK IEIDNLSKNL NEEEKSKQIN 900
    KMELNTKNLI SKVFSKYLIT EQQREELIVL EKERAKRSVN AEKAKVYNTV 950
    DGITVRYGIL PEILTELLNK RKETNGKLAN EKDPFVKAIL NALQLAFKVT 1000
    ANSLYGQTGA PTSPLYFIAI AACTTAIGRE RLHYAKKTVE DNFPGSEVIY 1050
    GDSVTGDTPI ITRHQNGDIN ITTIEELGSK WKPYEIFKAH EKNSNRKFKQ 1100
    QSQYPTDSEV WTAKGWAKIK RVIRHKTVKK IYRVLTHTGC IDVTEDHSLL 1150
    DPNQNIIKPI NCQIGTELLH GFPESNNVYD NISEQEAYVW GFFMGDGSCG 1200
    SYQTKNGIKY SWALNNQDLD VLNKCKKYLE ETENIQFKIL DTMKSSSVYK 1250
    LVPIRKIKYM VNKYRKIFYD NKKYKLVPKE ILNSTKDIKN SFLEGYYAAD 1300
    GSRKETENMG CRRCDIKGKI SAQCLFYLLK SLGYNVSINI RSDKNQIYRL 1350
    TFSNKKQRKN PIAIKKIQLM NETSNDHDGD YVYDLETESG SFHAGVGEMI 1400
    VKNTDSIFIN FHIKDENGEE KTDKEALMKT IAKCQRAAKL INQNVPKPQS 1450
    IVYEKTLHPF ILVAKKKYVG LLFEKSPDKY FLKSMGIVLK RRDNAPIVKI 1500
    VVGGIIDNIL KNRDIDKAIE YTKIVLDKLM NGEYPMDKFI ISKTLKSRYK 1550
    KPSTIAHKVL ADRMAVRDPG NKPQINDRIP FVYIVKDMGK KKKKDILQGD 1600
    LIEHPEYVIA NNLKIDYLYY LEHQIINPAS QILELMMDTK DVQKFFNKYI 1650
    IDEQNKRKGA QSLTKWMDFS KLPKESGSKT AKKPYQSQKL QKTKSSNKSQ 1700
    IDPKYINLIK NKSRKHECQN MNKWISSTDK CTDDWEPIVE 1740
    Length:1,740
    Mass (Da):201,945
    Last modified:December 7, 2004 - v1
    Checksum:iDC8B2AFF4EEE7724
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY606804 Genomic DNA. Translation: AAU11330.1.
    AY653733 Genomic DNA. Translation: AAV50591.1.
    RefSeqiYP_003986825.1. NC_014649.1.

    Genome annotation databases

    GeneIDi9924939.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY606804 Genomic DNA. Translation: AAU11330.1 .
    AY653733 Genomic DNA. Translation: AAV50591.1 .
    RefSeqi YP_003986825.1. NC_014649.1.

    3D structure databases

    ProteinModelPortali Q5UQR0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 9924939.

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.30.420.10. 3 hits.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR028992. Hedgehog/Intein_dom.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 2 hits.
    PF03104. DNA_pol_B_exo1. 1 hit.
    [Graphical view ]
    SMARTi SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 2 hits.
    SSF53098. SSF53098. 3 hits.
    SSF55608. SSF55608. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new example of viral intein in Mimivirus."
      Ogata H., Raoult D., Claverie J.-M.
      Virol. J. 2:8-8(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISCUSSION OF SEQUENCE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Rowbotham-Bradford.

    Entry informationi

    Entry nameiDPOL_MIMIV
    AccessioniPrimary (citable) accession number: Q5UQR0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3