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Q5UQR0 (DPOL_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase

EC=2.7.7.7

Cleaved into the following chain:

  1. Mimv polB intein
Gene names
Name:POLB
Ordered Locus Names:MIMI_R322
OrganismAcanthamoeba polyphaga mimivirus (APMV) [Reference proteome]
Taxonomic identifier212035 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length1740 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10521052DNA polymerase, 1st part Potential
PRO_0000041777
Chain1053 – 1403351Mimv polB intein Potential
PRO_0000041778
Chain1404 – 1740337DNA polymerase, 2nd part Potential
PRO_0000041779

Regions

Domain1189 – 1334146DOD-type homing endonuclease

Sequences

Sequence LengthMass (Da)Tools
Q5UQR0 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: DC8B2AFF4EEE7724

FASTA1,740201,945
        10         20         30         40         50         60 
MPSETIDSTK QFEFQISDWN SYHELDQEEE EKYVIQLFGR TEDDHDVCLK VTGYTPFFYV 

        70         80         90        100        110        120 
EIPKQWKQRQ VDKFVEILKN KVQYHCKKNL DEDFDLSKSL IKYAMVKKHK FYNFRNKQLY 

       130        140        150        160        170        180 
NFLLLVFKSH TAMKEFSSIL ARPLEAKGLT NKPMLYQRYE SNIEPHIRFM HINNLSSCGW 

       190        200        210        220        230        240 
ASIDKDKLKK IPEYSNCDYS FSVNWKDVKP SNNDDRMAPF KIMGYDIECV SCDQNFPQAE 

       250        260        270        280        290        300 
RPSDKIIQIG ITMYRYGSMK CYEQHILTLK KCAPIEGVNV ECYKKEKGLL RGFAKKIAEL 

       310        320        330        340        350        360 
RPDFKTGYNN FGFDDKYIYD RILRIDKREG KKQGVNINAL KNKFMDEILR TIGKVNNNYL 

       370        380        390        400        410        420 
IENEGLDRIP IYTTVKDKKI SSKASRFIQI RGGTYVENGN NLKYVQSPGI TYFEVKNLSS 

       430        440        450        460        470        480 
SALGDNELKF IQIPGVLSID MMKVIQRDHR LIGYKLDNVS ANFITEKADK IIEMPHNQED 

       490        500        510        520        530        540 
SDSEKEDEDT DDKTYDVNIY TKSTKALEKD SYIQIMVNDG YSSSPLSEGA KYKVYDIQTI 

       550        560        570        580        590        600 
TEKKLNEKTN KEEIFVYQAI KTKICQKDIQ QLRETIKNPL LGISWTFAKD DMHHTKINEY 

       610        620        630        640        650        660 
FEEGDPKKIR QIAKYCLKDC KLVNLLLAKL EIIVNSVGMA KVCHVPLSYL FLRGQGVKIF 

       670        680        690        700        710        720 
SLVSKKCREK NFLIPVLRRK SKDNEGDEDE TYEGATVITP KPNVYLSPIG VLDYSSLYPN 

       730        740        750        760        770        780 
SMRERNLSQE CYVDDSKYDN LPGYIYHDVE IILKDKKGKI LRNIDGTPQK EYHRFAQEII 

       790        800        810        820        830        840 
TDEQINRELK DIFDKINTVF ENNVAIIQNQ KYFTEKNISE LIDKHKNISD SKIEDIEFDE 

       850        860        870        880        890        900 
SLSDKRKNKL VDAEKDSLDK NIGFYQKIKS QIDKIKLDSK IEIDNLSKNL NEEEKSKQIN 

       910        920        930        940        950        960 
KMELNTKNLI SKVFSKYLIT EQQREELIVL EKERAKRSVN AEKAKVYNTV DGITVRYGIL 

       970        980        990       1000       1010       1020 
PEILTELLNK RKETNGKLAN EKDPFVKAIL NALQLAFKVT ANSLYGQTGA PTSPLYFIAI 

      1030       1040       1050       1060       1070       1080 
AACTTAIGRE RLHYAKKTVE DNFPGSEVIY GDSVTGDTPI ITRHQNGDIN ITTIEELGSK 

      1090       1100       1110       1120       1130       1140 
WKPYEIFKAH EKNSNRKFKQ QSQYPTDSEV WTAKGWAKIK RVIRHKTVKK IYRVLTHTGC 

      1150       1160       1170       1180       1190       1200 
IDVTEDHSLL DPNQNIIKPI NCQIGTELLH GFPESNNVYD NISEQEAYVW GFFMGDGSCG 

      1210       1220       1230       1240       1250       1260 
SYQTKNGIKY SWALNNQDLD VLNKCKKYLE ETENIQFKIL DTMKSSSVYK LVPIRKIKYM 

      1270       1280       1290       1300       1310       1320 
VNKYRKIFYD NKKYKLVPKE ILNSTKDIKN SFLEGYYAAD GSRKETENMG CRRCDIKGKI 

      1330       1340       1350       1360       1370       1380 
SAQCLFYLLK SLGYNVSINI RSDKNQIYRL TFSNKKQRKN PIAIKKIQLM NETSNDHDGD 

      1390       1400       1410       1420       1430       1440 
YVYDLETESG SFHAGVGEMI VKNTDSIFIN FHIKDENGEE KTDKEALMKT IAKCQRAAKL 

      1450       1460       1470       1480       1490       1500 
INQNVPKPQS IVYEKTLHPF ILVAKKKYVG LLFEKSPDKY FLKSMGIVLK RRDNAPIVKI 

      1510       1520       1530       1540       1550       1560 
VVGGIIDNIL KNRDIDKAIE YTKIVLDKLM NGEYPMDKFI ISKTLKSRYK KPSTIAHKVL 

      1570       1580       1590       1600       1610       1620 
ADRMAVRDPG NKPQINDRIP FVYIVKDMGK KKKKDILQGD LIEHPEYVIA NNLKIDYLYY 

      1630       1640       1650       1660       1670       1680 
LEHQIINPAS QILELMMDTK DVQKFFNKYI IDEQNKRKGA QSLTKWMDFS KLPKESGSKT 

      1690       1700       1710       1720       1730       1740 
AKKPYQSQKL QKTKSSNKSQ IDPKYINLIK NKSRKHECQN MNKWISSTDK CTDDWEPIVE 

« Hide

References

« Hide 'large scale' references
[1]"A new example of viral intein in Mimivirus."
Ogata H., Raoult D., Claverie J.-M.
Virol. J. 2:8-8(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISCUSSION OF SEQUENCE.
[2]"The 1.2-megabase genome sequence of Mimivirus."
Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M.
Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rowbotham-Bradford.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY606804 Genomic DNA. Translation: AAU11330.1.
AY653733 Genomic DNA. Translation: AAV50591.1.
RefSeqYP_003986825.1. NC_014649.1.

3D structure databases

ProteinModelPortalQ5UQR0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9924939.

Family and domain databases

Gene3D3.10.28.10. 1 hit.
3.30.420.10. 3 hits.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 2 hits.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
SMARTSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 3 hits.
SSF55608. SSF55608. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDPOL_MIMIV
AccessionPrimary (citable) accession number: Q5UQR0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries