Reviewed, UniProtKB/Swiss-Prot Q5UQR0 (DPOL_MIMIV)

Names and origin

Protein names

Recommended name:
    DNA polymerase
    EC=2.7.7.7
Cleaved into the following chain:
    1- Recommended name:
            Mimv polB intein

Gene names

Name:	POLB
Ordered Locus Names:	MIMI_R322

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Complete proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	1740 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the DNA polymerase type-B family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	DNA replication
Domain	Repeat
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1052	1052	DNA polymerase, 1st part Potential		PRO_0000041777
	Chain	1053 – 1403	351	Mimv polB intein Potential		PRO_0000041778
	Chain	1404 – 1740	337	DNA polymerase, 2nd part Potential		PRO_0000041779
Regions
	Domain	1189 – 1334	146	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q5UQR0-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: DC8B2AFF4EEE7724	FASTA	1,740	201,945
	10 20 30 40 50 60 MPSETIDSTK QFEFQISDWN SYHELDQEEE EKYVIQLFGR TEDDHDVCLK VTGYTPFFYV 70 80 90 100 110 120 EIPKQWKQRQ VDKFVEILKN KVQYHCKKNL DEDFDLSKSL IKYAMVKKHK FYNFRNKQLY 130 140 150 160 170 180 NFLLLVFKSH TAMKEFSSIL ARPLEAKGLT NKPMLYQRYE SNIEPHIRFM HINNLSSCGW 190 200 210 220 230 240 ASIDKDKLKK IPEYSNCDYS FSVNWKDVKP SNNDDRMAPF KIMGYDIECV SCDQNFPQAE 250 260 270 280 290 300 RPSDKIIQIG ITMYRYGSMK CYEQHILTLK KCAPIEGVNV ECYKKEKGLL RGFAKKIAEL 310 320 330 340 350 360 RPDFKTGYNN FGFDDKYIYD RILRIDKREG KKQGVNINAL KNKFMDEILR TIGKVNNNYL 370 380 390 400 410 420 IENEGLDRIP IYTTVKDKKI SSKASRFIQI RGGTYVENGN NLKYVQSPGI TYFEVKNLSS 430 440 450 460 470 480 SALGDNELKF IQIPGVLSID MMKVIQRDHR LIGYKLDNVS ANFITEKADK IIEMPHNQED 490 500 510 520 530 540 SDSEKEDEDT DDKTYDVNIY TKSTKALEKD SYIQIMVNDG YSSSPLSEGA KYKVYDIQTI 550 560 570 580 590 600 TEKKLNEKTN KEEIFVYQAI KTKICQKDIQ QLRETIKNPL LGISWTFAKD DMHHTKINEY 610 620 630 640 650 660 FEEGDPKKIR QIAKYCLKDC KLVNLLLAKL EIIVNSVGMA KVCHVPLSYL FLRGQGVKIF 670 680 690 700 710 720 SLVSKKCREK NFLIPVLRRK SKDNEGDEDE TYEGATVITP KPNVYLSPIG VLDYSSLYPN 730 740 750 760 770 780 SMRERNLSQE CYVDDSKYDN LPGYIYHDVE IILKDKKGKI LRNIDGTPQK EYHRFAQEII 790 800 810 820 830 840 TDEQINRELK DIFDKINTVF ENNVAIIQNQ KYFTEKNISE LIDKHKNISD SKIEDIEFDE 850 860 870 880 890 900 SLSDKRKNKL VDAEKDSLDK NIGFYQKIKS QIDKIKLDSK IEIDNLSKNL NEEEKSKQIN 910 920 930 940 950 960 KMELNTKNLI SKVFSKYLIT EQQREELIVL EKERAKRSVN AEKAKVYNTV DGITVRYGIL 970 980 990 1000 1010 1020 PEILTELLNK RKETNGKLAN EKDPFVKAIL NALQLAFKVT ANSLYGQTGA PTSPLYFIAI 1030 1040 1050 1060 1070 1080 AACTTAIGRE RLHYAKKTVE DNFPGSEVIY GDSVTGDTPI ITRHQNGDIN ITTIEELGSK 1090 1100 1110 1120 1130 1140 WKPYEIFKAH EKNSNRKFKQ QSQYPTDSEV WTAKGWAKIK RVIRHKTVKK IYRVLTHTGC 1150 1160 1170 1180 1190 1200 IDVTEDHSLL DPNQNIIKPI NCQIGTELLH GFPESNNVYD NISEQEAYVW GFFMGDGSCG 1210 1220 1230 1240 1250 1260 SYQTKNGIKY SWALNNQDLD VLNKCKKYLE ETENIQFKIL DTMKSSSVYK LVPIRKIKYM 1270 1280 1290 1300 1310 1320 VNKYRKIFYD NKKYKLVPKE ILNSTKDIKN SFLEGYYAAD GSRKETENMG CRRCDIKGKI 1330 1340 1350 1360 1370 1380 SAQCLFYLLK SLGYNVSINI RSDKNQIYRL TFSNKKQRKN PIAIKKIQLM NETSNDHDGD 1390 1400 1410 1420 1430 1440 YVYDLETESG SFHAGVGEMI VKNTDSIFIN FHIKDENGEE KTDKEALMKT IAKCQRAAKL 1450 1460 1470 1480 1490 1500 INQNVPKPQS IVYEKTLHPF ILVAKKKYVG LLFEKSPDKY FLKSMGIVLK RRDNAPIVKI 1510 1520 1530 1540 1550 1560 VVGGIIDNIL KNRDIDKAIE YTKIVLDKLM NGEYPMDKFI ISKTLKSRYK KPSTIAHKVL 1570 1580 1590 1600 1610 1620 ADRMAVRDPG NKPQINDRIP FVYIVKDMGK KKKKDILQGD LIEHPEYVIA NNLKIDYLYY 1630 1640 1650 1660 1670 1680 LEHQIINPAS QILELMMDTK DVQKFFNKYI IDEQNKRKGA QSLTKWMDFS KLPKESGSKT 1690 1700 1710 1720 1730 1740 AKKPYQSQKL QKTKSSNKSQ IDPKYINLIK NKSRKHECQN MNKWISSTDK CTDDWEPIVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new example of viral intein in Mimivirus." Ogata H., Raoult D., Claverie J.-M. Virol. J. 2:8-8(2005) [PubMed: 15707490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISCUSSION OF SEQUENCE.
[2]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY606804 Genomic DNA. Translation: AAU11330.1. AY653733 Genomic DNA. Translation: AAV50591.1.
RefSeq	YP_142676.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3162567.
Enzyme and pathway databases
BRENDA	2.7.7.7. 281366.
Family and domain databases
InterPro	IPR006172. DNA-dir_DNA_pol_B. IPR006134. DNA-dir_DNA_pol_B_cons-reg. IPR006133. DNA-dir_DNA_pol_B_exonuc. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR012337. PolynucTfrase_RNaseH_fold. [Graphical view]
PANTHER	PTHR10322. DNA_pol_B. 1 hit.
Pfam	PF00136. DNA_pol_B. 1 hit. PF03104. DNA_pol_B_exo. 1 hit. [Graphical view]
SMART	SM00486. POLBc. 1 hit. [Graphical view]
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPOL_MIMIV

Accession

Primary (citable) accession number: Q5UQR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 7, 2004
Last modified:	November 24, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)