Reviewed, UniProtKB/Swiss-Prot Q5UQI4 (DHCR7_MIMIV)

Names and origin

Protein names

Recommended name:
    Probable 7-dehydrocholesterol reductase
      Short name=7-DHC reductase
    EC=1.3.1.21
Alternative name(s):
    Sterol Delta(7)-reductase

Gene names

Name:	DHCR7
Ordered Locus Names:	MIMI_R807

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Complete proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC) By similarity.
Catalytic activity	Cholesterol + NADP+ = cholesta-5,7-dien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; cholesterol biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	7-dehydrocholesterol reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Probable 7-dehydrocholesterol reductase		PRO_0000207508
Regions
	Transmembrane	24 – 44	21	Potential
	Transmembrane	71 – 91	21	Potential
	Transmembrane	102 – 124	23	Potential
	Transmembrane	133 – 153	21	Potential
	Transmembrane	157 – 177	21	Potential
	Transmembrane	244 – 264	21	Potential
	Transmembrane	281 – 301	21	Potential
	Transmembrane	309 – 329	21	Potential
	Transmembrane	393 – 413	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5UQI4-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: F338970A8F7AA48D	FASTA	447	51,692
	10 20 30 40 50 60 MNSYQTNTAT SWGRNHIPTL LDNLTTAAMF MFCPFIILVF YLITYGEYLG SIGDFYLDII 70 80 90 100 110 120 NGDWQTIWSN IPSFKINVLG ACLLWIVFQL ILSKLPDTIH RFVPHYVGGI KAGHITPAGN 130 140 150 160 170 180 LVYYNINGLQ AFIITHVLVI MSCYYGLFSP TIIMDNWGSI FWSVNIIGYL ITFLAYFKAL 190 200 210 220 230 240 TFSSHPSDNK FTGKLFYDIV MGIEFNPEIF GTDLKLFFNG RPGIIAWNLI NLSCAMKQYE 250 260 270 280 290 300 NFGYVSNSMI LVIILQLIYI VDFFYNENWY VHTVDIAHDH FGWMLAWGDT VWLPFGYTLQ 310 320 330 340 350 360 AGYLMNNPID LSTGFFNLVF VMGIIGYIIF RTANYQKDKY RSNTQGVKYI PCTYQTADGL 370 380 390 400 410 420 NRASKLIYSG LWGVSRHMNY TGDIILSTAY CLACGFSHFI PYFYCVYMTI LLVTRCLRDE 430 440 QRCSRKYGKY WKMYTKRVPY RFIPGIY

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY653733 Genomic DNA. Translation: AAV51067.1.
RefSeq	YP_143161.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3162460.
Enzyme and pathway databases
BRENDA	1.3.1.21. 281366.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHCR7_MIMIV

Accession

Primary (citable) accession number: Q5UQI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 7, 2004
Last modified:	October 13, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)