Reviewed, UniProtKB/Swiss-Prot Q5UQI3 (CP51_MIMIV)

Names and origin

Protein names

Recommended name:
    Probable lanosterol 14-alpha demethylase
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    CYPLI
    P450-LIA1
    Sterol 14-alpha demethylase
    P450-14DM

Gene names

Ordered Locus Names:

MIMI_L808

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Complete proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Methyltransferase Monooxygenase Oxidoreductase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 709	709	Probable lanosterol 14-alpha demethylase		PRO_0000052015
Sites
	Metal binding	425	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UQI3-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: FCE6AF553DEF2F6F	FASTA	709	82,186
	10 20 30 40 50 60 MLFELSIGAI IGFLTLYLLK RFNESKNFIT PDNLKKIPIV EGAVPVLGHG PAFSKDIMQF 70 80 90 100 110 120 MKNCYKKYGS VFQLKIFRTN MVVLCDRKLS EEFFKSREND MSLYDVLNRL FFGLAFSDKP 130 140 150 160 170 180 DSLEFIIKMV KKTITIRYDD FAPKIMDEAQ RLTKIMRESH SGKKLDMIPE IIKFVSRTSA 190 200 210 220 230 240 RCFIAMDIDD EFYDALNKFT NLLNKIVVLT YFVPHWLLNA TLNRFMLRKY RMRMTKLLEN 250 260 270 280 290 300 EIEKYRTDLN KSDSLLFRKC VDHIDPETGA TLTNQDIGDI VVCLLYVSSE NTSLLATNCL 310 320 330 340 350 360 IDLTLNPKYW DLIKSECSAM IALGDYKNLF KAPLLNSIVM ESARLNSHVF ALARKPKTVN 370 380 390 400 410 420 RIGDYFVADN VDTISLCEPA LMKFEIASDV YANPNSYDPV RFMAPRNEPK DSGHVMNWGK 430 440 450 460 470 480 GVHECPGKQF AIYEVKAAIA YIVTNFERFE FNHNDLKINY FSPSAMCEKN ISVEFIPSQQ 490 500 510 520 530 540 NIHNIVYKDR TYIVEHIKCN ETSAWLIYNA LDRQQQREYY QYTYEISTDS QEHKLIEKAG 550 560 570 580 590 600 PHKPFPIAYD KLVYTGQSNC MTPTKWYDFA SDIWELLTEN YAELGFPIYD DKIRNFVPNS 610 620 630 640 650 660 FYGQLYSVES IMPTHRDQHV DYGLSISIGS NCEFVIEDKT ILLPSGSVLI GDFSKISHSV 670 680 690 700 SKIFHEKPDH LSDFEFFNRV RFSAQIRSID PDVQPLMTTQ EFLDMISEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.
[2]	"The first virally encoded cytochrome p450." Lamb D.C., Lei L., Warrilow A.G., Lepesheva G.I., Mullins J.G., Waterman M.R., Kelly S.L. J. Virol. 83:8266-8269(2009) [PubMed: 19515774] [Abstract] Cited for: FUNCTION. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY653733 Genomic DNA. Translation: AAV51068.1.
RefSeq	YP_143162.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3162320.
Enzyme and pathway databases
BRENDA	1.14.13.70. 281366.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_MIMIV

Accession

Primary (citable) accession number: Q5UQI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 7, 2004
Last modified:	September 22, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)