Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5UQG3

- DRTS_MIMIV

UniProt

Q5UQG3 - DRTS_MIMIV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

MIMI_R497

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei292 – 2921dUMPBy similarity
Active sitei435 – 4351By similarity
Binding sitei436 – 4361dUMPBy similarity
Binding sitei474 – 4741dUMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi117 – 1248NADPBy similarity
Nucleotide bindingi464 – 4685dUMPBy similarity
Nucleotide bindingi504 – 5063dUMPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. glycine biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis, One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
Gene namesi
Ordered Locus Names:MIMI_R497
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 563563Bifunctional dihydrofolate reductase-thymidylate synthasePRO_0000186360Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5UQG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 195193DHFRAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni275 – 563289Thymidylate synthaseBy similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
In the C-terminal section; belongs to the thymidylate synthase family.Curated

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5UQG3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKFNIIAAI NNDSIIGVKE YGTFSMPWPY LKDDMNHFRK ITTDTGSIES
60 70 80 90 100
GVNAIIVGFN TWQTLPSSYR NIRSRFNIVI SRDDETDGQF HKYVKTFDEA
110 120 130 140 150
IEFASSLTNL NEIFVIGGGV IYDLALKHKL LDKLYLTHVG SNYPIDDNVE
160 170 180 190 200
KVVHFPLTWS KIEKMCDSNF LELDSEISKH DIGKNILLRF QEYSVKKELY
210 220 230 240 250
WAIEYLKKNT SLDNKGIIGD KGATGSKGYS LEQHDYYSTE YFWNFYEFIT
260 270 280 290 300
RKVFDLFNSS NEISIPSEEC PENQYIELVK TIMEKGIVKQ TRNSITKSIF
310 320 330 340 350
GYQLKYDLSK GYPIQTIKRS YPKAIFEELM WMIRGQTDVS ILQKKGVHVW
360 370 380 390 400
DKNSSKDFLS KYNLPYEEGD IGPGYGFQMR YWGAEYTDCK TSYQGQGIDQ
410 420 430 440 450
LNKCIESIQN NPHDRRIMIN LWNCSDLDKM ALAPCHFCYM FGVDLYEVPT
460 470 480 490 500
TSGKKGRLNC HLVQRSWDVL LGWNTTTAAL LTYLIANHCD LDPGILVHSI
510 520 530 540 550
SDAHIYQSHI DSGAISQLLQ RKCRKFPNLV IRNKKEKIDD YEFDDLIIEN
560
YYPCPSISAE MIA
Length:563
Mass (Da):65,063
Last modified:December 7, 2004 - v1
Checksum:i590F4EAE5A11625B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAV50762.1.
RefSeqiYP_003987009.1. NC_014649.1.

Genome annotation databases

GeneIDi9925127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAV50762.1 .
RefSeqi YP_003987009.1. NC_014649.1.

3D structure databases

ProteinModelPortali Q5UQG3.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9925127.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR024072. DHFR-like_dom.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 1 hit.
PROSITEi PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.

Entry informationi

Entry nameiDRTS_MIMIV
AccessioniPrimary (citable) accession number: Q5UQG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: October 1, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3