Reviewed, UniProtKB/Swiss-Prot Q5UQG3 (DRTS_MIMIV)

Names and origin

Protein names

Recommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthase
      Short name=DHFR-TS
Including the following 2 domains:
    1- Recommended name:
            Dihydrofolate reductase
              EC=1.5.1.3
    2- Recommended name:
            Thymidylate synthase
              EC=2.1.1.45

Gene names

Ordered Locus Names:

MIMI_R497

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Complete proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH. 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	In the N-terminal section; belongs to the dihydrofolate reductase family. In the C-terminal section; belongs to the thymidylate synthase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis One-carbon metabolism
Ligand	NADP
Molecular function	Methyltransferase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro glycine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro thymidylate synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	Bifunctional dihydrofolate reductase-thymidylate synthase		PRO_0000186360
Regions
	Domain	3 – 195	193	DHFR
	Region	275 – 563	289	Thymidylate synthase By similarity
Sites
	Active site	435	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UQG3-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 590F4EAE5A11625B	FASTA	563	65,063
	10 20 30 40 50 60 MKKFNIIAAI NNDSIIGVKE YGTFSMPWPY LKDDMNHFRK ITTDTGSIES GVNAIIVGFN 70 80 90 100 110 120 TWQTLPSSYR NIRSRFNIVI SRDDETDGQF HKYVKTFDEA IEFASSLTNL NEIFVIGGGV 130 140 150 160 170 180 IYDLALKHKL LDKLYLTHVG SNYPIDDNVE KVVHFPLTWS KIEKMCDSNF LELDSEISKH 190 200 210 220 230 240 DIGKNILLRF QEYSVKKELY WAIEYLKKNT SLDNKGIIGD KGATGSKGYS LEQHDYYSTE 250 260 270 280 290 300 YFWNFYEFIT RKVFDLFNSS NEISIPSEEC PENQYIELVK TIMEKGIVKQ TRNSITKSIF 310 320 330 340 350 360 GYQLKYDLSK GYPIQTIKRS YPKAIFEELM WMIRGQTDVS ILQKKGVHVW DKNSSKDFLS 370 380 390 400 410 420 KYNLPYEEGD IGPGYGFQMR YWGAEYTDCK TSYQGQGIDQ LNKCIESIQN NPHDRRIMIN 430 440 450 460 470 480 LWNCSDLDKM ALAPCHFCYM FGVDLYEVPT TSGKKGRLNC HLVQRSWDVL LGWNTTTAAL 490 500 510 520 530 540 LTYLIANHCD LDPGILVHSI SDAHIYQSHI DSGAISQLLQ RKCRKFPNLV IRNKKEKIDD 550 560 YEFDDLIIEN YYPCPSISAE MIA

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY653733 Genomic DNA. Translation: AAV50762.1.
RefSeq	YP_142851.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3162308.
Family and domain databases
InterPro	IPR001796. DHFR_reg. IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR. PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00075. DHFR_1. False negative. PS51330. DHFR_2. 1 hit. PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DRTS_MIMIV

Accession

Primary (citable) accession number: Q5UQG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 7, 2004
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)