SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5UQE1

- ASNS_MIMIV

UniProt

Q5UQE1 - ASNS_MIMIV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Probable asparagine synthetase [glutamine-hydrolyzing]
Gene
MIMI_R475
Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Binding sitei100 – 1001Glutamine By similarity
Binding sitei256 – 2561ATP; via carbonyl oxygen By similarity
Binding sitei284 – 2841ATP; via amide nitrogen and carbonyl oxygen By similarity
Sitei362 – 3621Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi360 – 3612ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC

GO - Biological processi

  1. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Ordered Locus Names:MIMI_R475
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host By similarity
Chaini2 – 550549Probable asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056930Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5UQE1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 189188Glutamine amidotransferase type-2
Add
BLAST
Domaini213 – 530318Asparagine synthetase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 575Glutamine binding By similarity
Regioni78 – 803Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5UQE1-1 [UniParc]FASTAAdd to Basket

« Hide

MCGIICFIQY GGQKIDLVSC LNCLDKLNNR GPDAQSYQVI ELGDITIFLG    50
FTRLAIMDTS EAGLQPFKDN NSNYSICNGE IYNYKNLAEK FNIEMQSQCD 100
CEILLPLFNL RGFEGLLSDL DAEFATVIVD KYNSKLYAAR DKYGVRPLYY 150
GYNCEKGLIG FASELKALHS VMEYVEQVKP NQYVTIDLSF RPSIPFDLQN 200
FVKLFQFTNY HEYYQSHKSL IDYHEPNIEQ LQTSINHLLT EAVRKRLYAD 250
RQIGFLLSGG LDSSLIVAIA TRLLGPTNIV CFSVGFEGSP DVAAAREVVK 300
FLGIKNHHIV PFSVDIGLNA INDVIKTIET YDITTIRAST PQFIMAKYIQ 350
ENTDIRVLLS GEGSDEIHGS YKYMRSAPNS QEFHKETIRL LEELYLFDNK 400
RTDRTMAGNG LEVRVPFLDF NYVDFIMNID PNLLMYKSDY IEKKIIRDAF 450
KGYLPENILY RPKEAFSDAV SSKEINWYRS IQKITEEIYT DEKLQNSNYK 500
FNKPEIKEAL YYRDIFNSHY GGRDHIIPHY WLPRFQQNNV LDPSATILPI 550
Length:550
Mass (Da):63,335
Last modified:January 23, 2007 - v3
Checksum:i68800D6D26B47778
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAV50741.1.
RefSeqiYP_003986982.1. NC_014649.1.

Genome annotation databases

GeneIDi9925100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAV50741.1 .
RefSeqi YP_003986982.1. NC_014649.1.

3D structure databases

ProteinModelPortali Q5UQE1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9925100.

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.

Entry informationi

Entry nameiASNS_MIMIV
AccessioniPrimary (citable) accession number: Q5UQE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 55 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi