Reviewed, UniProtKB/Swiss-Prot Q5UQE1 (ASNS_MIMIV)

Names and origin

Protein names

Recommended name:
    Probable asparagine synthetase [glutamine-hydrolyzing]
    EC=6.3.5.4
Alternative name(s):
    Glutamine-dependent asparagine synthetase

Gene names

Ordered Locus Names:

MIMI_R475

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Complete proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
Sequence similarities	Contains 1 asparagine synthetase domain. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; by host By similarity
	Chain	2 – 550	549	Probable asparagine synthetase [glutamine-hydrolyzing]		PRO_0000056930
Regions
	Domain	2 – 189	188	Glutamine amidotransferase type-2
	Domain	213 – 530	318	Asparagine synthetase
Sites
	Active site	2	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UQE1-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 68800D6D26B47778	FASTA	550	63,335
	10 20 30 40 50 60 MCGIICFIQY GGQKIDLVSC LNCLDKLNNR GPDAQSYQVI ELGDITIFLG FTRLAIMDTS 70 80 90 100 110 120 EAGLQPFKDN NSNYSICNGE IYNYKNLAEK FNIEMQSQCD CEILLPLFNL RGFEGLLSDL 130 140 150 160 170 180 DAEFATVIVD KYNSKLYAAR DKYGVRPLYY GYNCEKGLIG FASELKALHS VMEYVEQVKP 190 200 210 220 230 240 NQYVTIDLSF RPSIPFDLQN FVKLFQFTNY HEYYQSHKSL IDYHEPNIEQ LQTSINHLLT 250 260 270 280 290 300 EAVRKRLYAD RQIGFLLSGG LDSSLIVAIA TRLLGPTNIV CFSVGFEGSP DVAAAREVVK 310 320 330 340 350 360 FLGIKNHHIV PFSVDIGLNA INDVIKTIET YDITTIRAST PQFIMAKYIQ ENTDIRVLLS 370 380 390 400 410 420 GEGSDEIHGS YKYMRSAPNS QEFHKETIRL LEELYLFDNK RTDRTMAGNG LEVRVPFLDF 430 440 450 460 470 480 NYVDFIMNID PNLLMYKSDY IEKKIIRDAF KGYLPENILY RPKEAFSDAV SSKEINWYRS 490 500 510 520 530 540 IQKITEEIYT DEKLQNSNYK FNKPEIKEAL YYRDIFNSHY GGRDHIIPHY WLPRFQQNNV 550 LDPSATILPI

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY653733 Genomic DNA. Translation: AAV50741.1.
RefSeq	YP_142829.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3162085.
Enzyme and pathway databases
BRENDA	6.3.5.4. 281366.
Family and domain databases
InterPro	IPR006426. Asn_synth_AEB. IPR001962. Asn_synthase. IPR000583. GATase_2. IPR017932. GATase_II. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00733. Asn_synthase. 1 hit. PF00310. GATase_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01536. asn_synth_AEB. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNS_MIMIV

Accession

Primary (citable) accession number: Q5UQE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 33 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)