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Q5UQE1 (ASNS_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable asparagine synthetase [glutamine-hydrolyzing]

EC=6.3.5.4
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene names
Ordered Locus Names:MIMI_R475
OrganismAcanthamoeba polyphaga mimivirus (APMV) [Reference proteome]
Taxonomic identifier212035 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length550 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Sequence similarities

Contains 1 asparagine synthetase domain.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host By similarity
Chain2 – 550549Probable asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056930

Regions

Domain2 – 189188Glutamine amidotransferase type-2
Domain213 – 530318Asparagine synthetase
Nucleotide binding360 – 3612ATP By similarity
Region53 – 575Glutamine binding By similarity
Region78 – 803Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site1001Glutamine By similarity
Binding site2561ATP; via carbonyl oxygen By similarity
Binding site2841ATP; via amide nitrogen and carbonyl oxygen By similarity
Site3621Important for beta-aspartyl-AMP intermediate formation By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5UQE1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 68800D6D26B47778

FASTA55063,335
        10         20         30         40         50         60 
MCGIICFIQY GGQKIDLVSC LNCLDKLNNR GPDAQSYQVI ELGDITIFLG FTRLAIMDTS 

        70         80         90        100        110        120 
EAGLQPFKDN NSNYSICNGE IYNYKNLAEK FNIEMQSQCD CEILLPLFNL RGFEGLLSDL 

       130        140        150        160        170        180 
DAEFATVIVD KYNSKLYAAR DKYGVRPLYY GYNCEKGLIG FASELKALHS VMEYVEQVKP 

       190        200        210        220        230        240 
NQYVTIDLSF RPSIPFDLQN FVKLFQFTNY HEYYQSHKSL IDYHEPNIEQ LQTSINHLLT 

       250        260        270        280        290        300 
EAVRKRLYAD RQIGFLLSGG LDSSLIVAIA TRLLGPTNIV CFSVGFEGSP DVAAAREVVK 

       310        320        330        340        350        360 
FLGIKNHHIV PFSVDIGLNA INDVIKTIET YDITTIRAST PQFIMAKYIQ ENTDIRVLLS 

       370        380        390        400        410        420 
GEGSDEIHGS YKYMRSAPNS QEFHKETIRL LEELYLFDNK RTDRTMAGNG LEVRVPFLDF 

       430        440        450        460        470        480 
NYVDFIMNID PNLLMYKSDY IEKKIIRDAF KGYLPENILY RPKEAFSDAV SSKEINWYRS 

       490        500        510        520        530        540 
IQKITEEIYT DEKLQNSNYK FNKPEIKEAL YYRDIFNSHY GGRDHIIPHY WLPRFQQNNV 

       550 
LDPSATILPI 

« Hide

References

[1]"The 1.2-megabase genome sequence of Mimivirus."
Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M.
Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rowbotham-Bradford.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY653733 Genomic DNA. Translation: AAV50741.1.
RefSeqYP_003986982.1. NC_014649.1.

3D structure databases

ProteinModelPortalQ5UQE1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9925100.

Phylogenomic databases

ProtClustDBCLSP2512637.

Enzyme and pathway databases

UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNS_MIMIV
AccessionPrimary (citable) accession number: Q5UQE1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 53 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways