ID   PNKP_MIMIV              Reviewed;         421 AA.
AC   Q5UQD2;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   20-JAN-2009, entry version 24.
DE   RecName: Full=Putative bifunctional polynucleotide phosphatase/kinase;
DE   AltName: Full=Polynucleotide kinase-3'-phosphatase;
DE   AltName: Full=DNA 5'-kinase/3'-phosphatase;
DE   Includes:
DE     RecName: Full=Polynucleotide 3'-phosphatase;
DE              EC=3.1.3.32;
DE     AltName: Full=2'(3')-polynucleotidase;
DE   Includes:
DE     RecName: Full=Polynucleotide 5'-hydroxyl-kinase;
DE              EC=2.7.1.78;
GN   OrderedLocusNames=MIMI_L469;
OS   Acanthamoeba polyphaga mimivirus (APMV).
OC   Viruses; dsDNA viruses, no RNA stage; Mimiviridae; Mimivirus.
OX   NCBI_TaxID=212035;
OH   NCBI_TaxID=5757; Acanthamoeba polyphaga (Amoeba).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Rowbotham-Bradford;
RX   PubMed=15486256; DOI=10.1126/science.1101485;
RA   Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H.,
RA   La Scola B., Susan M., Claverie J.-M.;
RT   "The 1.2-megabase genome sequence of Mimivirus.";
RL   Science 306:1344-1350(2004).
CC   -!- CATALYTIC ACTIVITY: A 3'-phosphopolynucleotide + H(2)O = a
CC       polynucleotide + phosphate.
CC   -!- CATALYTIC ACTIVITY: ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the DNA 3'
CC       phosphatase family.
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DR   EMBL; AY653733; AAV50735.1; -; Genomic_DNA.
DR   RefSeq; YP_142823.1; -.
DR   GeneID; 3162093; -.
DR   BRENDA; 2.7.1.78; 281366.
DR   BRENDA; 3.1.3.32; 281366.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046404; F:ATP-dependent polydeoxyribonucleotide 5'-hy...; IEA:EC.
DR   GO; GO:0046403; F:polynucleotide 3'-phosphatase activity; IEA:EC.
DR   InterPro; IPR006551; DNA-3-Pase.
DR   InterPro; IPR013954; PNK3P_central-region.
DR   InterPro; IPR015636; PNK_3Pase.
DR   PANTHER; PTHR12083; PNK_3Pase; 1.
DR   Pfam; PF08645; PNK3P; 1.
DR   TIGRFAMs; TIGR01664; DNA-3_-Pase; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Hydrolase; Kinase;
KW   Multifunctional enzyme; Nucleotide-binding; Transferase.
FT   CHAIN         1    421       Putative bifunctional polynucleotide
FT                                phosphatase/kinase.
FT                                /FTId=PRO_0000247288.
FT   NP_BIND     265    272       ATP (Potential).
SQ   SEQUENCE   421 AA;  49258 MW;  CB089165B865F006 CRC64;
     MRYYRITIIR FINKYTKIMD WIESDSYLKG IINNKPKLHD PVRVASFDLD DTLIVRSKKT
     QKWKLVDSSI KQKIAELIEN KYIIIVFTNQ GGMSLNKKFD KPLWRKAMDD LVKILTSETD
     NDFYFAIYVA KKYDIYRKPN IGLWNLMKQD IKDEFNLDSV QISTKSFFCG DAAGRIYPSM
     FKKKLYPTSK GGDFSDTDRK FALNIGIKFL TPEEFYLDSK NSENLKTNYK LSGVNPTEII
     DEIENTKLVN YKFKPRKKEM IVMIGQPGSG KSFFVKNYIL PNGYVHINQD KCKTKAKCLS
     ETENALSKGK SVVIDNTNPD VISRMTYTNL AKENNYDHVR AIIMETPDEL AKHLNNVRHI
     YSSGTVPKVT DIAYNIYRKN FVLPQYEENF DKIETVTFYF DKSMLDDPKW KRSFMKFSEY
     K
//