Reviewed, UniProtKB/Swiss-Prot Q5UQD2 (PNKP_MIMIV)

Names and origin

Protein names

Recommended name:
    Putative bifunctional polynucleotide phosphatase/kinase
Alternative name(s):
    Polynucleotide kinase-3'-phosphatase
    DNA 5'-kinase/3'-phosphatase
Including the following 2 domains:
    1- Recommended name:
            Polynucleotide 3'-phosphatase
              EC=3.1.3.32
        Alternative name(s):
            2'(3')-polynucleotidase
    2- Recommended name:
            Polynucleotide 5'-hydroxyl-kinase
              EC=2.7.1.78

Gene names

Ordered Locus Names:

MIMI_L469

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Complete proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate. ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.
Sequence similarities	In the N-terminal section; belongs to the DNA 3' phosphatase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Inferred from electronic annotation. Source: EC polynucleotide 3'-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Putative bifunctional polynucleotide phosphatase/kinase		PRO_0000247288
Regions
	Nucleotide binding	265 – 272	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5UQD2-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: CB089165B865F006	FASTA	421	49,258
	10 20 30 40 50 60 MRYYRITIIR FINKYTKIMD WIESDSYLKG IINNKPKLHD PVRVASFDLD DTLIVRSKKT 70 80 90 100 110 120 QKWKLVDSSI KQKIAELIEN KYIIIVFTNQ GGMSLNKKFD KPLWRKAMDD LVKILTSETD 130 140 150 160 170 180 NDFYFAIYVA KKYDIYRKPN IGLWNLMKQD IKDEFNLDSV QISTKSFFCG DAAGRIYPSM 190 200 210 220 230 240 FKKKLYPTSK GGDFSDTDRK FALNIGIKFL TPEEFYLDSK NSENLKTNYK LSGVNPTEII 250 260 270 280 290 300 DEIENTKLVN YKFKPRKKEM IVMIGQPGSG KSFFVKNYIL PNGYVHINQD KCKTKAKCLS 310 320 330 340 350 360 ETENALSKGK SVVIDNTNPD VISRMTYTNL AKENNYDHVR AIIMETPDEL AKHLNNVRHI 370 380 390 400 410 420 YSSGTVPKVT DIAYNIYRKN FVLPQYEENF DKIETVTFYF DKSMLDDPKW KRSFMKFSEY K

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY653733 Genomic DNA. Translation: AAV50735.1.
RefSeq	YP_142823.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3162093.
Enzyme and pathway databases
BRENDA	2.7.1.78. 281366. 3.1.3.32. 281366.
Family and domain databases
InterPro	IPR006551. DNA-3-Pase. IPR013954. PNK3P_central-region. IPR015636. PNK_3Pase. [Graphical view]
PANTHER	PTHR12083. PNK_3Pase. 1 hit.
Pfam	PF08645. PNK3P. 1 hit. [Graphical view]
TIGRFAMs	TIGR01664. DNA-3_-Pase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNKP_MIMIV

Accession

Primary (citable) accession number: Q5UQD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	December 7, 2004
Last modified:	January 20, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)