Q5UQD2 (PNKP_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative bifunctional polynucleotide phosphatase/kinase

Alternative name(s):
DNA 5'-kinase/3'-phosphatase
Polynucleotide kinase-3'-phosphatase

Including the following 2 domains:

1. Polynucleotide 3'-phosphatase
   EC=3.1.3.32
   Alternative name(s):
   2'(3')-polynucleotidase
2. Polynucleotide 5'-hydroxyl-kinase
   EC=2.7.1.78

Gene names

Ordered Locus Names:

MIMI_L469

Organism

Acanthamoeba polyphaga mimivirus (APMV)

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate. ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.
Sequence similarities	In the N-terminal section; belongs to the DNA 3' phosphatase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Inferred from electronic annotation. Source: EC polynucleotide 3'-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Putative bifunctional polynucleotide phosphatase/kinase		PRO_0000247288
Regions
	Nucleotide binding	265 – 272	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5UQD2 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: CB089165B865F006	FASTA	421	49,258
	10 20 30 40 50 60 MRYYRITIIR FINKYTKIMD WIESDSYLKG IINNKPKLHD PVRVASFDLD DTLIVRSKKT 70 80 90 100 110 120 QKWKLVDSSI KQKIAELIEN KYIIIVFTNQ GGMSLNKKFD KPLWRKAMDD LVKILTSETD 130 140 150 160 170 180 NDFYFAIYVA KKYDIYRKPN IGLWNLMKQD IKDEFNLDSV QISTKSFFCG DAAGRIYPSM 190 200 210 220 230 240 FKKKLYPTSK GGDFSDTDRK FALNIGIKFL TPEEFYLDSK NSENLKTNYK LSGVNPTEII 250 260 270 280 290 300 DEIENTKLVN YKFKPRKKEM IVMIGQPGSG KSFFVKNYIL PNGYVHINQD KCKTKAKCLS 310 320 330 340 350 360 ETENALSKGK SVVIDNTNPD VISRMTYTNL AKENNYDHVR AIIMETPDEL AKHLNNVRHI 370 380 390 400 410 420 YSSGTVPKVT DIAYNIYRKN FVLPQYEENF DKIETVTFYF DKSMLDDPKW KRSFMKFSEY K

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY653733 Genomic DNA. Translation: AAV50735.1.
RefSeq	YP_003986976.1. NC_014649.1.
3D structure databases
ProteinModelPortal	Q5UQD2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9925093.
Family and domain databases
InterPro	IPR023214. HAD-like_dom. IPR006549. HAD-SF_hydro_IIIA. IPR013954. PNK3P. IPR015636. PNK_3Pase_euk. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
PANTHER	PTHR12083. PNK_3Pase. 1 hit.
Pfam	PF08645. PNK3P. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01662. HAD-SF-IIIA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNKP_MIMIV

Accession

Primary (citable) accession number: Q5UQD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	December 7, 2004
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program