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Protein

DNA topoisomerase 1 type prokaryotic

Gene

TOP1P

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactori

Mg2+By similarityNote: Binds two Mg2+ per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Magnesium 1; catalyticBy similarity1
Metal bindingi79Magnesium 1; catalyticBy similarity1
Metal bindingi79Magnesium 2By similarity1
Metal bindingi81Magnesium 2By similarity1
Active sitei302O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 type prokaryotic (EC:5.99.1.2)
Gene namesi
Name:TOP1P
Ordered Locus Names:MIMI_L221
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
Proteomesi
  • UP000001134 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • virion Source: UniProtKB-SubCell

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451831 – 854DNA topoisomerase 1 type prokaryoticAdd BLAST854

Proteomic databases

PRIDEiQ5UQB5

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Interaction with DNABy similarity1
Sitei134Interaction with DNABy similarity1
Sitei135Interaction with DNABy similarity1
Sitei138Interaction with DNABy similarity1
Sitei143Interaction with DNABy similarity1
Sitei304Interaction with DNABy similarity1
Sitei505Interaction with DNABy similarity1

Structurei

3D structure databases

ProteinModelPortaliQ5UQB5
SMRiQ5UQB5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 110ToprimAdd BLAST109

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni158 – 163Interaction with DNABy similarity6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated

Phylogenomic databases

OrthoDBiVOG0900001M

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 3 hits
2.70.20.10, 2 hits
HAMAPiMF_00952 Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005733 TopoI_bac-type
IPR028612 Topoisom_1_IA
IPR025589 Toprim_C_rpt
IPR006171 TOPRIM_domain
PANTHERiPTHR42785 PTHR42785, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 2 hits
PF01751 Toprim, 1 hit
PF13368 Toprim_C_rpt, 3 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 2 hits
TIGRFAMsiTIGR01051 topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q5UQB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILILLESP GKISKISSIL GKNYVVKASM GHFRDLDPKK MSIDFDNDFE
60 70 80 90 100
PVYIVTKPDV VKNLKSAMKN IDLVYLAADE DREGEAIAQS LYDVLKPSNY
110 120 130 140 150
KRLRFNAITK DAIMSAIKNA GDIDKNLVDA QKARRVLDRL FGYLISPILQ
160 170 180 190 200
RQIGGKLSAG RVQSVTVRII IDKENEIKNF INKNADSSYF KVSGTFNGAK
210 220 230 240 250
ATLHESNDKK PFDLETAYKG KTAQIALINS ENPNSKVVNF MKRCLKSQFF
260 270 280 290 300
IHSVEDKMTT RSPAPPFTTS TLQQEANRKF GMSIDSTMKT AQKLYEGGYI
310 320 330 340 350
TYMRTDSVEI SAEGHRDIKK IITDQYGADY YQKNLYKNKA ANSQEAHEAI
360 370 380 390 400
RPTHPELLTL EGEIEDAYQI KLYKLIWQRT IASQMKPAKI KVTIIQISIS
410 420 430 440 450
KYVEDKLNPF YYFQSQIETV VFPGFMKVYV ESIDDPDTDN QITKNFTGKI
460 470 480 490 500
PTVGSKVTME EIIARQEYMR PPPRYSEASL VKKLEELGIG RPSTYVNTIK
510 520 530 540 550
TIINREYVKI TDVPGIKKDI TIYSIKSENK KHIMEVYEDT DTILLGKENK
560 570 580 590 600
KIVPTNLGIT VNDFLMKYFP EFLDYKFTAN METDLDYVST GTKNWVDIVQ
610 620 630 640 650
DFYDKLKPIV DELSKQKGLS QSSERLLGED NDGNEITATK TKFGPVVRKK
660 670 680 690 700
IGDKYVYAKI KDPLTLDTIK LSDAIKLLEY PKNLGQYKGF DVLLQKGDYG
710 720 730 740 750
FYLSYNKENF SLGEIDDPED INLDTAIKAI EAKKANNIAE FNLTENGKKI
760 770 780 790 800
KAIVLNGKYG YYVQVTRNRI KKNYPIPKDL DPNNLTEQQI LSIISVKKTY
810 820 830 840 850
KKSAPKGGSK TIRKPSQTKY SQTKSTKSTK STKSTNKKFV GKSAKKTTKK

TTKK
Length:854
Mass (Da):96,946
Last modified:December 7, 2004 - v1
Checksum:i2D7361B66C8C0C0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA Translation: AAV50494.1
RefSeqiYP_003986717.1, NC_014649.1

Genome annotation databases

GeneIDi9924828
KEGGivg:9924828

Similar proteinsi

Entry informationi

Entry nameiTOP1P_MIMIV
AccessioniPrimary (citable) accession number: Q5UQB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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