Q5UQB5 (TOP1P_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1 type prokaryotic
EC=5.99.1.2

Gene names

Name:	TOP1P
Ordered Locus Names:	MIMI_L221

Organism

Acanthamoeba polyphaga mimivirus (APMV)

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	854 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subcellular location	Virion Ref.2.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Cellular component	Virion
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 854	854	DNA topoisomerase 1 type prokaryotic		PRO_0000145183
Regions
	Domain	2 – 106	105	Toprim
Sites
	Active site	302	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UQB5 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 2D7361B66C8C0C0C	FASTA	854	96,946
	10 20 30 40 50 60 MSILILLESP GKISKISSIL GKNYVVKASM GHFRDLDPKK MSIDFDNDFE PVYIVTKPDV 70 80 90 100 110 120 VKNLKSAMKN IDLVYLAADE DREGEAIAQS LYDVLKPSNY KRLRFNAITK DAIMSAIKNA 130 140 150 160 170 180 GDIDKNLVDA QKARRVLDRL FGYLISPILQ RQIGGKLSAG RVQSVTVRII IDKENEIKNF 190 200 210 220 230 240 INKNADSSYF KVSGTFNGAK ATLHESNDKK PFDLETAYKG KTAQIALINS ENPNSKVVNF 250 260 270 280 290 300 MKRCLKSQFF IHSVEDKMTT RSPAPPFTTS TLQQEANRKF GMSIDSTMKT AQKLYEGGYI 310 320 330 340 350 360 TYMRTDSVEI SAEGHRDIKK IITDQYGADY YQKNLYKNKA ANSQEAHEAI RPTHPELLTL 370 380 390 400 410 420 EGEIEDAYQI KLYKLIWQRT IASQMKPAKI KVTIIQISIS KYVEDKLNPF YYFQSQIETV 430 440 450 460 470 480 VFPGFMKVYV ESIDDPDTDN QITKNFTGKI PTVGSKVTME EIIARQEYMR PPPRYSEASL 490 500 510 520 530 540 VKKLEELGIG RPSTYVNTIK TIINREYVKI TDVPGIKKDI TIYSIKSENK KHIMEVYEDT 550 560 570 580 590 600 DTILLGKENK KIVPTNLGIT VNDFLMKYFP EFLDYKFTAN METDLDYVST GTKNWVDIVQ 610 620 630 640 650 660 DFYDKLKPIV DELSKQKGLS QSSERLLGED NDGNEITATK TKFGPVVRKK IGDKYVYAKI 670 680 690 700 710 720 KDPLTLDTIK LSDAIKLLEY PKNLGQYKGF DVLLQKGDYG FYLSYNKENF SLGEIDDPED 730 740 750 760 770 780 INLDTAIKAI EAKKANNIAE FNLTENGKKI KAIVLNGKYG YYVQVTRNRI KKNYPIPKDL 790 800 810 820 830 840 DPNNLTEQQI LSIISVKKTY KKSAPKGGSK TIRKPSQTKY SQTKSTKSTK STKSTNKKFV 850 GKSAKKTTKK TTKK

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.
[2]	"Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products." Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D. J. Virol. 80:11678-11685(2006) [PubMed: 16971431] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY653733 Genomic DNA. Translation: AAV50494.1.
RefSeq	YP_003986717.1. NC_014649.1.
3D structure databases
ProteinModelPortal	Q5UQB5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9924828.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 2 hits. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1P_MIMIV

Accession

Primary (citable) accession number: Q5UQB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 7, 2004
Last modified:	November 16, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program