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Q5UQB5

- TOP1P_MIMIV

UniProt

Q5UQB5 - TOP1P_MIMIV

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Protein

DNA topoisomerase 1 type prokaryotic

Gene

TOP1P

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactori

Mg2+By similarityNote: Binds two Mg(2+) per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium 1; catalyticBy similarity
Sitei32 – 321Interaction with DNABy similarity
Metal bindingi79 – 791Magnesium 1; catalyticBy similarity
Metal bindingi79 – 791Magnesium 2By similarity
Metal bindingi81 – 811Magnesium 2By similarity
Sitei134 – 1341Interaction with DNABy similarity
Sitei135 – 1351Interaction with DNABy similarity
Sitei138 – 1381Interaction with DNABy similarity
Sitei143 – 1431Interaction with DNABy similarity
Active sitei302 – 3021O-(5'-phospho-DNA)-tyrosine intermediateBy similarity
Sitei304 – 3041Interaction with DNABy similarity
Sitei505 – 5051Interaction with DNABy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 type prokaryotic (EC:5.99.1.2)
Gene namesi
Name:TOP1P
Ordered Locus Names:MIMI_L221
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134: Genome

Subcellular locationi

Virion 1 Publication

GO - Cellular componenti

  1. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 854854DNA topoisomerase 1 type prokaryoticPRO_0000145183Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5UQB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 110109ToprimAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni158 – 1636Interaction with DNABy similarity

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated
Contains 1 Toprim domain.Curated

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 3 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 2 hits.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 2 hits.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5UQB5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSILILLESP GKISKISSIL GKNYVVKASM GHFRDLDPKK MSIDFDNDFE
60 70 80 90 100
PVYIVTKPDV VKNLKSAMKN IDLVYLAADE DREGEAIAQS LYDVLKPSNY
110 120 130 140 150
KRLRFNAITK DAIMSAIKNA GDIDKNLVDA QKARRVLDRL FGYLISPILQ
160 170 180 190 200
RQIGGKLSAG RVQSVTVRII IDKENEIKNF INKNADSSYF KVSGTFNGAK
210 220 230 240 250
ATLHESNDKK PFDLETAYKG KTAQIALINS ENPNSKVVNF MKRCLKSQFF
260 270 280 290 300
IHSVEDKMTT RSPAPPFTTS TLQQEANRKF GMSIDSTMKT AQKLYEGGYI
310 320 330 340 350
TYMRTDSVEI SAEGHRDIKK IITDQYGADY YQKNLYKNKA ANSQEAHEAI
360 370 380 390 400
RPTHPELLTL EGEIEDAYQI KLYKLIWQRT IASQMKPAKI KVTIIQISIS
410 420 430 440 450
KYVEDKLNPF YYFQSQIETV VFPGFMKVYV ESIDDPDTDN QITKNFTGKI
460 470 480 490 500
PTVGSKVTME EIIARQEYMR PPPRYSEASL VKKLEELGIG RPSTYVNTIK
510 520 530 540 550
TIINREYVKI TDVPGIKKDI TIYSIKSENK KHIMEVYEDT DTILLGKENK
560 570 580 590 600
KIVPTNLGIT VNDFLMKYFP EFLDYKFTAN METDLDYVST GTKNWVDIVQ
610 620 630 640 650
DFYDKLKPIV DELSKQKGLS QSSERLLGED NDGNEITATK TKFGPVVRKK
660 670 680 690 700
IGDKYVYAKI KDPLTLDTIK LSDAIKLLEY PKNLGQYKGF DVLLQKGDYG
710 720 730 740 750
FYLSYNKENF SLGEIDDPED INLDTAIKAI EAKKANNIAE FNLTENGKKI
760 770 780 790 800
KAIVLNGKYG YYVQVTRNRI KKNYPIPKDL DPNNLTEQQI LSIISVKKTY
810 820 830 840 850
KKSAPKGGSK TIRKPSQTKY SQTKSTKSTK STKSTNKKFV GKSAKKTTKK

TTKK
Length:854
Mass (Da):96,946
Last modified:December 7, 2004 - v1
Checksum:i2D7361B66C8C0C0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAV50494.1.
RefSeqiYP_003986717.1. NC_014649.1.

Genome annotation databases

GeneIDi9924828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAV50494.1 .
RefSeqi YP_003986717.1. NC_014649.1.

3D structure databases

ProteinModelPortali Q5UQB5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9924828.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 3 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 2 hits.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 2 hits.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.
  2. "Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products."
    Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.
    J. Virol. 80:11678-11685(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTOP1P_MIMIV
AccessioniPrimary (citable) accession number: Q5UQB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3