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Q5UQB5 (TOP1P_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1 type prokaryotic

EC=5.99.1.2
Gene names
Name:TOP1P
Ordered Locus Names:MIMI_L221
OrganismAcanthamoeba polyphaga mimivirus (APMV) [Reference proteome]
Taxonomic identifier212035 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length854 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subcellular location

Virion HAMAP-Rule MF_00952.

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   Cellular componentVirion
   LigandDNA-binding
Magnesium
Metal-binding
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

virion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 854854DNA topoisomerase 1 type prokaryotic HAMAP-Rule MF_00952
PRO_0000145183

Regions

Domain2 – 110109Toprim
Region158 – 1636Interaction with DNA By similarity

Sites

Active site3021O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding81Magnesium 1; catalytic By similarity
Metal binding791Magnesium 1; catalytic By similarity
Metal binding791Magnesium 2 By similarity
Metal binding811Magnesium 2 By similarity
Site321Interaction with DNA By similarity
Site1341Interaction with DNA By similarity
Site1351Interaction with DNA By similarity
Site1381Interaction with DNA By similarity
Site1431Interaction with DNA By similarity
Site3041Interaction with DNA By similarity
Site5051Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5UQB5 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 2D7361B66C8C0C0C

FASTA85496,946
        10         20         30         40         50         60 
MSILILLESP GKISKISSIL GKNYVVKASM GHFRDLDPKK MSIDFDNDFE PVYIVTKPDV 

        70         80         90        100        110        120 
VKNLKSAMKN IDLVYLAADE DREGEAIAQS LYDVLKPSNY KRLRFNAITK DAIMSAIKNA 

       130        140        150        160        170        180 
GDIDKNLVDA QKARRVLDRL FGYLISPILQ RQIGGKLSAG RVQSVTVRII IDKENEIKNF 

       190        200        210        220        230        240 
INKNADSSYF KVSGTFNGAK ATLHESNDKK PFDLETAYKG KTAQIALINS ENPNSKVVNF 

       250        260        270        280        290        300 
MKRCLKSQFF IHSVEDKMTT RSPAPPFTTS TLQQEANRKF GMSIDSTMKT AQKLYEGGYI 

       310        320        330        340        350        360 
TYMRTDSVEI SAEGHRDIKK IITDQYGADY YQKNLYKNKA ANSQEAHEAI RPTHPELLTL 

       370        380        390        400        410        420 
EGEIEDAYQI KLYKLIWQRT IASQMKPAKI KVTIIQISIS KYVEDKLNPF YYFQSQIETV 

       430        440        450        460        470        480 
VFPGFMKVYV ESIDDPDTDN QITKNFTGKI PTVGSKVTME EIIARQEYMR PPPRYSEASL 

       490        500        510        520        530        540 
VKKLEELGIG RPSTYVNTIK TIINREYVKI TDVPGIKKDI TIYSIKSENK KHIMEVYEDT 

       550        560        570        580        590        600 
DTILLGKENK KIVPTNLGIT VNDFLMKYFP EFLDYKFTAN METDLDYVST GTKNWVDIVQ 

       610        620        630        640        650        660 
DFYDKLKPIV DELSKQKGLS QSSERLLGED NDGNEITATK TKFGPVVRKK IGDKYVYAKI 

       670        680        690        700        710        720 
KDPLTLDTIK LSDAIKLLEY PKNLGQYKGF DVLLQKGDYG FYLSYNKENF SLGEIDDPED 

       730        740        750        760        770        780 
INLDTAIKAI EAKKANNIAE FNLTENGKKI KAIVLNGKYG YYVQVTRNRI KKNYPIPKDL 

       790        800        810        820        830        840 
DPNNLTEQQI LSIISVKKTY KKSAPKGGSK TIRKPSQTKY SQTKSTKSTK STKSTNKKFV 

       850 
GKSAKKTTKK TTKK 

« Hide

References

[1]"The 1.2-megabase genome sequence of Mimivirus."
Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M.
Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rowbotham-Bradford.
[2]"Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products."
Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.
J. Virol. 80:11678-11685(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY653733 Genomic DNA. Translation: AAV50494.1.
RefSeqYP_003986717.1. NC_014649.1.

3D structure databases

ProteinModelPortalQ5UQB5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9924828.

Family and domain databases

Gene3D1.10.290.10. 1 hit.
1.10.460.10. 3 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 2 hits.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 2 hits.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1P_MIMIV
AccessionPrimary (citable) accession number: Q5UQB5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: July 9, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families