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Q5UQB5

- TOP1P_MIMIV

UniProt

Q5UQB5 - TOP1P_MIMIV

Protein

DNA topoisomerase 1 type prokaryotic

Gene

TOP1P

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

    Cofactori

    Magnesium. Binds two Mg2+ per subunit By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81Magnesium 1; catalyticBy similarity
    Sitei32 – 321Interaction with DNABy similarity
    Metal bindingi79 – 791Magnesium 1; catalyticBy similarity
    Metal bindingi79 – 791Magnesium 2By similarity
    Metal bindingi81 – 811Magnesium 2By similarity
    Sitei134 – 1341Interaction with DNABy similarity
    Sitei135 – 1351Interaction with DNABy similarity
    Sitei138 – 1381Interaction with DNABy similarity
    Sitei143 – 1431Interaction with DNABy similarity
    Active sitei302 – 3021O-(5'-phospho-DNA)-tyrosine intermediateBy similarity
    Sitei304 – 3041Interaction with DNABy similarity
    Sitei505 – 5051Interaction with DNABy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1 type prokaryotic (EC:5.99.1.2)
    Gene namesi
    Name:TOP1P
    Ordered Locus Names:MIMI_L221
    OrganismiAcanthamoeba polyphaga mimivirus (APMV)
    Taxonomic identifieri212035 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
    Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
    ProteomesiUP000001134: Genome

    Subcellular locationi

    Virion 1 Publication

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 854854DNA topoisomerase 1 type prokaryoticPRO_0000145183Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ5UQB5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 110109ToprimAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni158 – 1636Interaction with DNABy similarity

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.Curated
    Contains 1 Toprim domain.Curated

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 3 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 2 hits.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 2 hits.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5UQB5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSILILLESP GKISKISSIL GKNYVVKASM GHFRDLDPKK MSIDFDNDFE    50
    PVYIVTKPDV VKNLKSAMKN IDLVYLAADE DREGEAIAQS LYDVLKPSNY 100
    KRLRFNAITK DAIMSAIKNA GDIDKNLVDA QKARRVLDRL FGYLISPILQ 150
    RQIGGKLSAG RVQSVTVRII IDKENEIKNF INKNADSSYF KVSGTFNGAK 200
    ATLHESNDKK PFDLETAYKG KTAQIALINS ENPNSKVVNF MKRCLKSQFF 250
    IHSVEDKMTT RSPAPPFTTS TLQQEANRKF GMSIDSTMKT AQKLYEGGYI 300
    TYMRTDSVEI SAEGHRDIKK IITDQYGADY YQKNLYKNKA ANSQEAHEAI 350
    RPTHPELLTL EGEIEDAYQI KLYKLIWQRT IASQMKPAKI KVTIIQISIS 400
    KYVEDKLNPF YYFQSQIETV VFPGFMKVYV ESIDDPDTDN QITKNFTGKI 450
    PTVGSKVTME EIIARQEYMR PPPRYSEASL VKKLEELGIG RPSTYVNTIK 500
    TIINREYVKI TDVPGIKKDI TIYSIKSENK KHIMEVYEDT DTILLGKENK 550
    KIVPTNLGIT VNDFLMKYFP EFLDYKFTAN METDLDYVST GTKNWVDIVQ 600
    DFYDKLKPIV DELSKQKGLS QSSERLLGED NDGNEITATK TKFGPVVRKK 650
    IGDKYVYAKI KDPLTLDTIK LSDAIKLLEY PKNLGQYKGF DVLLQKGDYG 700
    FYLSYNKENF SLGEIDDPED INLDTAIKAI EAKKANNIAE FNLTENGKKI 750
    KAIVLNGKYG YYVQVTRNRI KKNYPIPKDL DPNNLTEQQI LSIISVKKTY 800
    KKSAPKGGSK TIRKPSQTKY SQTKSTKSTK STKSTNKKFV GKSAKKTTKK 850
    TTKK 854
    Length:854
    Mass (Da):96,946
    Last modified:December 7, 2004 - v1
    Checksum:i2D7361B66C8C0C0C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY653733 Genomic DNA. Translation: AAV50494.1.
    RefSeqiYP_003986717.1. NC_014649.1.

    Genome annotation databases

    GeneIDi9924828.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY653733 Genomic DNA. Translation: AAV50494.1 .
    RefSeqi YP_003986717.1. NC_014649.1.

    3D structure databases

    ProteinModelPortali Q5UQB5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 9924828.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 3 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 2 hits.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 2 hits.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Rowbotham-Bradford.
    2. "Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products."
      Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.
      J. Virol. 80:11678-11685(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiTOP1P_MIMIV
    AccessioniPrimary (citable) accession number: Q5UQB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3