Q5UQ88 (UBC3_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin-conjugating enzyme E2 R521
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein
Ubiquitin-protein ligase

Gene names

Ordered Locus Names:

MIMI_R521

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Reference proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	1441 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA protection Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1441	1441	Probable ubiquitin-conjugating enzyme E2 R521		PRO_0000243982
Regions
	Transmembrane	20 – 40	21	Helical; Potential
	Coiled coil	63 – 89	27	Potential
	Coiled coil	340 – 368	29	Potential
Sites
	Active site	1306	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UQ88 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 0F605583124D4F0A	FASTA	1,441	165,046
	10 20 30 40 50 60 MHDYLGSFYN PLTLQKKLKY IHHIIINYIT NSILYFFLIM QSKNNPKHNS MKFKEQTESP 70 80 90 100 110 120 ILNQSKLVNT LDIIKDEINK WEEKNTDKDI KIVSIDNGKI VLSMVYGKQH IIEILCPKDY 130 140 150 160 170 180 PNVKSGFSCK EIKTVNTIPL SFISQANNQL KSKKNLSVHR IISHLSTTFQ NYKKALKSKV 190 200 210 220 230 240 SKDKMKDKSE SNSEHEQESK SVVSNEIPSE VSIDSKLTKD INFINDLIKE ANKCSNDVPS 250 260 270 280 290 300 DSDETHQEVD DRPLTEEVVV PDPTRIVRRR NSKLQTGSTK IKIFGKSKNS GPSSSKTSIS 310 320 330 340 350 360 SMSKVEELED KNPYLQEQDI MSIIQDEWNK VQESKSICPT TNEDNNDLDN LINEVERLVQ 370 380 390 400 410 420 ETKDQETKDQ ETKDQKEIWA SSLTIEEPNG ENFEEFSWEV PNETSQITVV EPVQEATEEP 430 440 450 460 470 480 VQNVTEELIQ LVADEPVQET VKDSVQEVAE ESVQETTVEA VQEIAEESVQ QVVEESVQET 490 500 510 520 530 540 TVEPVQEVAE EAVQQVVEES VQETTVEPVQ EVAEEPVQQE VAEEPVQQEV AEEPVQQEVA 550 560 570 580 590 600 EEPVQEVAEE PVQKATEELV QQEVAEDIVK LDVTVQNDFS DHSDSPEPSD SSDSEEEITN 610 620 630 640 650 660 SNNLGRYFKI YDPTTGKTTG VYVGKTPTQA ANKAFIKTFS NDNTGNKKEF YLQEYTGNKP 670 680 690 700 710 720 GKIYKYEGTR QKLNQPQKIS IPLYGDGQYK TITYNYKNTV VKKNVPDSIK NPPKTIKKSI 730 740 750 760 770 780 KPVKKSIKPV KKLSTTKLDS QTNSVKEKSE NKDIINYNDT GDKYGLYIDF GKFFKNSKIP 790 800 810 820 830 840 FDLEKLRDNA LKLSNQENQD DNYSTMKLRN FKNNNAVNLM INDFTKLYND GVKNGYNIEP 850 860 870 880 890 900 VNNNIYDLDI LLSSNFLDKD SVLYQDMINL KIDHIKINIK INHKMYPFYP PQVSLIRPTI 910 920 930 940 950 960 ENNVAAIIAT IDYLFANKWN PMISIVNIVN DIRNILNKYG ALDEKKYQNN LDPIFHDLVE 970 980 990 1000 1010 1020 LSLLTGTQCS MYQSDQKVID LSNNSTNEKQ SKYWKKGTGF GHSGLSDWDF NQTKENIKNR 1030 1040 1050 1060 1070 1080 ELKIYQCLRK IVVKLTKIIL GKNQVDVINI LKESCFIPYL KLVFIDGSLF DLVKDLSYFE 1090 1100 1110 1120 1130 1140 LVLNSMRILT KEYLPLFLHK YNDKSLLEVL DQFNKDCHSY LNTLKNIKES DCQNEIDIIE 1150 1160 1170 1180 1190 1200 NFMSFYKRLS TSIEKFNEIT ESNNKEEVIS NDVKDLYKIT LGNEVFQEYN LDLNKFANML 1210 1220 1230 1240 1250 1260 TNDTKKEGLI HKDALKAISR ELLSHSKNLP VEYGSSIYYR YSPENIRYHE FIITGPEDSP 1270 1280 1290 1300 1310 1320 YDSGCFHFRM YNPSAYPNTS PFVSMTTTGH GSVRFNPNLY ADGKVCLSIL GTWRGQAGES 1330 1340 1350 1360 1370 1380 WIPGVSSMLQ VMISIQSLVL ISEPYFNEPG YESSRGTDKG NKLSTEYNQK VRFNCMKWAM 1390 1400 1410 1420 1430 1440 IDVIKNPVPG FESMIKKHFS IKAPHIKQVC QTWINEAPDN NKSEYQKLYD ELIGLLDSLV A

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY653733 Genomic DNA. Translation: AAV50785.1.
3D structure databases
ProteinModelPortal	Q5UQ88.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 2 hits.
InterPro	IPR008674. MC1. IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF102875. MC1. 1 hit. SSF54495. UBQ-conjugat/RWD-like. 2 hits.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC3_MIMIV

Accession

Primary (citable) accession number: Q5UQ88

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	December 7, 2004
Last modified:	March 6, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program