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Q5UQ88 (UBC3_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ubiquitin-conjugating enzyme E2 R521

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene names
Ordered Locus Names:MIMI_R521
OrganismAcanthamoeba polyphaga mimivirus (APMV) [Reference proteome]
Taxonomic identifier212035 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length1441 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentMembrane
   DomainCoiled coil
Transmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA protection

Inferred from electronic annotation. Source: InterPro

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14411441Probable ubiquitin-conjugating enzyme E2 R521
PRO_0000243982

Regions

Transmembrane20 – 4021Helical; Potential
Coiled coil63 – 8927 Potential
Coiled coil340 – 36829 Potential

Sites

Active site13061Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5UQ88 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 0F605583124D4F0A

FASTA1,441165,046
        10         20         30         40         50         60 
MHDYLGSFYN PLTLQKKLKY IHHIIINYIT NSILYFFLIM QSKNNPKHNS MKFKEQTESP 

        70         80         90        100        110        120 
ILNQSKLVNT LDIIKDEINK WEEKNTDKDI KIVSIDNGKI VLSMVYGKQH IIEILCPKDY 

       130        140        150        160        170        180 
PNVKSGFSCK EIKTVNTIPL SFISQANNQL KSKKNLSVHR IISHLSTTFQ NYKKALKSKV 

       190        200        210        220        230        240 
SKDKMKDKSE SNSEHEQESK SVVSNEIPSE VSIDSKLTKD INFINDLIKE ANKCSNDVPS 

       250        260        270        280        290        300 
DSDETHQEVD DRPLTEEVVV PDPTRIVRRR NSKLQTGSTK IKIFGKSKNS GPSSSKTSIS 

       310        320        330        340        350        360 
SMSKVEELED KNPYLQEQDI MSIIQDEWNK VQESKSICPT TNEDNNDLDN LINEVERLVQ 

       370        380        390        400        410        420 
ETKDQETKDQ ETKDQKEIWA SSLTIEEPNG ENFEEFSWEV PNETSQITVV EPVQEATEEP 

       430        440        450        460        470        480 
VQNVTEELIQ LVADEPVQET VKDSVQEVAE ESVQETTVEA VQEIAEESVQ QVVEESVQET 

       490        500        510        520        530        540 
TVEPVQEVAE EAVQQVVEES VQETTVEPVQ EVAEEPVQQE VAEEPVQQEV AEEPVQQEVA 

       550        560        570        580        590        600 
EEPVQEVAEE PVQKATEELV QQEVAEDIVK LDVTVQNDFS DHSDSPEPSD SSDSEEEITN 

       610        620        630        640        650        660 
SNNLGRYFKI YDPTTGKTTG VYVGKTPTQA ANKAFIKTFS NDNTGNKKEF YLQEYTGNKP 

       670        680        690        700        710        720 
GKIYKYEGTR QKLNQPQKIS IPLYGDGQYK TITYNYKNTV VKKNVPDSIK NPPKTIKKSI 

       730        740        750        760        770        780 
KPVKKSIKPV KKLSTTKLDS QTNSVKEKSE NKDIINYNDT GDKYGLYIDF GKFFKNSKIP 

       790        800        810        820        830        840 
FDLEKLRDNA LKLSNQENQD DNYSTMKLRN FKNNNAVNLM INDFTKLYND GVKNGYNIEP 

       850        860        870        880        890        900 
VNNNIYDLDI LLSSNFLDKD SVLYQDMINL KIDHIKINIK INHKMYPFYP PQVSLIRPTI 

       910        920        930        940        950        960 
ENNVAAIIAT IDYLFANKWN PMISIVNIVN DIRNILNKYG ALDEKKYQNN LDPIFHDLVE 

       970        980        990       1000       1010       1020 
LSLLTGTQCS MYQSDQKVID LSNNSTNEKQ SKYWKKGTGF GHSGLSDWDF NQTKENIKNR 

      1030       1040       1050       1060       1070       1080 
ELKIYQCLRK IVVKLTKIIL GKNQVDVINI LKESCFIPYL KLVFIDGSLF DLVKDLSYFE 

      1090       1100       1110       1120       1130       1140 
LVLNSMRILT KEYLPLFLHK YNDKSLLEVL DQFNKDCHSY LNTLKNIKES DCQNEIDIIE 

      1150       1160       1170       1180       1190       1200 
NFMSFYKRLS TSIEKFNEIT ESNNKEEVIS NDVKDLYKIT LGNEVFQEYN LDLNKFANML 

      1210       1220       1230       1240       1250       1260 
TNDTKKEGLI HKDALKAISR ELLSHSKNLP VEYGSSIYYR YSPENIRYHE FIITGPEDSP 

      1270       1280       1290       1300       1310       1320 
YDSGCFHFRM YNPSAYPNTS PFVSMTTTGH GSVRFNPNLY ADGKVCLSIL GTWRGQAGES 

      1330       1340       1350       1360       1370       1380 
WIPGVSSMLQ VMISIQSLVL ISEPYFNEPG YESSRGTDKG NKLSTEYNQK VRFNCMKWAM 

      1390       1400       1410       1420       1430       1440 
IDVIKNPVPG FESMIKKHFS IKAPHIKQVC QTWINEAPDN NKSEYQKLYD ELIGLLDSLV 


A 

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References

[1]"The 1.2-megabase genome sequence of Mimivirus."
Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M.
Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rowbotham-Bradford.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY653733 Genomic DNA. Translation: AAV50785.1.

3D structure databases

ProteinModelPortalQ5UQ88.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 2 hits.
InterProIPR008674. MC1.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF102875. SSF102875. 1 hit.
SSF54495. SSF54495. 2 hits.
PROSITEPS50127. UBIQUITIN_CONJUGAT_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC3_MIMIV
AccessionPrimary (citable) accession number: Q5UQ88
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: December 7, 2004
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways