Q5UQ40 (UBC4_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable bifunctional E2/E3 enzyme R795

Including the following 2 domains:

1. E3 ubiquitin-protein ligase
   EC=6.3.2.-
2. Ubiquitin-conjugating enzyme E2
   EC=6.3.2.19

Gene names

Ordered Locus Names:

MIMI_R795

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Reference proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	1297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Also act as a E3 ubiquitin-protein ligase Potential.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Domain	The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme By similarity.
Sequence similarities	In the C-terminal section; belongs to the ubiquitin-conjugating enzyme family. Contains 1 RING-type zinc finger. Contains 1 VWFA domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	ubiquitin ligase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1297	1297	Probable bifunctional E2/E3 enzyme R795		PRO_0000309555
Regions
	Domain	899 – 1082	184	VWFA
	Zinc finger	74 – 128	55	RING-type; atypical
Sites
	Active site	1217	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UQ40 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: BE177D02F78F6685	FASTA	1,297	150,351
	10 20 30 40 50 60 MEQIVTFGGI TIDNVSPDHI QQLYSVKLNG TSNNEEIPTE FPNIIQSVCS CDKHVVELTM 70 80 90 100 110 120 DVFKLVDTTE NSSCAICRYQ ENEPCIEHKS SESNTKCPIA QSVSCSHSFH ACCISRWLHT 130 140 150 160 170 180 KKTCPLCNIE WQLIGANNDS VVIYFDDKNQ EFKLSDNLVE DIGRQFGIDM DNYIVCKNKS 190 200 210 220 230 240 PVTEYKNSTY ALCTRDRHNS QNGQTNLKII CEFDSKTENL FIKYSTTIEE LRKLVSQTFD 250 260 270 280 290 300 LFKDQVKLIY NNIEISKDFD NLTVFNIGIK NDSNLAVECY KSFTYDMEIT NNFMVLYVPD 310 320 330 340 350 360 TFNTTNSSNN IQSVVSGSIA WIPQPFIDNV TNKDLRCLLS SLYILVKKVN LNTELIQSVT 370 380 390 400 410 420 DRFEKYMELY GMHYRQIKLA KDSLRCLLEM NHFNNKDRMI LSCTFYELID RIQRDNNINK 430 440 450 460 470 480 NPLLSSNLIC NLILSDKQVN EQTKINWKFL AKDVRITKVF NIYSPLVLTN SVPPLLTLNK 490 500 510 520 530 540 NLDVVVFTGK GKDVSLPIIL YDTLTNSETD VNAAELGKIV SDKGDLMMVD DRIYEEAIMV 550 560 570 580 590 600 CIDTSNSMSK ASDFDEDIKL KRSSIIETKN RFYEILKTES HSSPQESDIR QLTNTIIWFI 610 620 630 640 650 660 THPNFEDWYR KLYSQELIRS IACFEQKVYP NFAMMLVKYP WFFNKLLTSK KVVVNNTWYS 670 680 690 700 710 720 FIRNESNSNY NETKYSKEPL QEFLCPISHE IMEHPVVAKD GFTYEKSNIL KWFENKSTSP 730 740 750 760 770 780 MTNEKISKKI CDNKVLKSII RDWKENNTVI EQSDKLSVTI KLPDPWNETV IHYNETDNIW 790 800 810 820 830 840 DLIYQIYHIT GLSHDQYKLT SNYWAIDKSS LIKQISSKIK IHPFEKKMVD VKICDKTYFF 850 860 870 880 890 900 GTENTMTVSS FYTVGNLLYK LKSRKYHRYA VWYGLKDSGD GFQRGTILSP HDKLIDYSEM 910 920 930 940 950 960 TLEIHSSNRY KTPKGNHLSR LDVVKKLFDA YINRSIAYSF NTAIGLMSFS DKSVLECAIS 970 980 990 1000 1010 1020 PFYESFREKV NELDTSGATA LYECLKDSIE NLIEWKNADL ENRSKAKLRI ICLTDGKDTG 1030 1040 1050 1060 1070 1080 LDKFKNTVKH KSQYHNVTID CILIGSDYDN YLGKIGEKTN GYVFNPSTIK YALDIMELET 1090 1100 1110 1120 1130 1140 MISSTNRKTI FYHNTIDEKT IPPIINPTKK LHAKAISPME IISKTNENTK LSQKLIRVQR 1150 1160 1170 1180 1190 1200 EIVDVMKNQH PDIDVYINEQ DISFWKIVFK GPDSTPYKNG TWLAYIQFTE EYPNIAPNIR 1210 1220 1230 1240 1250 1260 FVTPIKHCNI NNYGRVCHSI LDRNYTPNVK ISLILQCIYG LLLNPDVNDP LDTNLAMIYY 1270 1280 1290 DANGLYEAQI IDYVNKFALK SREEWNQELA KNQFKNW

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY653733 Genomic DNA. Translation: AAV51055.1.
3D structure databases
HSSP	HSSP built from PDB template 1T1H based on UniProtKB Q8VZ40.
ProteinModelPortal	Q5UQ40.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit. 3.30.40.10. 2 hits.
InterPro	IPR003613. Ubox_domain. IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. IPR002035. VWF_A. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR024766. Znf_RING_H2. [Graphical view]
Pfam	PF04564. U-box. 1 hit. PF00179. UQ_con. 1 hit. PF12678. zf-rbx1. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00504. Ubox. 1 hit. SM00327. VWA. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. PS50234. VWFA. 1 hit. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC4_MIMIV

Accession

Primary (citable) accession number: Q5UQ40

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	December 7, 2004
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program