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Q5UQ40 (UBC4_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable bifunctional E2/E3 enzyme R795

Including the following 2 domains:

  1. E3 ubiquitin-protein ligase
    EC=6.3.2.-
  2. Ubiquitin-conjugating enzyme E2
    EC=6.3.2.19
Gene names
Ordered Locus Names:MIMI_R795
OrganismAcanthamoeba polyphaga mimivirus (APMV) [Reference proteome]
Taxonomic identifier212035 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length1297 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Also act as a E3 ubiquitin-protein ligase Potential.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Domain

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme By similarity.

Sequence similarities

In the C-terminal section; belongs to the ubiquitin-conjugating enzyme family.

Contains 1 RING-type zinc finger.

Contains 1 U-box domain.

Contains 1 VWFA domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12971297Probable bifunctional E2/E3 enzyme R795
PRO_0000309555

Regions

Domain678 – 75073U-box
Domain899 – 1082184VWFA
Zinc finger74 – 12855RING-type; atypical

Sites

Active site12171Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5UQ40 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: BE177D02F78F6685

FASTA1,297150,351
        10         20         30         40         50         60 
MEQIVTFGGI TIDNVSPDHI QQLYSVKLNG TSNNEEIPTE FPNIIQSVCS CDKHVVELTM 

        70         80         90        100        110        120 
DVFKLVDTTE NSSCAICRYQ ENEPCIEHKS SESNTKCPIA QSVSCSHSFH ACCISRWLHT 

       130        140        150        160        170        180 
KKTCPLCNIE WQLIGANNDS VVIYFDDKNQ EFKLSDNLVE DIGRQFGIDM DNYIVCKNKS 

       190        200        210        220        230        240 
PVTEYKNSTY ALCTRDRHNS QNGQTNLKII CEFDSKTENL FIKYSTTIEE LRKLVSQTFD 

       250        260        270        280        290        300 
LFKDQVKLIY NNIEISKDFD NLTVFNIGIK NDSNLAVECY KSFTYDMEIT NNFMVLYVPD 

       310        320        330        340        350        360 
TFNTTNSSNN IQSVVSGSIA WIPQPFIDNV TNKDLRCLLS SLYILVKKVN LNTELIQSVT 

       370        380        390        400        410        420 
DRFEKYMELY GMHYRQIKLA KDSLRCLLEM NHFNNKDRMI LSCTFYELID RIQRDNNINK 

       430        440        450        460        470        480 
NPLLSSNLIC NLILSDKQVN EQTKINWKFL AKDVRITKVF NIYSPLVLTN SVPPLLTLNK 

       490        500        510        520        530        540 
NLDVVVFTGK GKDVSLPIIL YDTLTNSETD VNAAELGKIV SDKGDLMMVD DRIYEEAIMV 

       550        560        570        580        590        600 
CIDTSNSMSK ASDFDEDIKL KRSSIIETKN RFYEILKTES HSSPQESDIR QLTNTIIWFI 

       610        620        630        640        650        660 
THPNFEDWYR KLYSQELIRS IACFEQKVYP NFAMMLVKYP WFFNKLLTSK KVVVNNTWYS 

       670        680        690        700        710        720 
FIRNESNSNY NETKYSKEPL QEFLCPISHE IMEHPVVAKD GFTYEKSNIL KWFENKSTSP 

       730        740        750        760        770        780 
MTNEKISKKI CDNKVLKSII RDWKENNTVI EQSDKLSVTI KLPDPWNETV IHYNETDNIW 

       790        800        810        820        830        840 
DLIYQIYHIT GLSHDQYKLT SNYWAIDKSS LIKQISSKIK IHPFEKKMVD VKICDKTYFF 

       850        860        870        880        890        900 
GTENTMTVSS FYTVGNLLYK LKSRKYHRYA VWYGLKDSGD GFQRGTILSP HDKLIDYSEM 

       910        920        930        940        950        960 
TLEIHSSNRY KTPKGNHLSR LDVVKKLFDA YINRSIAYSF NTAIGLMSFS DKSVLECAIS 

       970        980        990       1000       1010       1020 
PFYESFREKV NELDTSGATA LYECLKDSIE NLIEWKNADL ENRSKAKLRI ICLTDGKDTG 

      1030       1040       1050       1060       1070       1080 
LDKFKNTVKH KSQYHNVTID CILIGSDYDN YLGKIGEKTN GYVFNPSTIK YALDIMELET 

      1090       1100       1110       1120       1130       1140 
MISSTNRKTI FYHNTIDEKT IPPIINPTKK LHAKAISPME IISKTNENTK LSQKLIRVQR 

      1150       1160       1170       1180       1190       1200 
EIVDVMKNQH PDIDVYINEQ DISFWKIVFK GPDSTPYKNG TWLAYIQFTE EYPNIAPNIR 

      1210       1220       1230       1240       1250       1260 
FVTPIKHCNI NNYGRVCHSI LDRNYTPNVK ISLILQCIYG LLLNPDVNDP LDTNLAMIYY 

      1270       1280       1290 
DANGLYEAQI IDYVNKFALK SREEWNQELA KNQFKNW 

« Hide

References

[1]"The 1.2-megabase genome sequence of Mimivirus."
Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M.
Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rowbotham-Bradford.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY653733 Genomic DNA. Translation: AAV51055.1.

3D structure databases

ProteinModelPortalQ5UQ40.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
3.30.40.10. 2 hits.
3.40.50.410. 1 hit.
InterProIPR029071. Ubiquitin-rel_dom.
IPR003613. Ubox_domain.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR002035. VWF_A.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamPF04564. U-box. 1 hit.
PF00179. UQ_con. 1 hit.
PF12678. zf-rbx1. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
SM00504. Ubox. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEPS51698. U_BOX. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
PS50234. VWFA. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC4_MIMIV
AccessionPrimary (citable) accession number: Q5UQ40
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 7, 2004
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways