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Protein

Probable bifunctional E2/E3 enzyme R795

Gene

MIMI_R795

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Also act as a E3 ubiquitin-protein ligase (Potential).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1217 – 12171Glycyl thioester intermediatePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri74 – 12855RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional E2/E3 enzyme R795
Including the following 2 domains:
E3 ubiquitin-protein ligase (EC:6.3.2.-)
Ubiquitin-conjugating enzyme E2 (EC:6.3.2.19)
Gene namesi
Ordered Locus Names:MIMI_R795
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12971297Probable bifunctional E2/E3 enzyme R795PRO_0000309555Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5UQ40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini678 – 75073U-boxAdd
BLAST
Domaini899 – 1082184VWFAPROSITE-ProRule annotationAdd
BLAST

Domaini

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the ubiquitin-conjugating enzyme family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 U-box domain.Curated
Contains 1 VWFA domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri74 – 12855RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
3.30.40.10. 2 hits.
3.40.50.410. 1 hit.
InterProiIPR029071. Ubiquitin-rel_dom.
IPR003613. Ubox_domain.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR002035. VWF_A.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamiPF04564. U-box. 1 hit.
PF00179. UQ_con. 1 hit.
PF12678. zf-rbx1. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00504. Ubox. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS51698. U_BOX. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
PS50234. VWFA. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5UQ40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQIVTFGGI TIDNVSPDHI QQLYSVKLNG TSNNEEIPTE FPNIIQSVCS
60 70 80 90 100
CDKHVVELTM DVFKLVDTTE NSSCAICRYQ ENEPCIEHKS SESNTKCPIA
110 120 130 140 150
QSVSCSHSFH ACCISRWLHT KKTCPLCNIE WQLIGANNDS VVIYFDDKNQ
160 170 180 190 200
EFKLSDNLVE DIGRQFGIDM DNYIVCKNKS PVTEYKNSTY ALCTRDRHNS
210 220 230 240 250
QNGQTNLKII CEFDSKTENL FIKYSTTIEE LRKLVSQTFD LFKDQVKLIY
260 270 280 290 300
NNIEISKDFD NLTVFNIGIK NDSNLAVECY KSFTYDMEIT NNFMVLYVPD
310 320 330 340 350
TFNTTNSSNN IQSVVSGSIA WIPQPFIDNV TNKDLRCLLS SLYILVKKVN
360 370 380 390 400
LNTELIQSVT DRFEKYMELY GMHYRQIKLA KDSLRCLLEM NHFNNKDRMI
410 420 430 440 450
LSCTFYELID RIQRDNNINK NPLLSSNLIC NLILSDKQVN EQTKINWKFL
460 470 480 490 500
AKDVRITKVF NIYSPLVLTN SVPPLLTLNK NLDVVVFTGK GKDVSLPIIL
510 520 530 540 550
YDTLTNSETD VNAAELGKIV SDKGDLMMVD DRIYEEAIMV CIDTSNSMSK
560 570 580 590 600
ASDFDEDIKL KRSSIIETKN RFYEILKTES HSSPQESDIR QLTNTIIWFI
610 620 630 640 650
THPNFEDWYR KLYSQELIRS IACFEQKVYP NFAMMLVKYP WFFNKLLTSK
660 670 680 690 700
KVVVNNTWYS FIRNESNSNY NETKYSKEPL QEFLCPISHE IMEHPVVAKD
710 720 730 740 750
GFTYEKSNIL KWFENKSTSP MTNEKISKKI CDNKVLKSII RDWKENNTVI
760 770 780 790 800
EQSDKLSVTI KLPDPWNETV IHYNETDNIW DLIYQIYHIT GLSHDQYKLT
810 820 830 840 850
SNYWAIDKSS LIKQISSKIK IHPFEKKMVD VKICDKTYFF GTENTMTVSS
860 870 880 890 900
FYTVGNLLYK LKSRKYHRYA VWYGLKDSGD GFQRGTILSP HDKLIDYSEM
910 920 930 940 950
TLEIHSSNRY KTPKGNHLSR LDVVKKLFDA YINRSIAYSF NTAIGLMSFS
960 970 980 990 1000
DKSVLECAIS PFYESFREKV NELDTSGATA LYECLKDSIE NLIEWKNADL
1010 1020 1030 1040 1050
ENRSKAKLRI ICLTDGKDTG LDKFKNTVKH KSQYHNVTID CILIGSDYDN
1060 1070 1080 1090 1100
YLGKIGEKTN GYVFNPSTIK YALDIMELET MISSTNRKTI FYHNTIDEKT
1110 1120 1130 1140 1150
IPPIINPTKK LHAKAISPME IISKTNENTK LSQKLIRVQR EIVDVMKNQH
1160 1170 1180 1190 1200
PDIDVYINEQ DISFWKIVFK GPDSTPYKNG TWLAYIQFTE EYPNIAPNIR
1210 1220 1230 1240 1250
FVTPIKHCNI NNYGRVCHSI LDRNYTPNVK ISLILQCIYG LLLNPDVNDP
1260 1270 1280 1290
LDTNLAMIYY DANGLYEAQI IDYVNKFALK SREEWNQELA KNQFKNW
Length:1,297
Mass (Da):150,351
Last modified:December 7, 2004 - v1
Checksum:iBE177D02F78F6685
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAV51055.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAV51055.1.

3D structure databases

ProteinModelPortaliQ5UQ40.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
3.30.40.10. 2 hits.
3.40.50.410. 1 hit.
InterProiIPR029071. Ubiquitin-rel_dom.
IPR003613. Ubox_domain.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR002035. VWF_A.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR024766. Znf_RING_H2.
[Graphical view]
PfamiPF04564. U-box. 1 hit.
PF00179. UQ_con. 1 hit.
PF12678. zf-rbx1. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00504. Ubox. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS51698. U_BOX. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
PS50234. VWFA. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.

Entry informationi

Entry nameiUBC4_MIMIV
AccessioniPrimary (citable) accession number: Q5UQ40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 7, 2004
Last modified: March 4, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.