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Q5UQ00

- FPG_MIMIV

UniProt

Q5UQ00 - FPG_MIMIV

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Protein
Probable formamidopyrimidine-DNA glycosylase
Gene
MIMI_L315
Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNA By similarity
Active sitei3 – 31Proton donor By similarity
Active sitei58 – 581Proton donor; for beta-elimination activity By similarity
Active sitei277 – 2771Proton donor; for delta-elimination activity By similarity

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. oxidized purine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-EC
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable formamidopyrimidine-DNA glycosylase (EC:3.2.2.23)
Short name:
Fapy-DNA glycosylase
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Ordered Locus Names:MIMI_L315
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host By similarity
Chaini2 – 287286Probable formamidopyrimidine-DNA glycosylase
PRO_0000170892Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1815
Beta strandi22 – 287
Helixi32 – 354
Helixi41 – 455
Beta strandi49 – 5810
Beta strandi61 – 688
Beta strandi71 – 8111
Turni83 – 853
Beta strandi86 – 916
Beta strandi96 – 1027
Helixi103 – 1053
Beta strandi107 – 1126
Beta strandi118 – 1236
Helixi125 – 1328
Turni139 – 1413
Helixi147 – 1526
Helixi157 – 1626
Beta strandi164 – 1674
Helixi173 – 18210
Helixi191 – 1933
Helixi196 – 21520
Beta strandi219 – 2213
Helixi223 – 2253
Helixi226 – 2294
Beta strandi230 – 2323
Beta strandi244 – 2474
Beta strandi259 – 2613
Beta strandi265 – 2684
Helixi273 – 2753
Beta strandi278 – 2814
Turni283 – 2853

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A42X-ray2.60A1-287[»]
3A45X-ray2.30A/B1-287[»]
3A46X-ray2.20A/B1-287[»]
3VK7X-ray2.10A/B1-287[»]
3VK8X-ray2.00A/B1-287[»]
4NRWX-ray2.84A/B2-287[»]
ProteinModelPortaliQ5UQ00.

Miscellaneous databases

EvolutionaryTraceiQ5UQ00.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5UQ00-1 [UniParc]FASTAAdd to Basket

« Hide

MPEGPEVALT ADILEKYFKG KTLEYIDFIS GRYSKSEPEG YDDFIANLPL    50
KVSNVDTKGK FLWFELFDPN DKSNKWYIWN TFGLTGMWSL FEAKYTRAVL 100
SFDNELMAYF SDMRNFGTFK FSNSEKELKR KLNELGPDFL KNDDIDISKI 150
KKYKQPIVAL LMDQKKIGSG LGNYLVAEIL YRAKIDPHKL GSNLTDQEIE 200
NLWYWIKYET KLAYDSNHIG YMVNLENESS KIGRKNYHPN IHPTEKEFDF 250
LVYRKKKDPN GNKVIADKII GSGKNKRTTY WAPAIQK 287
Length:287
Mass (Da):33,463
Last modified:January 23, 2007 - v3
Checksum:i745EE3385D9581AB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAV50585.1.
RefSeqiYP_003986818.1. NC_014649.1.

Genome annotation databases

GeneIDi9924932.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAV50585.1 .
RefSeqi YP_003986818.1. NC_014649.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3A42 X-ray 2.60 A 1-287 [» ]
3A45 X-ray 2.30 A/B 1-287 [» ]
3A46 X-ray 2.20 A/B 1-287 [» ]
3VK7 X-ray 2.10 A/B 1-287 [» ]
3VK8 X-ray 2.00 A/B 1-287 [» ]
4NRW X-ray 2.84 A/B 2-287 [» ]
ProteinModelPortali Q5UQ00.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9924932.

Miscellaneous databases

EvolutionaryTracei Q5UQ00.

Family and domain databases

InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.

Entry informationi

Entry nameiFPG_MIMIV
AccessioniPrimary (citable) accession number: Q5UQ00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 52 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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