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Q5UQ00

- FPG_MIMIV

UniProt

Q5UQ00 - FPG_MIMIV

Protein

Probable formamidopyrimidine-DNA glycosylase

Gene

MIMI_L315

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNAPROSITE-ProRule annotation
    Active sitei3 – 31Proton donorPROSITE-ProRule annotation
    Active sitei58 – 581Proton donor; for beta-elimination activityPROSITE-ProRule annotation
    Active sitei277 – 2771Proton donor; for delta-elimination activityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. oxidized purine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-EC
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable formamidopyrimidine-DNA glycosylase (EC:3.2.2.23)
    Short name:
    Fapy-DNA glycosylase
    Alternative name(s):
    DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
    Short name:
    AP lyase
    Gene namesi
    Ordered Locus Names:MIMI_L315
    OrganismiAcanthamoeba polyphaga mimivirus (APMV)
    Taxonomic identifieri212035 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
    Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
    ProteomesiUP000001134: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by hostBy similarity
    Chaini2 – 287286Probable formamidopyrimidine-DNA glycosylasePRO_0000170892Add
    BLAST

    Structurei

    Secondary structure

    1
    287
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1815
    Beta strandi22 – 287
    Helixi32 – 354
    Helixi41 – 455
    Beta strandi49 – 5810
    Beta strandi61 – 688
    Beta strandi71 – 8111
    Turni83 – 853
    Beta strandi86 – 916
    Beta strandi96 – 1027
    Helixi103 – 1053
    Beta strandi107 – 1126
    Beta strandi118 – 1236
    Helixi125 – 1328
    Turni139 – 1413
    Helixi147 – 1526
    Helixi157 – 1626
    Beta strandi164 – 1674
    Helixi173 – 18210
    Helixi191 – 1933
    Helixi196 – 21520
    Beta strandi219 – 2213
    Helixi223 – 2253
    Helixi226 – 2294
    Beta strandi230 – 2323
    Beta strandi244 – 2474
    Beta strandi259 – 2613
    Beta strandi265 – 2684
    Helixi273 – 2753
    Beta strandi278 – 2814
    Turni283 – 2853

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3A42X-ray2.60A1-287[»]
    3A45X-ray2.30A/B1-287[»]
    3A46X-ray2.20A/B1-287[»]
    3VK7X-ray2.10A/B1-287[»]
    3VK8X-ray2.00A/B1-287[»]
    4NRWX-ray2.84A/B2-287[»]
    ProteinModelPortaliQ5UQ00.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5UQ00.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FPG family.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view]
    SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5UQ00-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEGPEVALT ADILEKYFKG KTLEYIDFIS GRYSKSEPEG YDDFIANLPL    50
    KVSNVDTKGK FLWFELFDPN DKSNKWYIWN TFGLTGMWSL FEAKYTRAVL 100
    SFDNELMAYF SDMRNFGTFK FSNSEKELKR KLNELGPDFL KNDDIDISKI 150
    KKYKQPIVAL LMDQKKIGSG LGNYLVAEIL YRAKIDPHKL GSNLTDQEIE 200
    NLWYWIKYET KLAYDSNHIG YMVNLENESS KIGRKNYHPN IHPTEKEFDF 250
    LVYRKKKDPN GNKVIADKII GSGKNKRTTY WAPAIQK 287
    Length:287
    Mass (Da):33,463
    Last modified:January 23, 2007 - v3
    Checksum:i745EE3385D9581AB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY653733 Genomic DNA. Translation: AAV50585.1.
    RefSeqiYP_003986818.1. NC_014649.1.

    Genome annotation databases

    GeneIDi9924932.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY653733 Genomic DNA. Translation: AAV50585.1 .
    RefSeqi YP_003986818.1. NC_014649.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3A42 X-ray 2.60 A 1-287 [» ]
    3A45 X-ray 2.30 A/B 1-287 [» ]
    3A46 X-ray 2.20 A/B 1-287 [» ]
    3VK7 X-ray 2.10 A/B 1-287 [» ]
    3VK8 X-ray 2.00 A/B 1-287 [» ]
    4NRW X-ray 2.84 A/B 2-287 [» ]
    ProteinModelPortali Q5UQ00.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 9924932.

    Miscellaneous databases

    EvolutionaryTracei Q5UQ00.

    Family and domain databases

    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view ]
    SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Rowbotham-Bradford.

    Entry informationi

    Entry nameiFPG_MIMIV
    AccessioniPrimary (citable) accession number: Q5UQ00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3