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Q5UQ00 (FPG_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable formamidopyrimidine-DNA glycosylase

Short name=Fapy-DNA glycosylase
EC=3.2.2.23
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Short name=AP lyase
EC=4.2.99.18
Gene names
Ordered Locus Names:MIMI_L315
OrganismAcanthamoeba polyphaga mimivirus (APMV) [Reference proteome]
Taxonomic identifier212035 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Sequence similarities

Belongs to the FPG family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host By similarity
Chain2 – 287286Probable formamidopyrimidine-DNA glycosylase
PRO_0000170892

Sites

Active site21Schiff-base intermediate with DNA By similarity
Active site31Proton donor By similarity
Active site581Proton donor; for beta-elimination activity By similarity
Active site2771Proton donor; for delta-elimination activity By similarity

Secondary structure

........................................................... 287
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5UQ00 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 745EE3385D9581AB

FASTA28733,463
        10         20         30         40         50         60 
MPEGPEVALT ADILEKYFKG KTLEYIDFIS GRYSKSEPEG YDDFIANLPL KVSNVDTKGK 

        70         80         90        100        110        120 
FLWFELFDPN DKSNKWYIWN TFGLTGMWSL FEAKYTRAVL SFDNELMAYF SDMRNFGTFK 

       130        140        150        160        170        180 
FSNSEKELKR KLNELGPDFL KNDDIDISKI KKYKQPIVAL LMDQKKIGSG LGNYLVAEIL 

       190        200        210        220        230        240 
YRAKIDPHKL GSNLTDQEIE NLWYWIKYET KLAYDSNHIG YMVNLENESS KIGRKNYHPN 

       250        260        270        280 
IHPTEKEFDF LVYRKKKDPN GNKVIADKII GSGKNKRTTY WAPAIQK 

« Hide

References

[1]"The 1.2-megabase genome sequence of Mimivirus."
Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M.
Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rowbotham-Bradford.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY653733 Genomic DNA. Translation: AAV50585.1.
RefSeqYP_003986818.1. NC_014649.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3A42X-ray2.60A1-287[»]
3A45X-ray2.30A/B1-287[»]
3A46X-ray2.20A/B1-287[»]
3VK7X-ray2.10A/B1-287[»]
3VK8X-ray2.00A/B1-287[»]
4NRWX-ray2.84A/B2-287[»]
ProteinModelPortalQ5UQ00.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9924932.

Family and domain databases

InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ5UQ00.

Entry information

Entry nameFPG_MIMIV
AccessionPrimary (citable) accession number: Q5UQ00
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 52 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references