Q5UQ00 (FPG_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable formamidopyrimidine-DNA glycosylase
Short name=Fapy-DNA glycosylase
EC=3.2.2.23

Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Short name=AP lyase
EC=4.2.99.18

Gene names

Ordered Locus Names:

MIMI_L315

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Reference proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Sequence similarities	Belongs to the FPG family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	DNA-binding
Molecular function	Glycosidase Hydrolase Lyase
Technical term	3D-structure Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: InterPro nucleotide-excision repair Inferred from electronic annotation. Source: InterPro
Molecular_function	damaged DNA binding Inferred from electronic annotation. Source: InterPro oxidized purine nucleobase lesion DNA N-glycosylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed; by host By similarity

Chain

2 – 287

286

Probable formamidopyrimidine-DNA glycosylase

PRO_0000170892

Sites

Active site

2

1

Schiff-base intermediate with DNA By similarity

Active site

3

1

Proton donor By similarity

Active site

58

1

Proton donor; for beta-elimination activity By similarity

Active site

277

1

Proton donor; for delta-elimination activity By similarity

Secondary structure

1

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287

Helix Strand Turn

Details...

Helix

4 – 18

15

Beta strand

22 – 28

7

Helix

32 – 35

4

Helix

41 – 45

5

Beta strand

49 – 58

10

Beta strand

61 – 68

8

Beta strand

71 – 81

11

Turn

83 – 85

3

Beta strand

86 – 91

6

Beta strand

96 – 102

7

Helix

103 – 105

3

Beta strand

107 – 112

6

Beta strand

118 – 123

6

Helix

125 – 132

8

Turn

139 – 141

3

Helix

147 – 152

6

Helix

157 – 162

6

Beta strand

164 – 167

4

Helix

173 – 182

10

Helix

191 – 193

3

Helix

196 – 215

20

Beta strand

219 – 221

3

Helix

223 – 225

3

Helix

226 – 229

4

Beta strand

244 – 247

4

Beta strand

259 – 261

3

Beta strand

265 – 268

4

Helix

273 – 275

3

Beta strand

278 – 281

4

Turn

283 – 285

3

Sequences

	Sequence		Length	Mass (Da)
	Q5UQ00 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 745EE3385D9581AB	FASTA	287	33,463
	10 20 30 40 50 60 MPEGPEVALT ADILEKYFKG KTLEYIDFIS GRYSKSEPEG YDDFIANLPL KVSNVDTKGK 70 80 90 100 110 120 FLWFELFDPN DKSNKWYIWN TFGLTGMWSL FEAKYTRAVL SFDNELMAYF SDMRNFGTFK 130 140 150 160 170 180 FSNSEKELKR KLNELGPDFL KNDDIDISKI KKYKQPIVAL LMDQKKIGSG LGNYLVAEIL 190 200 210 220 230 240 YRAKIDPHKL GSNLTDQEIE NLWYWIKYET KLAYDSNHIG YMVNLENESS KIGRKNYHPN 250 260 270 280 IHPTEKEFDF LVYRKKKDPN GNKVIADKII GSGKNKRTTY WAPAIQK

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY653733 Genomic DNA. Translation: AAV50585.1.

RefSeq

YP_003986818.1. NC_014649.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3A42	X-ray	2.60	A	1-287	[»]
3A45	X-ray	2.30	A/B	1-287	[»]
3A46	X-ray	2.20	A/B	1-287	[»]
3VK7	X-ray	2.10	A/B	1-287	[»]
3VK8	X-ray	2.00	A/B	1-287	[»]

ProteinModelPortal

Q5UQ00.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

9924932.

Family and domain databases

InterPro

IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view]

Pfam

PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]

SMART

SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]

SUPFAM

SSF81624. Form_DNAglyc_cat. 1 hit.
SSF46946. Ribosomal_H2TH. 1 hit.

PROSITE

PS51068. FPG_CAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q5UQ00.

Entry information

Entry name

FPG_MIMIV

Accession

Primary (citable) accession number: Q5UQ00

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	January 23, 2007
Last modified:	October 3, 2012
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program