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Q5UQ00

- FPG_MIMIV

UniProt

Q5UQ00 - FPG_MIMIV

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Protein

Probable formamidopyrimidine-DNA glycosylase

Gene

MIMI_L315

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNAPROSITE-ProRule annotation
Active sitei3 – 31Proton donorPROSITE-ProRule annotation
Active sitei58 – 581Proton donor; for beta-elimination activityPROSITE-ProRule annotation
Active sitei277 – 2771Proton donor; for delta-elimination activityPROSITE-ProRule annotation

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. oxidized purine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-EC
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable formamidopyrimidine-DNA glycosylase (EC:3.2.2.23)
Short name:
Fapy-DNA glycosylase
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Ordered Locus Names:MIMI_L315
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 287286Probable formamidopyrimidine-DNA glycosylasePRO_0000170892Add
BLAST

Structurei

Secondary structure

1
287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1815Combined sources
Beta strandi22 – 287Combined sources
Helixi32 – 354Combined sources
Helixi41 – 455Combined sources
Beta strandi49 – 5810Combined sources
Beta strandi61 – 688Combined sources
Beta strandi71 – 8111Combined sources
Turni83 – 853Combined sources
Beta strandi86 – 916Combined sources
Beta strandi96 – 1027Combined sources
Helixi103 – 1053Combined sources
Beta strandi107 – 1126Combined sources
Beta strandi118 – 1236Combined sources
Helixi125 – 1328Combined sources
Turni139 – 1413Combined sources
Helixi147 – 1526Combined sources
Helixi157 – 1626Combined sources
Beta strandi164 – 1674Combined sources
Helixi173 – 18210Combined sources
Helixi191 – 1933Combined sources
Helixi196 – 21520Combined sources
Beta strandi219 – 2213Combined sources
Helixi223 – 2253Combined sources
Helixi226 – 2294Combined sources
Beta strandi230 – 2323Combined sources
Beta strandi244 – 2474Combined sources
Beta strandi259 – 2613Combined sources
Beta strandi265 – 2684Combined sources
Helixi273 – 2753Combined sources
Beta strandi278 – 2814Combined sources
Turni283 – 2853Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A42X-ray2.60A1-287[»]
3A45X-ray2.30A/B1-287[»]
3A46X-ray2.20A/B1-287[»]
3VK7X-ray2.10A/B1-287[»]
3VK8X-ray2.00A/B1-287[»]
4NRWX-ray2.84A/B2-287[»]
ProteinModelPortaliQ5UQ00.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5UQ00.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5UQ00-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPEGPEVALT ADILEKYFKG KTLEYIDFIS GRYSKSEPEG YDDFIANLPL
60 70 80 90 100
KVSNVDTKGK FLWFELFDPN DKSNKWYIWN TFGLTGMWSL FEAKYTRAVL
110 120 130 140 150
SFDNELMAYF SDMRNFGTFK FSNSEKELKR KLNELGPDFL KNDDIDISKI
160 170 180 190 200
KKYKQPIVAL LMDQKKIGSG LGNYLVAEIL YRAKIDPHKL GSNLTDQEIE
210 220 230 240 250
NLWYWIKYET KLAYDSNHIG YMVNLENESS KIGRKNYHPN IHPTEKEFDF
260 270 280
LVYRKKKDPN GNKVIADKII GSGKNKRTTY WAPAIQK
Length:287
Mass (Da):33,463
Last modified:January 23, 2007 - v3
Checksum:i745EE3385D9581AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAV50585.1.
RefSeqiYP_003986818.1. NC_014649.1.

Genome annotation databases

GeneIDi9924932.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAV50585.1 .
RefSeqi YP_003986818.1. NC_014649.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3A42 X-ray 2.60 A 1-287 [» ]
3A45 X-ray 2.30 A/B 1-287 [» ]
3A46 X-ray 2.20 A/B 1-287 [» ]
3VK7 X-ray 2.10 A/B 1-287 [» ]
3VK8 X-ray 2.00 A/B 1-287 [» ]
4NRW X-ray 2.84 A/B 2-287 [» ]
ProteinModelPortali Q5UQ00.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9924932.

Miscellaneous databases

EvolutionaryTracei Q5UQ00.

Family and domain databases

InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.

Entry informationi

Entry nameiFPG_MIMIV
AccessioniPrimary (citable) accession number: Q5UQ00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 54 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3