Q5UP57 (P4H_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative prolyl 4-hydroxylase
Short name=4-PH
EC=1.14.11.2

Alternative name(s):
Procollagen-proline,2-oxoglutarate-4-dioxygenase

Gene names

Ordered Locus Names:

MIMI_L593

Organism

Acanthamoeba polyphaga mimivirus (APMV)

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in the 6 collagen-like proteins of Mimivirus Potential.
Catalytic activity	L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO2.
Cofactor	Iron. Ascorbate.
Subcellular location	Virion Ref.2.
Sequence similarities	Belongs to the P4HA family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Cellular component	Virion
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW procollagen-proline 4-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Putative prolyl 4-hydroxylase		PRO_0000206660
Regions
	Domain	128 – 238	111	Fe2OG dioxygenase

Sequences

	Sequence		Length	Mass (Da)
	Q5UP57 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: EF3D0F965EC41C2A	FASTA	242	27,901
	10 20 30 40 50 60 MKTVTIITII VVIIVVILII MVLSKSCVSH FRNVGSLNSR DVNLKDDFSY ANIDDPYNKP 70 80 90 100 110 120 FVLNNLINPT KCQEIMQFAN GKLFDSQVLS GTDKNIRNSQ QMWISKNNPM VKPIFENICR 130 140 150 160 170 180 QFNVPFDNAE DLQVVRYLPN QYYNEHHDSC CDSSKQCSEF IERGGQRILT VLIYLNNEFS 190 200 210 220 230 240 DGHTYFPNLN QKFKPKTGDA LVFYPLANNS NKCHPYSLHA GMPVTSGEKW IANLWFRERK FS

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.
[2]	"Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products." Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D. J. Virol. 80:11678-11685(2006) [PubMed: 16971431] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY653733 Genomic DNA. Translation: AAV50856.1.
RefSeq	YP_003987108.1. NC_014649.1.
3D structure databases
ProteinModelPortal	Q5UP57.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9925229.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. IPR006620. Pro_4_hyd_alph. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
SMART	SM00702. P4Hc. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P4H_MIMIV

Accession

Primary (citable) accession number: Q5UP57

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 7, 2004
Last modified:	September 21, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program