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Q5UEW0 (NCAP_I18A0) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name=Protein N
Gene names
Name:NP
OrganismInfluenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1)) [Complete proteome]
Taxonomic identifier88776 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.

Subunit structure

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.

Subcellular location

Virion Potential. Host nucleus By similarity.

Post-translational modification

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction By similarity.

Miscellaneous

South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.

Sequence similarities

Belongs to the influenza viruses nucleoprotein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Nucleoprotein
PRO_0000310567

Regions

Motif1 – 1818Unconventional nuclear localization signal By similarity
Motif198 – 21619Bipartite nuclear localization signal By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5UEW0 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 7DD6C8837A143F00

FASTA49856,304
        10         20         30         40         50         60 
MASQGTKRSY EQMETDGERQ NATEIRASVG RMIGGIGRFY IQMCTELKLS DYEGRLIQNS 

        70         80         90        100        110        120 
ITIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYRRI DGKWMRELIL YDKEEIRRIW 

       130        140        150        160        170        180 
RQANNGEDAT AGLTHMMIWH SNLNDATYQR TRALVRTGMD PRMCSLMQGS TLPRRSGAAG 

       190        200        210        220        230        240 
AAVKGVGTMV MELIRMIKRG INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQRAMMD 

       250        260        270        280        290        300 
QVRESRNPGN AEIEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEREGYSLVG 

       310        320        330        340        350        360 
IDPFRLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT RVVPRGKLST 

       370        380        390        400        410        420 
RGVQIASNEN METMDSSTLE LRSRYWAIRT RSGGNTNQQR ASAGQISVQP TFSVQRNLPF 

       430        440        450        460        470        480 
ERATIMAAFT GNTEGRTSDM RTEIIRMMES ARPEDVSFQG RGVFELSDEK ATSPIVPSFD 

       490 
MSNEGSYFFG DNAEEYDN 

« Hide

References

[1]"Novel origin of the 1918 pandemic influenza virus nucleoprotein gene."
Reid A.H., Fanning T.G., Janczewski T.A., Lourens R.M., Taubenberger J.K.
J. Virol. 78:12462-12470(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Initial genetic characterization of the 1918 'Spanish' influenza virus."
Taubenberger J.K., Reid A.H., Krafft A.E., Bijwaard K.E., Fanning T.G.
Science 275:1793-1796(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 166-186, OF 292-314.
Strain: A/South Carolina/1/18.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY744935 mRNA. Translation: AAV48837.1.
U94894 Genomic RNA. Translation: AAC57071.1.
U94895 Genomic RNA. Translation: AAC57072.1.

3D structure databases

ProteinModelPortalQ5UEW0.
SMRQ5UEW0. Positions 22-496.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002141. Flu_NP.
[Graphical view]
PfamPF00506. Flu_NP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNCAP_I18A0
AccessionPrimary (citable) accession number: Q5UEW0
Secondary accession number(s): O10422, O10423
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 7, 2004
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families