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Q5UEW0

- NCAP_I18A0

UniProt

Q5UEW0 - NCAP_I18A0

Protein

Nucleoprotein

Gene

NP

Organism
Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. viral penetration into host nucleus Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

    Keywords - Ligandi

    RNA-binding, Viral nucleoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoprotein
    Alternative name(s):
    Nucleocapsid protein
    Short name:
    Protein N
    Gene namesi
    Name:NP
    OrganismiInfluenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1))
    Taxonomic identifieri88776 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008430: Genome

    Subcellular locationi

    Virion Curated. Host nucleus By similarity

    GO - Cellular componenti

    1. helical viral capsid Source: UniProtKB-KW
    2. host cell nucleus Source: UniProtKB-SubCell
    3. ribonucleoprotein complex Source: UniProtKB-KW
    4. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Helical capsid protein, Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 498498NucleoproteinPRO_0000310567Add
    BLAST

    Post-translational modificationi

    Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction By similarity.By similarity

    Interactioni

    Subunit structurei

    Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5UEW0.
    SMRiQ5UEW0. Positions 22-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1 – 1818Unconventional nuclear localization signalBy similarityAdd
    BLAST
    Motifi198 – 21619Bipartite nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR002141. Flu_NP.
    [Graphical view]
    PfamiPF00506. Flu_NP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5UEW0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASQGTKRSY EQMETDGERQ NATEIRASVG RMIGGIGRFY IQMCTELKLS    50
    DYEGRLIQNS ITIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYRRI 100
    DGKWMRELIL YDKEEIRRIW RQANNGEDAT AGLTHMMIWH SNLNDATYQR 150
    TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG 200
    INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN 250
    AEIEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEREGYSLVG 300
    IDPFRLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT 350
    RVVPRGKLST RGVQIASNEN METMDSSTLE LRSRYWAIRT RSGGNTNQQR 400
    ASAGQISVQP TFSVQRNLPF ERATIMAAFT GNTEGRTSDM RTEIIRMMES 450
    ARPEDVSFQG RGVFELSDEK ATSPIVPSFD MSNEGSYFFG DNAEEYDN 498
    Length:498
    Mass (Da):56,304
    Last modified:December 7, 2004 - v1
    Checksum:i7DD6C8837A143F00
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY744935 mRNA. Translation: AAV48837.1.
    U94894 Genomic RNA. Translation: AAC57071.1.
    U94895 Genomic RNA. Translation: AAC57072.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY744935 mRNA. Translation: AAV48837.1 .
    U94894 Genomic RNA. Translation: AAC57071.1 .
    U94895 Genomic RNA. Translation: AAC57072.1 .

    3D structure databases

    ProteinModelPortali Q5UEW0.
    SMRi Q5UEW0. Positions 22-496.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002141. Flu_NP.
    [Graphical view ]
    Pfami PF00506. Flu_NP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel origin of the 1918 pandemic influenza virus nucleoprotein gene."
      Reid A.H., Fanning T.G., Janczewski T.A., Lourens R.M., Taubenberger J.K.
      J. Virol. 78:12462-12470(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Initial genetic characterization of the 1918 'Spanish' influenza virus."
      Taubenberger J.K., Reid A.H., Krafft A.E., Bijwaard K.E., Fanning T.G.
      Science 275:1793-1796(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 166-186, OF 292-314.
      Strain: A/South Carolina/1/18.

    Entry informationi

    Entry nameiNCAP_I18A0
    AccessioniPrimary (citable) accession number: Q5UEW0
    Secondary accession number(s): O10422, O10423
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3