Q5UEW0 (NCAP_I18A0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoprotein

Alternative name(s):
Nucleocapsid protein
Short name=Protein N

Gene names

Name:

NP

Organism

Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1)) [Complete proteome]

Taxonomic identifier

88776 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.
Subunit structure	Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.
Subcellular location	Virion Potential. Host nucleus By similarity.
Post-translational modification	Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction By similarity.
Miscellaneous	South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.
Sequence similarities	Belongs to the influenza viruses nucleoprotein family.

Ontologies

Keywords
Biological process	Host-virus interaction Initiation of viral infection Viral penetration into host nucleus
Cellular component	Host nucleus Virion
Ligand	RNA-binding Viral nucleoprotein
Molecular function	Ribonucleoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Nucleoprotein		PRO_0000310567
Regions
	Motif	1 – 18	18	Unconventional nuclear localization signal By similarity
	Motif	198 – 216	19	Bipartite nuclear localization signal By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5UEW0 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 7DD6C8837A143F00	FASTA	498	56,304
	10 20 30 40 50 60 MASQGTKRSY EQMETDGERQ NATEIRASVG RMIGGIGRFY IQMCTELKLS DYEGRLIQNS 70 80 90 100 110 120 ITIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYRRI DGKWMRELIL YDKEEIRRIW 130 140 150 160 170 180 RQANNGEDAT AGLTHMMIWH SNLNDATYQR TRALVRTGMD PRMCSLMQGS TLPRRSGAAG 190 200 210 220 230 240 AAVKGVGTMV MELIRMIKRG INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQRAMMD 250 260 270 280 290 300 QVRESRNPGN AEIEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEREGYSLVG 310 320 330 340 350 360 IDPFRLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT RVVPRGKLST 370 380 390 400 410 420 RGVQIASNEN METMDSSTLE LRSRYWAIRT RSGGNTNQQR ASAGQISVQP TFSVQRNLPF 430 440 450 460 470 480 ERATIMAAFT GNTEGRTSDM RTEIIRMMES ARPEDVSFQG RGVFELSDEK ATSPIVPSFD 490 MSNEGSYFFG DNAEEYDN

References

[1]	"Novel origin of the 1918 pandemic influenza virus nucleoprotein gene." Reid A.H., Fanning T.G., Janczewski T.A., Lourens R.M., Taubenberger J.K. J. Virol. 78:12462-12470(2004) [PubMed: 15507633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Initial genetic characterization of the 1918 'Spanish' influenza virus." Taubenberger J.K., Reid A.H., Krafft A.E., Bijwaard K.E., Fanning T.G. Science 275:1793-1796(1997) [PubMed: 9065404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 166-186, OF 292-314. Strain: A/South Carolina/1/18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY744935 mRNA. Translation: AAV48837.1. U94894 Genomic RNA. Translation: AAC57071.1. U94895 Genomic RNA. Translation: AAC57072.1.
3D structure databases
ProteinModelPortal	Q5UEW0.
SMR	Q5UEW0. Positions 22-496.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002141. Flu_NP. [Graphical view]
Pfam	PF00506. Flu_NP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCAP_I18A0

Accession

Primary (citable) accession number: Q5UEW0
Secondary accession number(s): O10422, O10423

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	December 7, 2004
Last modified:	May 3, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program