Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5UEA2

- Q5UEA2_9ENTO

UniProt

Q5UEA2 - Q5UEA2_9ENTO

Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Coxsackievirus B3
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    NTP + H2O = NDP + phosphate.SAAS annotation
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation
    Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei1768 – 17681Sulfate 2
    Binding sitei1820 – 18201Acetate; via amide nitrogenImported
    Binding sitei1897 – 18971Sulfate 1
    Binding sitei1897 – 18971Sulfate 4
    Binding sitei1911 – 19111Sulfate 3
    Binding sitei1959 – 19591Sulfate 4; via amide nitrogen
    Binding sitei1990 – 19901Sulfate 6
    Binding sitei2000 – 20001Sulfate 2
    Binding sitei2099 – 20991Sulfate 1

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. ion channel activity Source: UniProtKB-KW
    4. RNA binding Source: UniProtKB-KW
    5. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    6. RNA helicase activity Source: InterPro
    7. structural molecule activity Source: InterPro

    GO - Biological processi

    1. pore formation by virus in membrane of host cell Source: UniProtKB-KW
    2. protein oligomerization Source: UniProtKB-KW
    3. RNA-protein covalent cross-linking Source: InterPro
    4. transcription, DNA-templated Source: InterPro
    5. viral entry into host cell Source: UniProtKB-KW
    6. viral RNA genome replication Source: InterPro
    7. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    HelicaseSAAS annotation, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Thiol proteaseSAAS annotation, Transferase, Viral ion channelSAAS annotation

    Keywords - Biological processi

    Host-virus interaction, Ion transport, Transport, Viral attachment to host cellSAAS annotation, Viral RNA replicationSAAS annotation, Virus entry into host cell

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-bindingSAAS annotation

    Protein family/group databases

    MEROPSiN08.001.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    PolyproteinImported
    OrganismiCoxsackievirus B3Imported
    Taxonomic identifieri12072 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus B

    Subcellular locationi

    Host cytoplasm SAAS annotation
    Host cytoplasmic vesicle membrane SAAS annotation; Peripheral membrane protein SAAS annotation; Cytoplasmic side SAAS annotation

    GO - Cellular componenti

    1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    2. integral to membrane of host cell Source: UniProtKB-KW
    3. membrane Source: UniProtKB-KW
    4. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid proteinSAAS annotation, Host cytoplasmSAAS annotation, Host cytoplasmic vesicleSAAS annotation, Host membraneSAAS annotation, Membrane, Virion

    PTM / Processingi

    Keywords - PTMi

    PhosphoproteinSAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DDKX-ray2.25A1724-2185[»]
    3ZYDX-ray1.70A1541-1723[»]
    3ZYEX-ray1.85A1541-1723[»]
    3ZZ3X-ray1.89A/B1541-1723[»]
    3ZZ4X-ray2.10A/B1541-1723[»]
    3ZZ5X-ray2.20A1541-1723[»]
    3ZZ6X-ray2.05A1541-1723[»]
    3ZZ7X-ray1.80A1541-1723[»]
    3ZZ8X-ray1.85A1541-1723[»]
    3ZZ9X-ray1.90A1541-1723[»]
    3ZZAX-ray1.80A1541-1723[»]
    3ZZBX-ray2.10A1541-1723[»]
    3ZZCX-ray2.10A1541-1723[»]
    3ZZDX-ray2.10A1541-1723[»]
    4K4XX-ray2.37A/E/I/M1724-2185[»]
    4K4YX-ray2.72A/E/I/M1724-2185[»]
    4K4ZX-ray2.17A/E/I/M1724-2185[»]
    ProteinModelPortaliQ5UEA2.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5UEA2.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1950 – 19523Sulfate 5 binding

    Sequence similaritiesi

    Contains RdRp catalytic domain.SAAS annotation
    Contains SF3 helicase domain.SAAS annotation
    Contains SFhelicase domain.SAAS annotation

    Family and domain databases

    Gene3Di2.60.120.20. 3 hits.
    4.10.80.10. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR003138. Pico_P1A.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF02226. Pico_P1A. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 3 hits.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    ProDomiPD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5UEA2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGAQVSTQKT GAHETGLNAS GNSIIHYTNV NYYKDAASNS ANRQDFTQDP     50
    GKFTEPVKDI MIKSLPALNS PTVEECGYSD RARSITLGNS TITTQECANV 100
    VVGYGVWPDY LKDSEATAED QPTQPDVATC RFYTLDSVQW QKTSPGWWWK 150
    LPDALSNLGL FGQNMQYHYL GRTGYTVHVQ CNASKFHQGC LLVVCVPEAE 200
    MGCATLDNTP SSAELLGGDS AKEFADKPVA SGSNKLVQRV VYNAGMGVGV 250
    GNLTIFPHQW INLRTNNSAT IVMPYTNSVP MDNMFRHNNV TLMVIPFVPL 300
    DYCPGSTTYV PITVTIAPMC AEYNGLRLAG HQGLPTMNTP GSCQFLTSDD 350
    FQSPSAMPQY DVTPEMRIPG EVKNLMEIAE VDSVVPVQNV GEKVNSMEAY 400
    QIPVRSNEGS GTQVFGFPLQ PGYSSVFSRT LLGEILNYYT HWSGSIKLTF 450
    MFCGSAMATG KFLLAYSPPG AGAPTKRVDA MLGTHVVWDV GLQSSCVLCI 500
    PWISQTHYRY VASDEYTAGG FITCWYQTNI VVPADAQSSC YIMCFVSACN 550
    DFSVRLLKDT PFISQENFFQ GPVEDAITAA IGRVADTVGT GPTNSEAIPA 600
    LTAAETGHTS QVVPGDTMQT RHVKNYHSRS ESTIENFLCR SACVYFTEYE 650
    NSGAKRYAEW VLTPRQAAQL RRKLEFFTYV RFDLELTFVI TSTQQPSTTQ 700
    NQDAQILTHQ IMYVPPGGPV PDKVDSYVWQ TSTNPSVFWT EGNAPPRMSI 750
    PFLSIGNAYS NFYDGWSEFS RNGVYGINTL NNMGTLYARH VNAGSTGPIK 800
    STIRIYFKPK HVKAWIPRPP RLCQYEKAKN VNFRPSGVTT TRQSITTMTN 850
    TGAFGQQSGA AYVGNYRVVN RHLATSADWQ NCVWESYNRD LLVSTTTAHG 900
    CDIIARCQCT TGVYFCASKN KHYPISFEGP GLVEVQESEY YPRRYQSHVL 950
    LAAGFSEPGD CGGILRCEHG VIGIVTMGGE GVVGFADIRD LLWLEDDAME 1000
    QGVKDYVEQL GNAFGSGFTN RICEQVNLLK ESLVGQDSIL EKSLKALVKI 1050
    ISALVIVVRN HDDLITVTAT LALIGCTSSP WRWLKQKVSQ YYGIPLAERQ 1100
    NNSWLKKFTE MTNACKGMEW IAVKIQKFIE WLKVKILPEV REKHEFLNRL 1150
    KQLPLLESQI ATIEQSAPSQ SDQEQLFSNV QYFAHYCRKY APLYAAEAKR 1200
    VFSLEKKMSN YIQFKSKCRI EPVCLLLHGS PGAGKSVATN LIGRSLAEKL 1250
    NSSVYSLPPD PDHFDGYKQQ AVVIMDDLCQ NPDGKDVSLF CQMVSSVDFV 1300
    PPMAALEEKG ILFTSPFVLA STNAGSINAP TVSDSRALAR RFHFDMNIEV 1350
    ISMYSQNGKI NMPMSVKTCD DECCPVNFKK CCPLVCGKAI QFIDRRTQVR 1400
    YSLDMLVTEM FREYNHRHSV GTTLEALFQG PPVYREIKIS VAPETPPPPA 1450
    IADLLKSVDS EAVREYCKEK GWLVPEINST LQIEKHVSRA FICLQALTTF 1500
    VSVAGIIYII YKLFAGFQGA YTGVPNQKPR VPTLRQAKVQ GPAFEFAVAM 1550
    MKRNSSTVKT EYGEFTMLGI YDRWAVLPRH AKPGPTILMN DQEVGVLDAK 1600
    ELVDKDGTNL ELTLLKLNRN EKFRDIRGFL AKEEVEVNEA VLAINTSKFP 1650
    NMYIPVGQVT EYGFLNLGGT PTKRMLMYNF PTRAGQCGGV LMSTGKVLGI 1700
    HVGGNGHQGF SAALLKHYFN DEQGEIEFIE SSKDAGFPVI NTPSKTKLEP 1750
    SVFHQVFEGN KEPAVLRSGD PRLKANFEEA IFSKYIGNVN THVDEYMLEA 1800
    VDHYAGQLAT LDISTEPMKL EDAVYGTEGL EALDLTTSAG YPYVALGIKK 1850
    RDILSKKTKD LTKLKECMDK YGLNLPMVTY VKDELRSIEK VAKGKSRLIE 1900
    ASSLNDSVAM RQTFGNLYKT FHLNPGVVTG SAVGCDPDLF WSKIPVMLDG 1950
    HLIAFDYSGY DASLSPVWFA CLKMLLEKLG YTHKETNYID YLCNSHHLYR 2000
    DKHYFVRGGM PSGCSGTSIF NSMINNIIIR TLMLKVYKGI DLDQFRMIAY 2050
    GDDVIASYPW PIDASLLAEA GKGYGLIMTP ADKGECFNEV TWTNVTFLKR 2100
    YFRADEQYPF LVHPVMPMKD IHESIRWTKD PKNTQDHVRS LCLLAWHNGE 2150
    HEYEEFIRKI RSVPVGRCLT LPAFSTLRRK WLDSF 2185
    Length:2,185
    Mass (Da):243,368
    Last modified:December 7, 2004 - v1
    Checksum:i86068AAD2A4DF167
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY752945 Genomic RNA. Translation: AAV34212.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY752945 Genomic RNA. Translation: AAV34212.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3DDK X-ray 2.25 A 1724-2185 [» ]
    3ZYD X-ray 1.70 A 1541-1723 [» ]
    3ZYE X-ray 1.85 A 1541-1723 [» ]
    3ZZ3 X-ray 1.89 A/B 1541-1723 [» ]
    3ZZ4 X-ray 2.10 A/B 1541-1723 [» ]
    3ZZ5 X-ray 2.20 A 1541-1723 [» ]
    3ZZ6 X-ray 2.05 A 1541-1723 [» ]
    3ZZ7 X-ray 1.80 A 1541-1723 [» ]
    3ZZ8 X-ray 1.85 A 1541-1723 [» ]
    3ZZ9 X-ray 1.90 A 1541-1723 [» ]
    3ZZA X-ray 1.80 A 1541-1723 [» ]
    3ZZB X-ray 2.10 A 1541-1723 [» ]
    3ZZC X-ray 2.10 A 1541-1723 [» ]
    3ZZD X-ray 2.10 A 1541-1723 [» ]
    4K4X X-ray 2.37 A/E/I/M 1724-2185 [» ]
    4K4Y X-ray 2.72 A/E/I/M 1724-2185 [» ]
    4K4Z X-ray 2.17 A/E/I/M 1724-2185 [» ]
    ProteinModelPortali Q5UEA2.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi N08.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q5UEA2.

    Family and domain databases

    Gene3Di 2.60.120.20. 3 hits.
    4.10.80.10. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR003138. Pico_P1A.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF02226. Pico_P1A. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 3 hits.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    ProDomi PD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An infectious cDNA copy of the genome of a non-cardiovirulent coxsackievirus B3 strain: its complete sequence analysis and comparison to the genomes of cardiovirulent coxsackieviruses."
      Chapman N.M., Tu Z., Tracy S., Gauntt C.J.
      Arch. Virol. 135:115-130(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 0Imported.
    2. Chapman N.M., Tu Z., Tracy S., Gauntt C.J.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 0Imported.
    3. "Crystal structure of coxsackievirus B3 3Dpol highlights the functional importance of residue 5 in picornavirus polymerases."
      Campagnola G., Weygandt M., Scoggin K., Peersen O.
      J. Virol. 82:9458-9464(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1724-2185.
    4. "Peptidic Ab-Nonsaturated Ethyl Esters as Inhibitors of the 3C Protease of Coxsackie Virus B3: Crystal Structures, Antiviral Activities, and Resistance Mutations."
      Tan J., Anand K., Mesters J.R., Hilgenfeld R.
      Submitted (AUG-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 1541-1723.
    5. "Peptidic Alpha, Beta-Unsaturated Ethyl Esters as Inhibitors of the 3C Protease of Coxsackie Virus B3: Crystal Structures, Antiviral Activities, and Resistance Mutations."
      Tan J., Anand K., Mesters J.R., Hilgenfeld R.
      Submitted (AUG-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 1541-1723.
    6. "Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts."
      Gong P., Kortus M.G., Nix J.C., Davis R.E., Peersen O.B.
      PLoS ONE 8:e60272-e60272(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.17 ANGSTROMS) OF 1724-2185.

    Entry informationi

    Entry nameiQ5UEA2_9ENTO
    AccessioniPrimary (citable) accession number: Q5UEA2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: December 7, 2004
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3