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Q5UEA2

- Q5UEA2_9ENTO

UniProt

Q5UEA2 - Q5UEA2_9ENTO

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Protein
Submitted name: Polyprotein
Gene
N/A
Organism
Human coxsackievirus B3
Status
Unreviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

NTP + H2O = NDP + phosphate.SAAS annotations
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotations
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.SAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1768 – 17681Sulfate 2
Binding sitei1820 – 18201Acetate; via amide nitrogenImported
Binding sitei1897 – 18971Sulfate 1
Binding sitei1897 – 18971Sulfate 4
Binding sitei1911 – 19111Sulfate 3
Binding sitei1959 – 19591Sulfate 4; via amide nitrogen
Binding sitei1990 – 19901Sulfate 6
Binding sitei2000 – 20001Sulfate 2
Binding sitei2099 – 20991Sulfate 1

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA helicase activity Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. cysteine-type endopeptidase activity Source: InterPro
  6. ion channel activity Source: UniProtKB-KW
  7. structural molecule activity Source: InterPro

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: InterPro
  2. pore formation by virus in membrane of host cell Source: UniProtKB-KW
  3. protein oligomerization Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
  5. viral RNA genome replication Source: InterPro
  6. viral entry into host cell Source: UniProtKB-KW
  7. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HelicaseSAAS annotations, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotations, Thiol proteaseSAAS annotations, Transferase, Viral ion channelSAAS annotations

Keywords - Biological processi

Host-virus interaction, Ion transport, Transport, Viral attachment to host cellSAAS annotations, Viral RNA replicationSAAS annotations, Virus entry into host cell

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-bindingSAAS annotations

Protein family/group databases

MEROPSiN08.001.

Names & Taxonomyi

Protein namesi
Submitted name:
PolyproteinImported
OrganismiHuman coxsackievirus B3Imported
Taxonomic identifieri12072 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus B

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  2. integral to membrane of host cell Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
  4. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid proteinSAAS annotations, Host cytoplasmSAAS annotations, Host cytoplasmic vesicleSAAS annotations, Host membraneSAAS annotations, Membrane, Virion

PTM / Processingi

Keywords - PTMi

PhosphoproteinSAAS annotations

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DDKX-ray2.25A1724-2185[»]
3ZYDX-ray1.70A1541-1723[»]
3ZYEX-ray1.85A1541-1723[»]
3ZZ3X-ray1.89A/B1541-1723[»]
3ZZ4X-ray2.10A/B1541-1723[»]
3ZZ5X-ray2.20A1541-1723[»]
3ZZ6X-ray2.05A1541-1723[»]
3ZZ7X-ray1.80A1541-1723[»]
3ZZ8X-ray1.85A1541-1723[»]
3ZZ9X-ray1.90A1541-1723[»]
3ZZAX-ray1.80A1541-1723[»]
3ZZBX-ray2.10A1541-1723[»]
3ZZCX-ray2.10A1541-1723[»]
3ZZDX-ray2.10A1541-1723[»]
4K4XX-ray2.37A/E/I/M1724-2185[»]
4K4YX-ray2.72A/E/I/M1724-2185[»]
4K4ZX-ray2.17A/E/I/M1724-2185[»]
ProteinModelPortaliQ5UEA2.

Miscellaneous databases

EvolutionaryTraceiQ5UEA2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1950 – 19523Sulfate 5 binding

Sequence similaritiesi

Contains RdRp catalytic domain.SAAS annotations
Contains SF3 helicase domain.SAAS annotations
Contains SFhelicase domain.SAAS annotations

Family and domain databases

Gene3Di2.60.120.20. 3 hits.
4.10.80.10. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
ProDomiPD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5UEA2-1 [UniParc]FASTAAdd to Basket

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MGAQVSTQKT GAHETGLNAS GNSIIHYTNV NYYKDAASNS ANRQDFTQDP     50
GKFTEPVKDI MIKSLPALNS PTVEECGYSD RARSITLGNS TITTQECANV 100
VVGYGVWPDY LKDSEATAED QPTQPDVATC RFYTLDSVQW QKTSPGWWWK 150
LPDALSNLGL FGQNMQYHYL GRTGYTVHVQ CNASKFHQGC LLVVCVPEAE 200
MGCATLDNTP SSAELLGGDS AKEFADKPVA SGSNKLVQRV VYNAGMGVGV 250
GNLTIFPHQW INLRTNNSAT IVMPYTNSVP MDNMFRHNNV TLMVIPFVPL 300
DYCPGSTTYV PITVTIAPMC AEYNGLRLAG HQGLPTMNTP GSCQFLTSDD 350
FQSPSAMPQY DVTPEMRIPG EVKNLMEIAE VDSVVPVQNV GEKVNSMEAY 400
QIPVRSNEGS GTQVFGFPLQ PGYSSVFSRT LLGEILNYYT HWSGSIKLTF 450
MFCGSAMATG KFLLAYSPPG AGAPTKRVDA MLGTHVVWDV GLQSSCVLCI 500
PWISQTHYRY VASDEYTAGG FITCWYQTNI VVPADAQSSC YIMCFVSACN 550
DFSVRLLKDT PFISQENFFQ GPVEDAITAA IGRVADTVGT GPTNSEAIPA 600
LTAAETGHTS QVVPGDTMQT RHVKNYHSRS ESTIENFLCR SACVYFTEYE 650
NSGAKRYAEW VLTPRQAAQL RRKLEFFTYV RFDLELTFVI TSTQQPSTTQ 700
NQDAQILTHQ IMYVPPGGPV PDKVDSYVWQ TSTNPSVFWT EGNAPPRMSI 750
PFLSIGNAYS NFYDGWSEFS RNGVYGINTL NNMGTLYARH VNAGSTGPIK 800
STIRIYFKPK HVKAWIPRPP RLCQYEKAKN VNFRPSGVTT TRQSITTMTN 850
TGAFGQQSGA AYVGNYRVVN RHLATSADWQ NCVWESYNRD LLVSTTTAHG 900
CDIIARCQCT TGVYFCASKN KHYPISFEGP GLVEVQESEY YPRRYQSHVL 950
LAAGFSEPGD CGGILRCEHG VIGIVTMGGE GVVGFADIRD LLWLEDDAME 1000
QGVKDYVEQL GNAFGSGFTN RICEQVNLLK ESLVGQDSIL EKSLKALVKI 1050
ISALVIVVRN HDDLITVTAT LALIGCTSSP WRWLKQKVSQ YYGIPLAERQ 1100
NNSWLKKFTE MTNACKGMEW IAVKIQKFIE WLKVKILPEV REKHEFLNRL 1150
KQLPLLESQI ATIEQSAPSQ SDQEQLFSNV QYFAHYCRKY APLYAAEAKR 1200
VFSLEKKMSN YIQFKSKCRI EPVCLLLHGS PGAGKSVATN LIGRSLAEKL 1250
NSSVYSLPPD PDHFDGYKQQ AVVIMDDLCQ NPDGKDVSLF CQMVSSVDFV 1300
PPMAALEEKG ILFTSPFVLA STNAGSINAP TVSDSRALAR RFHFDMNIEV 1350
ISMYSQNGKI NMPMSVKTCD DECCPVNFKK CCPLVCGKAI QFIDRRTQVR 1400
YSLDMLVTEM FREYNHRHSV GTTLEALFQG PPVYREIKIS VAPETPPPPA 1450
IADLLKSVDS EAVREYCKEK GWLVPEINST LQIEKHVSRA FICLQALTTF 1500
VSVAGIIYII YKLFAGFQGA YTGVPNQKPR VPTLRQAKVQ GPAFEFAVAM 1550
MKRNSSTVKT EYGEFTMLGI YDRWAVLPRH AKPGPTILMN DQEVGVLDAK 1600
ELVDKDGTNL ELTLLKLNRN EKFRDIRGFL AKEEVEVNEA VLAINTSKFP 1650
NMYIPVGQVT EYGFLNLGGT PTKRMLMYNF PTRAGQCGGV LMSTGKVLGI 1700
HVGGNGHQGF SAALLKHYFN DEQGEIEFIE SSKDAGFPVI NTPSKTKLEP 1750
SVFHQVFEGN KEPAVLRSGD PRLKANFEEA IFSKYIGNVN THVDEYMLEA 1800
VDHYAGQLAT LDISTEPMKL EDAVYGTEGL EALDLTTSAG YPYVALGIKK 1850
RDILSKKTKD LTKLKECMDK YGLNLPMVTY VKDELRSIEK VAKGKSRLIE 1900
ASSLNDSVAM RQTFGNLYKT FHLNPGVVTG SAVGCDPDLF WSKIPVMLDG 1950
HLIAFDYSGY DASLSPVWFA CLKMLLEKLG YTHKETNYID YLCNSHHLYR 2000
DKHYFVRGGM PSGCSGTSIF NSMINNIIIR TLMLKVYKGI DLDQFRMIAY 2050
GDDVIASYPW PIDASLLAEA GKGYGLIMTP ADKGECFNEV TWTNVTFLKR 2100
YFRADEQYPF LVHPVMPMKD IHESIRWTKD PKNTQDHVRS LCLLAWHNGE 2150
HEYEEFIRKI RSVPVGRCLT LPAFSTLRRK WLDSF 2185
Length:2,185
Mass (Da):243,368
Last modified:December 7, 2004 - v1
Checksum:i86068AAD2A4DF167
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY752945 Genomic RNA. Translation: AAV34212.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY752945 Genomic RNA. Translation: AAV34212.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3DDK X-ray 2.25 A 1724-2185 [» ]
3ZYD X-ray 1.70 A 1541-1723 [» ]
3ZYE X-ray 1.85 A 1541-1723 [» ]
3ZZ3 X-ray 1.89 A/B 1541-1723 [» ]
3ZZ4 X-ray 2.10 A/B 1541-1723 [» ]
3ZZ5 X-ray 2.20 A 1541-1723 [» ]
3ZZ6 X-ray 2.05 A 1541-1723 [» ]
3ZZ7 X-ray 1.80 A 1541-1723 [» ]
3ZZ8 X-ray 1.85 A 1541-1723 [» ]
3ZZ9 X-ray 1.90 A 1541-1723 [» ]
3ZZA X-ray 1.80 A 1541-1723 [» ]
3ZZB X-ray 2.10 A 1541-1723 [» ]
3ZZC X-ray 2.10 A 1541-1723 [» ]
3ZZD X-ray 2.10 A 1541-1723 [» ]
4K4X X-ray 2.37 A/E/I/M 1724-2185 [» ]
4K4Y X-ray 2.72 A/E/I/M 1724-2185 [» ]
4K4Z X-ray 2.17 A/E/I/M 1724-2185 [» ]
ProteinModelPortali Q5UEA2.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi N08.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q5UEA2.

Family and domain databases

Gene3Di 2.60.120.20. 3 hits.
4.10.80.10. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
ProDomi PD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "An infectious cDNA copy of the genome of a non-cardiovirulent coxsackievirus B3 strain: its complete sequence analysis and comparison to the genomes of cardiovirulent coxsackieviruses."
    Chapman N.M., Tu Z., Tracy S., Gauntt C.J.
    Arch. Virol. 135:115-130(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 0Imported.
  2. Chapman N.M., Tu Z., Tracy S., Gauntt C.J.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 0Imported.
  3. "Crystal structure of coxsackievirus B3 3Dpol highlights the functional importance of residue 5 in picornavirus polymerases."
    Campagnola G., Weygandt M., Scoggin K., Peersen O.
    J. Virol. 82:9458-9464(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1724-2185.
  4. "Peptidic Ab-Nonsaturated Ethyl Esters as Inhibitors of the 3C Protease of Coxsackie Virus B3: Crystal Structures, Antiviral Activities, and Resistance Mutations."
    Tan J., Anand K., Mesters J.R., Hilgenfeld R.
    Submitted (AUG-2011) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 1541-1723.
  5. "Peptidic Alpha, Beta-Unsaturated Ethyl Esters as Inhibitors of the 3C Protease of Coxsackie Virus B3: Crystal Structures, Antiviral Activities, and Resistance Mutations."
    Tan J., Anand K., Mesters J.R., Hilgenfeld R.
    Submitted (AUG-2011) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 1541-1723.
  6. "Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts."
    Gong P., Kortus M.G., Nix J.C., Davis R.E., Peersen O.B.
    PLoS ONE 8:e60272-e60272(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.17 ANGSTROMS) OF 1724-2185.

Entry informationi

Entry nameiQ5UEA2_9ENTO
AccessioniPrimary (citable) accession number: Q5UEA2
Entry historyi
Integrated into UniProtKB/TrEMBL: December 7, 2004
Last sequence update: December 7, 2004
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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