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Protein

Glucosamine 6-phosphate N-acetyltransferase 1

Gene

GNA1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acetyltransferase involved in de novo biosynthesis of UDP-N-acetylglucosamine (UDP-GlcNAc) in roots and is required for maintaining normal root cell shape. UDP-GlcNAc is an essential metabolite that serves as an initial sugar donor for N-glycan synthesis and thus plays an important role in protein and lipid glycosylation.1 Publication

Catalytic activityi

Acetyl-CoA + D-glucosamine 6-phosphate = CoA + N-acetyl-D-glucosamine 6-phosphate.1 Publication

Kineticsi

  1. KM=180 µM for acetyl-coenzyme A1 Publication
  2. KM=145 µM for glucosamine-6-phosphate1 Publication

    Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I).
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Probable glucosamine 6-phosphate N-acetyltransferase 2 (Os02g0717700), Glucosamine 6-phosphate N-acetyltransferase 1 (GNA1)
    2. Phosphoacetylglucosamine mutase (Os07g0195400)
    This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei44SubstrateBy similarity1

    GO - Molecular functioni

    • glucosamine 6-phosphate N-acetyltransferase activity Source: UniProtKB

    GO - Biological processi

    • N-acetylglucosamine biosynthetic process Source: UniProtKB
    • root development Source: UniProtKB
    • UDP-N-acetylglucosamine biosynthetic process Source: GO_Central

    Keywordsi

    Molecular functionAcyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiR-OSA-446210 Synthesis of UDP-N-acetyl-glucosamine
    UniPathwayiUPA00113; UER00529

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosamine 6-phosphate N-acetyltransferase 1 (EC:2.3.1.41 Publication)
    Alternative name(s):
    Glucose-6-phosphate acetyltransferase 1
    Short name:
    OsGNA1
    Phosphoglucosamine acetylase 1
    Phosphoglucosamine transacetylase 1
    Gene namesi
    Name:GNA1
    Ordered Locus Names:Os09g0488000, LOC_Os09g31310
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
    Proteomesi
    • UP000059680 Componenti: Chromosome 9

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Short roots, disruption of microtubules and shrinkage of cells in the root elongation zone.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004210381 – 165Glucosamine 6-phosphate N-acetyltransferase 1Add BLAST165

    Proteomic databases

    PaxDbiQ5U9F2

    Expressioni

    Tissue specificityi

    Highly expressed in the root elongation zone and at lower levels in leaves and grains.1 Publication

    Gene expression databases

    ExpressionAtlasiQ5U9F2 differential
    GenevisibleiQ5U9F2 OS

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi39947.LOC_Os09g31310.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ5U9F2
    SMRiQ5U9F2
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini22 – 165N-acetyltransferasePROSITE-ProRule annotationAdd BLAST144

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni92 – 95Substrate bindingBy similarity4
    Regioni104 – 106Substrate bindingBy similarity3
    Regioni114 – 119Acetyl-CoA bindingBy similarity6
    Regioni135 – 136Substrate bindingBy similarity2
    Regioni149 – 151Acetyl-CoA bindingBy similarity3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG3396 Eukaryota
    COG0454 LUCA
    InParanoidiQ5U9F2
    KOiK00621
    OMAiGRGCYKV
    OrthoDBiEOG09360S2X

    Family and domain databases

    InterProiView protein in InterPro
    IPR016181 Acyl_CoA_acyltransferase
    IPR000182 GNAT_dom
    PfamiView protein in Pfam
    PF00583 Acetyltransf_1, 1 hit
    SUPFAMiSSF55729 SSF55729, 1 hit
    PROSITEiView protein in PROSITE
    PS51186 GNAT, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q5U9F2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEQPLPTAAA EAAAAGGDGE AYRIRPLELA DISRGFLGLL NQLSPSPPLT
    60 70 80 90 100
    EEAFRARFEE LAALGADHLV LVAEDAATGR LAAAGAVLVE RKFIRRCGRV
    110 120 130 140 150
    GHVEDVVVDA AARGRGLGER VVRRLVEHAR GRGCYKVIIN CTPELTGFYA
    160
    KCGFVEKNVQ MGLYF
    Length:165
    Mass (Da):17,720
    Last modified:December 7, 2004 - v1
    Checksum:i3EB05150B80CDE95
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY772189 mRNA Translation: AAV40998.1
    AC108758 Genomic DNA No translation available.
    AP008215 Genomic DNA Translation: BAF25444.1
    AP014965 Genomic DNA Translation: BAT08714.1
    AK063214 mRNA Translation: BAG88597.1
    RefSeqiXP_015610795.1, XM_015755309.1
    UniGeneiOs.88036

    Genome annotation databases

    EnsemblPlantsiOs09t0488000-01; Os09t0488000-01; Os09g0488000
    GeneIDi4347432
    GrameneiOs09t0488000-01; Os09t0488000-01; Os09g0488000
    KEGGiosa:4347432

    Similar proteinsi

    Entry informationi

    Entry nameiGNA1_ORYSJ
    AccessioniPrimary (citable) accession number: Q5U9F2
    Secondary accession number(s): A0A0N7KR10
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2013
    Last sequence update: December 7, 2004
    Last modified: May 23, 2018
    This is version 86 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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