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Protein

Glucosamine 6-phosphate N-acetyltransferase 1

Gene

GNA1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acetyltransferase involved in de novo biosynthesis of UDP-N-acetylglucosamine (UDP-GlcNAc) in roots and is required for maintaining normal root cell shape. UDP-GlcNAc is an essential metabolite that serves as an initial sugar donor for N-glycan synthesis and thus plays an important role in protein and lipid glycosylation.1 Publication

Catalytic activityi

Acetyl-CoA + D-glucosamine 6-phosphate = CoA + N-acetyl-D-glucosamine 6-phosphate.1 Publication

Kineticsi

  1. KM=180 µM for acetyl-coenzyme A1 Publication
  2. KM=145 µM for glucosamine-6-phosphate1 Publication

    Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I).
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Probable glucosamine 6-phosphate N-acetyltransferase 2 (Os02g0717700), Glucosamine 6-phosphate N-acetyltransferase 1 (GNA1)
    2. Phosphoacetylglucosamine mutase (Os07g0195400)
    This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei44 – 441SubstrateBy similarity

    GO - Molecular functioni

    • glucosamine 6-phosphate N-acetyltransferase activity Source: UniProtKB

    GO - Biological processi

    • N-acetylglucosamine biosynthetic process Source: UniProtKB
    • root development Source: UniProtKB
    • UDP-N-acetylglucosamine biosynthetic process Source: GO_Central
    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiR-OSA-446210. Synthesis of UDP-N-acetyl-glucosamine.
    UniPathwayiUPA00113; UER00529.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosamine 6-phosphate N-acetyltransferase 1 (EC:2.3.1.41 Publication)
    Alternative name(s):
    Glucose-6-phosphate acetyltransferase 1
    Short name:
    OsGNA1
    Phosphoglucosamine acetylase 1
    Phosphoglucosamine transacetylase 1
    Gene namesi
    Name:GNA1
    Ordered Locus Names:Os09g0488000, LOC_Os09g31310
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
    Proteomesi
    • UP000059680 Componenti: Chromosome 9, cultivar: Nipponbare

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Short roots, disruption of microtubules and shrinkage of cells in the root elongation zone.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165Glucosamine 6-phosphate N-acetyltransferase 1PRO_0000421038Add
    BLAST

    Proteomic databases

    PaxDbiQ5U9F2.
    PRIDEiQ5U9F2.

    Expressioni

    Tissue specificityi

    Highly expressed in the root elongation zone and at lower levels in leaves and grains.1 Publication

    Gene expression databases

    GenevisibleiQ5U9F2. OS.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi39947.LOC_Os09g31310.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5U9F2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 165144N-acetyltransferasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni92 – 954Substrate bindingBy similarity
    Regioni104 – 1063Substrate bindingBy similarity
    Regioni114 – 1196Acetyl-CoA bindingBy similarity
    Regioni135 – 1362Substrate bindingBy similarity
    Regioni149 – 1513Acetyl-CoA bindingBy similarity

    Sequence similaritiesi

    Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiKOG3396. Eukaryota.
    COG0454. LUCA.
    InParanoidiQ5U9F2.
    KOiK00621.
    OMAiKFIHNCA.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view]
    PfamiPF00583. Acetyltransf_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5U9F2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEQPLPTAAA EAAAAGGDGE AYRIRPLELA DISRGFLGLL NQLSPSPPLT
    60 70 80 90 100
    EEAFRARFEE LAALGADHLV LVAEDAATGR LAAAGAVLVE RKFIRRCGRV
    110 120 130 140 150
    GHVEDVVVDA AARGRGLGER VVRRLVEHAR GRGCYKVIIN CTPELTGFYA
    160
    KCGFVEKNVQ MGLYF
    Length:165
    Mass (Da):17,720
    Last modified:December 7, 2004 - v1
    Checksum:i3EB05150B80CDE95
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY772189 mRNA. Translation: AAV40998.1.
    AC108758 Genomic DNA. No translation available.
    AP008215 Genomic DNA. Translation: BAF25444.1.
    AP014965 Genomic DNA. Translation: BAT08714.1.
    AK063214 mRNA. Translation: BAG88597.1.
    RefSeqiXP_015610795.1. XM_015755309.1.
    UniGeneiOs.88036.

    Genome annotation databases

    EnsemblPlantsiOS09T0488000-01; OS09T0488000-01; OS09G0488000.
    GeneIDi4347432.
    GrameneiOS09T0488000-01; OS09T0488000-01; OS09G0488000.
    KEGGiosa:4347432.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY772189 mRNA. Translation: AAV40998.1.
    AC108758 Genomic DNA. No translation available.
    AP008215 Genomic DNA. Translation: BAF25444.1.
    AP014965 Genomic DNA. Translation: BAT08714.1.
    AK063214 mRNA. Translation: BAG88597.1.
    RefSeqiXP_015610795.1. XM_015755309.1.
    UniGeneiOs.88036.

    3D structure databases

    ProteinModelPortaliQ5U9F2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi39947.LOC_Os09g31310.1.

    Proteomic databases

    PaxDbiQ5U9F2.
    PRIDEiQ5U9F2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiOS09T0488000-01; OS09T0488000-01; OS09G0488000.
    GeneIDi4347432.
    GrameneiOS09T0488000-01; OS09T0488000-01; OS09G0488000.
    KEGGiosa:4347432.

    Phylogenomic databases

    eggNOGiKOG3396. Eukaryota.
    COG0454. LUCA.
    InParanoidiQ5U9F2.
    KOiK00621.
    OMAiKFIHNCA.

    Enzyme and pathway databases

    UniPathwayiUPA00113; UER00529.
    ReactomeiR-OSA-446210. Synthesis of UDP-N-acetyl-glucosamine.

    Gene expression databases

    GenevisibleiQ5U9F2. OS.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view]
    PfamiPF00583. Acetyltransf_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "A novel short-root gene encodes a glucosamine-6-phosphate acetyltransferase required for maintaining normal root cell shape in rice."
      Jiang H., Wang S., Dang L., Wang S., Chen H., Wu Y., Jiang X., Wu P.
      Plant Physiol. 138:232-242(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiGNA1_ORYSJ
    AccessioniPrimary (citable) accession number: Q5U9F2
    Secondary accession number(s): A0A0N7KR10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2013
    Last sequence update: December 7, 2004
    Last modified: May 11, 2016
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.