Reviewed, UniProtKB/Swiss-Prot Q5U907 (MDH_ACTSZ)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Asuc_1612

Organism

Actinobacillus succinogenes (strain ATCC 55618 / 130Z) [Complete proteome] [HAMAP]

Taxonomic identifier

339671 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01516
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Malate dehydrogenase HAMAP MF_01516		PRO_0000113299
Regions
	Nucleotide binding	7 – 13	7	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	227	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5U907-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 029DEF60E6D2106A	FASTA	312	33,115
	10 20 30 40 50 60 MKVTLLGASG GIGQPLSLLL KLHLPAESDL SLYDVAPVTP GVAKDISHIP TSVEVEGFGG 70 80 90 100 110 120 DDPSEALKGA DIVLICAGVA RKPGMTRADL FNVNAGIIQN LVEKVAQVCP QACVCIITNP 130 140 150 160 170 180 VNSIIPIAAE VLKKAGVYDK RKLFGITTLD TIRSEKFIVQ AKNIEINRND ISVIGGHSGV 190 200 210 220 230 240 TILPLLSQIP HVEFTEQELK DLTHRIQNAG TEVVEAKAGA GSATLSMAYA AMRFVVSMAR 250 260 270 280 290 300 ALNGEVITEC AYIEGDGKFA RFFAQPVRLG KNGVEEILPL GTLSAFEQQA LEAMLPTLQT 310 DIDNGVKFVT GE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Actinobacillus succinogenes mdh gene cloning and recombinant enzyme characterization." Laivenieks M., Vieille C., Zeikus J.G. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Actinobacillus succinogenes 130Z." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY773261 Genomic DNA. Translation: AAV41054.1. CP000746 Genomic DNA. Translation: ABR74966.1.
RefSeq	YP_001344901.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5348645.
GenomeReviews	Gene locus Asuc_1612 in contig CP000746_GR.
KEGG	asu:Asuc_1612.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q5U907. YLPGLPG.
Family and domain databases
HAMAP	MF_01516. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_ACTSZ

Accession

Primary (citable) accession number: Q5U907
Secondary accession number(s): A6VPR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)