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Protein

Carboxypeptidase A6

Gene

Cpa6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in the proteolytic inactivation of enkephalins and neurotensin in some brain areas. May convert inactive angiotensin I into the biologically active angiotensin II (By similarity).By similarity

Catalytic activityi

Release of a C-terminal amino acid, with preference for large hydrophobic C-terminal amino acids and only very weak activity toward small amino acids and histidine.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi197Zinc; catalyticBy similarity1
Metal bindingi200Zinc; catalyticBy similarity1
Binding sitei255SubstrateBy similarity1
Metal bindingi325Zinc; catalyticBy similarity1
Binding sitei377SubstrateBy similarity1
Active sitei399Proton donor/acceptorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.1. 3474.

Protein family/group databases

MEROPSiM14.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase A6 (EC:3.4.17.-)
Gene namesi
Name:Cpa6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:3045348. Cpa6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
PropeptideiPRO_000000436731 – 129Activation peptideBy similarityAdd BLAST99
ChainiPRO_0000004368130 – 438Carboxypeptidase A6Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Glycosylationi153N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi266 ↔ 289By similarity
Glycosylationi344N-linked (GlcNAc...)Sequence analysis1
Glycosylationi428N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ5U901.
PRIDEiQ5U901.

PTM databases

PhosphoSitePlusiQ5U901.

Expressioni

Tissue specificityi

In brain, highly expressed in the olfactory bulb with lower levels in other regions including cerebral cortex, hippocampus, hypothalamus, striatum and medulla. Within the olfactory bulb, highest levels occur in the mitral and granular layers with lower levels in the internal and external plexiform layers. Moderate levels are found in the epididymis with low levels in colon and spleen. Not detected in adrenal, liver, lung, ovary or testis. At embryonic day 14.5, enriched in eye, ear, osteoblasts, stomach, skin, dorsal root ganglia and throughout the CNS.1 Publication

Gene expression databases

BgeeiENSMUSG00000042501.
CleanExiMM_CPA6.
ExpressionAtlasiQ5U901. baseline and differential.
GenevisibleiQ5U901. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035435.

Structurei

3D structure databases

ProteinModelPortaliQ5U901.
SMRiQ5U901.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 200Substrate bindingBy similarity4
Regioni272 – 273Substrate bindingBy similarity2
Regioni326 – 327Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiQ5U901.
KOiK08782.
OMAiRKHIRAY.
OrthoDBiEOG091G0HUI.
PhylomeDBiQ5U901.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5U901-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNFLGNPRSH TAAFLPVCWL LLNILKPGHC HSYDNRYAGD KVIRLIPKSE
60 70 80 90 100
EEALALKNIY HQLKVDLWQP SSISYVSEGT ITDVHISQNA SRTLLAFLQE
110 120 130 140 150
THIYYKVLIE DLQKAVENEN SLQTQRNRRS LSEYNYEVYH SLEDIQSWLH
160 170 180 190 200
HLNQTQPGLV RVFSIGRSYE GRPLFIMQLG RKSRAYKRAV WIDCGIHARE
210 220 230 240 250
WIGPAFCQWF VREAILTYKT DPAMKKMLNH LYFYIMPVFN VDGYHFSWTH
260 270 280 290 300
DRFWRKTRSR DSKFRCRGVD ANRNWKVKWC DEGASAHPCD DTYCGPFPES
310 320 330 340 350
EPEVKAVANF LRKHRKRIRA YLSFHAYAQM LLYPYSYKYA TIPNFSCVEF
360 370 380 390 400
AAHKAVKALR SVHGIRYRHG PASQTLYVSS GNSMDWAYKN GIPYAFAFEL
410 420 430
RDTGHFGFLL PEMLIKPTCT ETMLAVKNIT MHLLKKCP
Length:438
Mass (Da):51,143
Last modified:December 7, 2004 - v1
Checksum:iA6CB743AEFEFA18B
GO
Isoform 2 (identifier: Q5U901-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-176: Missing.

Note: No experimental confirmation available.
Show »
Length:262
Mass (Da):30,709
Checksum:iD85744A6767FAE7A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0170811 – 176Missing in isoform 2. 1 PublicationAdd BLAST176

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY773477 mRNA. Translation: AAV40814.1.
AK078883 mRNA. Translation: BAC37442.1.
CCDSiCCDS14819.1. [Q5U901-1]
RefSeqiNP_001276426.1. NM_001289497.1. [Q5U901-2]
NP_808502.2. NM_177834.4. [Q5U901-1]
UniGeneiMm.336423.

Genome annotation databases

EnsembliENSMUST00000035577; ENSMUSP00000035435; ENSMUSG00000042501. [Q5U901-1]
GeneIDi329093.
KEGGimmu:329093.
UCSCiuc007ahq.2. mouse. [Q5U901-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY773477 mRNA. Translation: AAV40814.1.
AK078883 mRNA. Translation: BAC37442.1.
CCDSiCCDS14819.1. [Q5U901-1]
RefSeqiNP_001276426.1. NM_001289497.1. [Q5U901-2]
NP_808502.2. NM_177834.4. [Q5U901-1]
UniGeneiMm.336423.

3D structure databases

ProteinModelPortaliQ5U901.
SMRiQ5U901.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035435.

Protein family/group databases

MEROPSiM14.018.

PTM databases

PhosphoSitePlusiQ5U901.

Proteomic databases

PaxDbiQ5U901.
PRIDEiQ5U901.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035577; ENSMUSP00000035435; ENSMUSG00000042501. [Q5U901-1]
GeneIDi329093.
KEGGimmu:329093.
UCSCiuc007ahq.2. mouse. [Q5U901-1]

Organism-specific databases

CTDi57094.
MGIiMGI:3045348. Cpa6.

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiQ5U901.
KOiK08782.
OMAiRKHIRAY.
OrthoDBiEOG091G0HUI.
PhylomeDBiQ5U901.
TreeFamiTF317197.

Enzyme and pathway databases

BRENDAi3.4.17.1. 3474.

Miscellaneous databases

PROiQ5U901.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042501.
CleanExiMM_CPA6.
ExpressionAtlasiQ5U901. baseline and differential.
GenevisibleiQ5U901. MM.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPA6_MOUSE
AccessioniPrimary (citable) accession number: Q5U901
Secondary accession number(s): Q8BVD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.