Q5U777 (APR_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phorbol-12-myristate-13-acetate-induced protein 1

Alternative name(s):
Protein Noxa

Gene names

Name:	Pmaip1
Synonyms:	Noxa

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 and with BIM/BCL2L11 for binding to MCL1; can displace BAK1 and BIM/BCL2L11 from their binding sites By similarity.
Subunit structure	Interacts with MCL1, BCL2A1 and BAX By similarity.
Subcellular location	Mitochondrion By similarity.
Domain	The BH3 motif is essential for pro-apoptotic activity By similarity.
Sequence similarities	Belongs to the PMAIP1 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Mitochondrion
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell homeostasis Inferred from electronic annotation. Source: Compara activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara apoptotic process Inferred from expression pattern PubMed 18959742. Source: RGD cellular component disassembly involved in execution phase of apoptosis Inferred from electronic annotation. Source: Compara cellular response to glucose starvation Inferred from electronic annotation. Source: Compara cellular response to hypoxia Inferred from electronic annotation. Source: Compara induction of apoptosis Inferred from electronic annotation. Source: Compara intrinsic apoptotic signaling pathway by p53 class mediator Inferred from electronic annotation. Source: Compara negative regulation of fibroblast proliferation Inferred from electronic annotation. Source: Compara negative regulation of mitochondrial membrane potential Inferred from electronic annotation. Source: Compara positive regulation of DNA damage response, signal transduction by p53 class mediator Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from mutant phenotype PubMed 18614532. Source: RGD positive regulation of glucose metabolic process Inferred from electronic annotation. Source: Compara positive regulation of intrinsic apoptotic signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of protein oligomerization Inferred from electronic annotation. Source: Compara positive regulation of release of cytochrome c from mitochondria Inferred from electronic annotation. Source: Compara proteasomal protein catabolic process Inferred from electronic annotation. Source: Compara reactive oxygen species metabolic process Inferred from electronic annotation. Source: Compara regulation of mitochondrial membrane permeability Inferred from electronic annotation. Source: Compara release of cytochrome c from mitochondria Inferred from electronic annotation. Source: Compara response to DNA damage stimulus Inferred from electronic annotation. Source: Compara response to UV Inferred from electronic annotation. Source: Compara response to X-ray Inferred from electronic annotation. Source: Compara response to dsRNA Inferred from electronic annotation. Source: Compara virus-infected cell apoptotic process Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara mitochondrion Inferred from direct assay PubMed 17127418. Source: RGD nucleus Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Phorbol-12-myristate-13-acetate-induced protein 1		PRO_0000333231
Regions
	Region	92 – 101	10	Required for mitochondrial location By similarity
	Motif	27 – 35	9	BH3 1
	Motif	80 – 88	9	BH3 2

Sequences

	Sequence		Length	Mass (Da)
	Q5U777 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 76EF4EE17827890E	FASTA	105	11,901
	10 20 30 40 50 60 MPGRKARRNA PLNPTRAELP PEFAAQLRKI GDKVYCTWSA PDITAVLAQM PGKKSRKSTM 70 80 90 100 RRSPSPTRVP ADLKDECDQL RRIGDKVNLR QKLLNFISKL FNLIT

References

[1]	Yakovlev A.G. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Embryonic brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY788892 mRNA. Translation: AAV51955.1.
IPI	IPI00193637.
RefSeq	NP_001008386.1. NM_001008385.1.
UniGene	Rn.163380.
3D structure databases
ProteinModelPortal	Q5U777.
SMR	Q5U777. Positions 17-43, 70-95.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000025361.
PTM databases
PhosphoSite	Q5U777.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025362; ENSRNOP00000025361; ENSRNOG00000018770.
GeneID	492821.
KEGG	rno:492821.
UCSC	RGD:1359266. rat.
Organism-specific databases
CTD	5366.
RGD	1359266. Pmaip1.
Phylogenomic databases
eggNOG	NOG119326.
GeneTree	ENSGT00530000065105.
HOGENOM	HOG000034021.
HOVERGEN	HBG095669.
InParanoid	Q5U777.
KO	K10131.
OMA	RKARRNA.
OrthoDB	EOG4QC16X.
Gene expression databases
Genevestigator	Q5U777.
Family and domain databases
InterPro	IPR024140. PMA-induced_Noxa. [Graphical view]
PANTHER	PTHR14299. PTHR14299. 1 hit.
PROSITE	PS01259. BH3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	697216.

Entry information

Entry name

APR_RAT

Accession

Primary (citable) accession number: Q5U777

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 7, 2004
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program