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Protein

Ras-interacting protein 1

Gene

RASIP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy.By similarity2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Angiogenesis

Enzyme and pathway databases

SignaLinkiQ5U651.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-interacting protein 1
Short name:
Rain
Gene namesi
Name:RASIP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:24716. RASIP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134875519.

Polymorphism and mutation databases

BioMutaiRASIP1.
DMDMi74736209.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 963963Ras-interacting protein 1PRO_0000097163Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei188 – 1881PhosphoserineCombined sources
Modified residuei280 – 2801PhosphoserineBy similarity
Modified residuei292 – 2921PhosphoserineBy similarity
Modified residuei326 – 3261PhosphoserineBy similarity
Modified residuei328 – 3281PhosphoserineBy similarity
Modified residuei331 – 3311PhosphoserineBy similarity
Modified residuei419 – 4191PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5U651.
MaxQBiQ5U651.
PaxDbiQ5U651.
PeptideAtlasiQ5U651.
PRIDEiQ5U651.

PTM databases

iPTMnetiQ5U651.
PhosphoSiteiQ5U651.

Expressioni

Tissue specificityi

Highly expressed in heart. Detected at lower levels in placenta and pancreas.1 Publication

Gene expression databases

BgeeiQ5U651.
CleanExiHS_RASIP1.
ExpressionAtlasiQ5U651. baseline and differential.
GenevisibleiQ5U651. HS.

Interactioni

Subunit structurei

Interacts with Ras family members that have been activated by GTP binding. Interacts with HRAS, RAP1A, RAP2, RRAS, RAF1 and RRAS2. Interacts with MYH9 and ARHGAP29 (By similarity).By similarity

Protein-protein interaction databases

BioGridi120263. 17 interactions.
IntActiQ5U651. 16 interactions.
STRINGi9606.ENSP00000222145.

Structurei

3D structure databases

ProteinModelPortaliQ5U651.
SMRiQ5U651. Positions 137-263, 544-881.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini144 – 259116Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini600 – 897298DilutePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi59 – 657Poly-Pro

Sequence similaritiesi

Contains 1 dilute domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0160. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00530000063674.
HOGENOMiHOG000154071.
HOVERGENiHBG079536.
InParanoidiQ5U651.
OMAiQELPANY.
OrthoDBiEOG7ZSHS8.
PhylomeDBiQ5U651.
TreeFamiTF315987.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR002710. Dilute_dom.
IPR000253. FHA_dom.
IPR000159. RA_dom.
IPR008984. SMAD_FHA_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM01132. DIL. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51126. DILUTE. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSGERKEGG SPRFGKLHLP VGLWINSPRK QLAKLGRRWP SAASVKSSSS
60 70 80 90 100
DTGSRSSEPL PPPPPHVELR RVGAVKAAGG ASGSRAKRIS QLFRGSGTGT
110 120 130 140 150
TGSSGAGGPG TPGGAQRWAS EKKLPELAAG VAPEPPLATR ATAPPGVLKI
160 170 180 190 200
FGAGLASGAN YKSVLATARS TARELVAEAL ERYGLAGSPG GGPGESSCVD
210 220 230 240 250
AFALCDALGR PAAAGVGSGE WRAEHLRVLG DSERPLLVQE LWRARPGWAR
260 270 280 290 300
RFELRGREEA RRLEQEAFGA ADSEGTGAPS WRPQKNRSRA ASGGAALASP
310 320 330 340 350
GPGTGSGAPA GSGGKERSEN LSLRRSVSEL SLQGRRRRQQ ERRQQALSMA
360 370 380 390 400
PGAADAQIGT ADPGDFDQLT QCLIQAPSNR PYFLLLQGYQ DAQDFVVYVM
410 420 430 440 450
TREQHVFGRG GNSSGRGGSP APYVDTFLNA PDILPRHCTV RAGPEHPAMV
460 470 480 490 500
RPSRGAPVTH NGCLLLREAE LHPGDLLGLG EHFLFMYKDP RTGGSGPARP
510 520 530 540 550
PWLPARPGAT PPGPGWAFSC RLCGRGLQER GEALAAYLDG REPVLRFRPR
560 570 580 590 600
EEEALLGEIV RAAAAGSGDL PPLGPATLLA LCVQHSAREL ELGHLPRLLG
610 620 630 640 650
RLARLIKEAV WEKIKEIGDR QPENHPEGVP EVPLTPEAVS VELRPLMLWM
660 670 680 690 700
ANTTELLSFV QEKVLEMEKE ADQEDPQLCN DLELCDEAMA LLDEVIMCTF
710 720 730 740 750
QQSVYYLTKT LYSTLPALLD SNPFTAGAEL PGPGAELGAM PPGLRPTLGV
760 770 780 790 800
FQAALELTSQ CELHPDLVSQ TFGYLFFFSN ASLLNSLMER GQGRPFYQWS
810 820 830 840 850
RAVQIRTNLD LVLDWLQGAG LGDIATEFFR KLSMAVNLLC VPRTSLLKAS
860 870 880 890 900
WSSLRTDHPT LTPAQLHHLL SHYQLGPGRG PPAAWDPPPA EREAVDTGDI
910 920 930 940 950
FESFSSHPPL ILPLGSSRLR LTGPVTDDAL HRELRRLRRL LWDLEQQELP
960
ANYRHGPPVA TSP
Length:963
Mass (Da):103,457
Last modified:December 7, 2004 - v1
Checksum:i470021F91B59EC52
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti500 – 5001P → R in AAR24580 (PubMed:15031288).Curated
Sequence conflicti511 – 5177PPGPGWA → RQPWLG in AAR24580 (PubMed:15031288).Curated
Sequence conflicti736 – 7372EL → DW in AAR24580 (PubMed:15031288).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti601 – 6011R → C.1 Publication
Corresponds to variant rs2287922 [ dbSNP | Ensembl ].
VAR_051302

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY378097 mRNA. Translation: AAR24580.1.
BC028614 mRNA. Translation: AAH28614.1.
CCDSiCCDS12731.1.
RefSeqiNP_060275.2. NM_017805.2.
UniGeneiHs.233955.

Genome annotation databases

EnsembliENST00000222145; ENSP00000222145; ENSG00000105538.
GeneIDi54922.
KEGGihsa:54922.
UCSCiuc002pki.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY378097 mRNA. Translation: AAR24580.1.
BC028614 mRNA. Translation: AAH28614.1.
CCDSiCCDS12731.1.
RefSeqiNP_060275.2. NM_017805.2.
UniGeneiHs.233955.

3D structure databases

ProteinModelPortaliQ5U651.
SMRiQ5U651. Positions 137-263, 544-881.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120263. 17 interactions.
IntActiQ5U651. 16 interactions.
STRINGi9606.ENSP00000222145.

PTM databases

iPTMnetiQ5U651.
PhosphoSiteiQ5U651.

Polymorphism and mutation databases

BioMutaiRASIP1.
DMDMi74736209.

Proteomic databases

EPDiQ5U651.
MaxQBiQ5U651.
PaxDbiQ5U651.
PeptideAtlasiQ5U651.
PRIDEiQ5U651.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000222145; ENSP00000222145; ENSG00000105538.
GeneIDi54922.
KEGGihsa:54922.
UCSCiuc002pki.4. human.

Organism-specific databases

CTDi54922.
GeneCardsiRASIP1.
HGNCiHGNC:24716. RASIP1.
MIMi609623. gene.
neXtProtiNX_Q5U651.
PharmGKBiPA134875519.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0160. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00530000063674.
HOGENOMiHOG000154071.
HOVERGENiHBG079536.
InParanoidiQ5U651.
OMAiQELPANY.
OrthoDBiEOG7ZSHS8.
PhylomeDBiQ5U651.
TreeFamiTF315987.

Enzyme and pathway databases

SignaLinkiQ5U651.

Miscellaneous databases

ChiTaRSiRASIP1. human.
GenomeRNAii54922.
PROiQ5U651.
SOURCEiSearch...

Gene expression databases

BgeeiQ5U651.
CleanExiHS_RASIP1.
ExpressionAtlasiQ5U651. baseline and differential.
GenevisibleiQ5U651. HS.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR002710. Dilute_dom.
IPR000253. FHA_dom.
IPR000159. RA_dom.
IPR008984. SMAD_FHA_domain.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM01132. DIL. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51126. DILUTE. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of Rain, a novel Ras-interacting protein with a unique subcellular localization."
    Mitin N.Y., Ramocki M.B., Zullo A.J., Der C.J., Konieczny S.F., Taparowsky E.J.
    J. Biol. Chem. 279:22353-22361(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH HRAS; RAP1A; RAP2; RAF1; RRAS AND RRAS2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANT CYS-601.
    Tissue: Skeletal muscle.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC."
    McKnight N.C., Jefferies H.B., Alemu E.A., Saunders R.E., Howell M., Johansen T., Tooze S.A.
    EMBO J. 31:1931-1946(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188 AND SER-419, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiRAIN_HUMAN
AccessioniPrimary (citable) accession number: Q5U651
Secondary accession number(s): Q6U676
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: December 7, 2004
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.