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Q5U623 (MCAF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Activating transcription factor 7-interacting protein 2
Short name=ATF7-interacting protein 2
Alternative name(s):
MBD1-containing chromatin-associated factor 2
Gene names
Name:ATF7IP2
Synonyms:MCAF2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length682 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) activity Probable.

Subunit structure

Interacts with MBD1, SETDB1 and SP1. Probably forms a complex with SETDB1 and MBD1.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the MCAF family.

Contains 1 fibronectin type-III domain.

Sequence caution

The sequence BAB14209.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainCoiled coil
Repeat
   Molecular functionActivator
Repressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processregulation of transcription, DNA-dependent

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-dependent

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from direct assay. Source: HPA

nucleolus

Inferred from direct assay. Source: HPA

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5U623-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5U623-2)

The sequence of this isoform differs from the canonical sequence as follows:
     495-543: AVQKKLDSII...KETTPLAQNA → KVQYPPWSHI...SQNHQHHYLN
     544-682: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 682682Activating transcription factor 7-interacting protein 2
PRO_0000281784

Regions

Domain572 – 674103Fibronectin type-III
Coiled coil328 – 37851 Potential

Amino acid modifications

Modified residue4881Phosphoserine Ref.6

Natural variations

Alternative sequence495 – 54349AVQKK…LAQNA → KVQYPPWSHIRKLLQTQRKQ PHWHKMQSRFLSPLSTCHLS QNHQHHYLN in isoform 2.
VSP_024042
Alternative sequence544 – 682139Missing in isoform 2.
VSP_024043
Natural variant5271S → L.
Corresponds to variant rs34834862 [ dbSNP | Ensembl ].
VAR_053872
Natural variant5371T → I.
Corresponds to variant rs9932051 [ dbSNP | Ensembl ].
VAR_031286
Natural variant5431A → T.
Corresponds to variant rs9931441 [ dbSNP | Ensembl ].
VAR_031287

Experimental info

Sequence conflict611Missing in AAT66299. Ref.1
Sequence conflict611Missing in AAH33891. Ref.3
Sequence conflict4701I → T in BAB14209. Ref.2
Sequence conflict5551P → L in BAD97212. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 3, 2007. Version 2.
Checksum: 2FF5417BB7058E31

FASTA68275,764
        10         20         30         40         50         60 
MASPDRSKRK ILKAKKTMPL SCRKQVEMLN KSRNVEALKT AIGSNVPSGN QSFSPSVITR 

        70         80         90        100        110        120 
TTEITKCSPS ENGASSLDSN KNSISEKSKV FSQNCIKPVE EIVHSETKLE QVVCSYQKPS 

       130        140        150        160        170        180 
RTTESPSRVF TEEAKDSLNT SENDSEHQTN VTRSLFEHEG ACSLKSSCCP PSVLSGVVQM 

       190        200        210        220        230        240 
PESTVTSTVG DKKTDQMVFH LETNSNSESH DKRQSDNILC SEDSGFVPVE KTPNLVNSVT 

       250        260        270        280        290        300 
SNNCADDILK TDECSRTSIS NCESADSTWQ SSLDTNNNSH YQKKRMFSEN EENVKRMKTS 

       310        320        330        340        350        360 
EQINENICVS LERQTAFLEQ VRHLIQQEIY SINYELFDKK LKELNQRIGK TECRNKHEGI 

       370        380        390        400        410        420 
ADKLLAKIAK LQRRIKTVLL FQRNCLKPNM LSSNGASKVA NSEAMILDKN LESVNSPIEK 

       430        440        450        460        470        480 
SSVNYEPSNP SEKGSKKINL SSDQNKSVSE SNNDDVMLIS VESPNLTTPI TSNPTDTRKI 

       490        500        510        520        530        540 
TSGNSSNSPN AEVMAVQKKL DSIIDLTKEG LSNCNTESPV SPLESHSKAA SNSKETTPLA 

       550        560        570        580        590        600 
QNAVQVPESF EHLPPLPEPP APLPELVDKT RDTLPPQKPE LKVKRVFRPN GIALTWNITK 

       610        620        630        640        650        660 
INPKCAPVES YHLFLCHENS NNKLIWKKIG EIKALPLPMA CTLSQFLASN RYYFTVQSKD 

       670        680 
IFGRYGPFCD IKSIPGFSEN LT

« Hide

Isoform 2 [UniParc].

Checksum: 392D2912125794A4
Show »

FASTA54360,894

References

« Hide 'large scale' references
[1]"Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins."
Ichimura T., Watanabe S., Sakamoto Y., Aoto T., Fujita N., Nakao M.
J. Biol. Chem. 280:13928-13935(2005) [PubMed: 15691849] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 339-682 (ISOFORM 1).
Tissue: Teratocarcinoma and Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung and Testis.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 19-398 (ISOFORM 1).
Tissue: Stomach.
[5]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 324-681 (ISOFORM 1).
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488, MASS SPECTROMETRY.
Tissue: Embryonic kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY560615 mRNA. Translation: AAT66299.1.
AK022730 mRNA. Translation: BAB14209.1. Different initiation.
AK093414 mRNA. Translation: BAC04157.1.
BC033891 mRNA. Translation: AAH33891.1.
BC069713 mRNA. Translation: AAH69713.1.
BC069730 mRNA. Translation: AAH69730.1.
BC069695 mRNA. Translation: AAH69695.1.
BC137079 mRNA. Translation: AAI37080.1.
AL832987 mRNA. Translation: CAH56323.1.
AK223492 mRNA. Translation: BAD97212.1.
IPIIPI00167841.
IPI00411827.
RefSeqNP_079273.2. NM_024997.2.
UniGeneHs.513343.

3D structure databases

ProteinModelPortalQ5U623.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5U623.

Polymorphism databases

DMDM143352739.

Proteomic databases

PRIDEQ5U623.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356427; ENSP00000348799; ENSG00000166669.
ENST00000396560; ENSP00000379808; ENSG00000166669.
GeneID80063.
KEGGhsa:80063.
UCSCuc002czu.1. human.
uc002czv.1. human.

Organism-specific databases

CTD80063.
GeneCardsGC16P010430.
H-InvDBHIX0012811.
HGNCHGNC:20397. ATF7IP2.
HPAHPA041156.
HPA041228.
MIM613645. gene.
neXtProtNX_Q5U623.
PharmGKBPA134988230.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG16404.
GeneTreeENSGT00530000063707.
HOVERGENHBG094861.
InParanoidQ5U623.
OMAVPESFEH.
PhylomeDBQ5U623.

Gene expression databases

ArrayExpressQ5U623.
BgeeQ5U623.
CleanExHS_ATF7IP2.
GenevestigatorQ5U623.

Family and domain databases

PROSITEPS50853. FN3. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio70263.
SOURCESearch...

Entry information

Entry nameMCAF2_HUMAN
AccessionPrimary (citable) accession number: Q5U623
Secondary accession number(s): B2RNR2 expand/collapse secondary AC list , Q53EZ7, Q658U2, Q6IS97, Q8N9X8, Q9H9L6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: January 25, 2012
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents