Reviewed, UniProtKB/Swiss-Prot Q5U623 (MCAF2_HUMAN)

Names and origin

Protein names

Recommended name:
    Activating transcription factor 7-interacting protein 2
Alternative name(s):
    MBD1-containing chromatin-associated factor 2

Gene names

Name:	ATF7IP2
Synonyms:	MCAF2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	682 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone 'Lys-9' trimethylation activity Probable.
Subunit structure	Interacts with MBD1, SETDB1 and SP1. Probably forms a complex with SETDB1 and MBD1.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the MCAF family. Contains 1 fibronectin type-III domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5U623-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5U623-2)

The sequence of this isoform differs from the canonical sequence as follows:
     495-543: AVQKKLDSII...KETTPLAQNA → KVQYPPWSHI...SQNHQHHYLN
     544-682: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 682	682	Activating transcription factor 7-interacting protein 2		PRO_0000281784
Regions
	Domain	572 – 674	103	Fibronectin type-III
	Coiled coil	328 – 378	51	Potential
Amino acid modifications
	Modified residue	488	1	Phosphoserine
Natural variations
	Alternative sequence	495 – 543	49	AVQKK…LAQNA → KVQYPPWSHIRKLLQTQRKQ PHWHKMQSRFLSPLSTCHLS QNHQHHYLN in isoform 2.		VSP_024042
	Alternative sequence	544 – 682	139	Missing in isoform 2.		VSP_024043
	Natural variant	527	1	S → L: dbSNP rs34834862.		VAR_053872
	Natural variant	537	1	T → I: dbSNP rs9932051.		VAR_031286
	Natural variant	543	1	A → T: dbSNP rs9931441.		VAR_031287
Experimental info
	Sequence conflict	61	1	Missing in AAT66299. Ref.1
	Sequence conflict	61	1	Missing in AAH33891. Ref.3
	Sequence conflict	470	1	I → T in BAB14209. Ref.2
	Sequence conflict	555	1	P → L in BAD97212. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 2FF5417BB7058E31	FASTA	682	75,764
	10 20 30 40 50 60 MASPDRSKRK ILKAKKTMPL SCRKQVEMLN KSRNVEALKT AIGSNVPSGN QSFSPSVITR 70 80 90 100 110 120 TTEITKCSPS ENGASSLDSN KNSISEKSKV FSQNCIKPVE EIVHSETKLE QVVCSYQKPS 130 140 150 160 170 180 RTTESPSRVF TEEAKDSLNT SENDSEHQTN VTRSLFEHEG ACSLKSSCCP PSVLSGVVQM 190 200 210 220 230 240 PESTVTSTVG DKKTDQMVFH LETNSNSESH DKRQSDNILC SEDSGFVPVE KTPNLVNSVT 250 260 270 280 290 300 SNNCADDILK TDECSRTSIS NCESADSTWQ SSLDTNNNSH YQKKRMFSEN EENVKRMKTS 310 320 330 340 350 360 EQINENICVS LERQTAFLEQ VRHLIQQEIY SINYELFDKK LKELNQRIGK TECRNKHEGI 370 380 390 400 410 420 ADKLLAKIAK LQRRIKTVLL FQRNCLKPNM LSSNGASKVA NSEAMILDKN LESVNSPIEK 430 440 450 460 470 480 SSVNYEPSNP SEKGSKKINL SSDQNKSVSE SNNDDVMLIS VESPNLTTPI TSNPTDTRKI 490 500 510 520 530 540 TSGNSSNSPN AEVMAVQKKL DSIIDLTKEG LSNCNTESPV SPLESHSKAA SNSKETTPLA 550 560 570 580 590 600 QNAVQVPESF EHLPPLPEPP APLPELVDKT RDTLPPQKPE LKVKRVFRPN GIALTWNITK 610 620 630 640 650 660 INPKCAPVES YHLFLCHENS NNKLIWKKIG EIKALPLPMA CTLSQFLASN RYYFTVQSKD 670 680 IFGRYGPFCD IKSIPGFSEN LT
	Isoform 2. Checksum: 392D2912125794A4	FASTA	543	60,894
	10 20 30 40 50 60 MASPDRSKRK ILKAKKTMPL SCRKQVEMLN KSRNVEALKT AIGSNVPSGN QSFSPSVITR 70 80 90 100 110 120 TTEITKCSPS ENGASSLDSN KNSISEKSKV FSQNCIKPVE EIVHSETKLE QVVCSYQKPS 130 140 150 160 170 180 RTTESPSRVF TEEAKDSLNT SENDSEHQTN VTRSLFEHEG ACSLKSSCCP PSVLSGVVQM 190 200 210 220 230 240 PESTVTSTVG DKKTDQMVFH LETNSNSESH DKRQSDNILC SEDSGFVPVE KTPNLVNSVT 250 260 270 280 290 300 SNNCADDILK TDECSRTSIS NCESADSTWQ SSLDTNNNSH YQKKRMFSEN EENVKRMKTS 310 320 330 340 350 360 EQINENICVS LERQTAFLEQ VRHLIQQEIY SINYELFDKK LKELNQRIGK TECRNKHEGI 370 380 390 400 410 420 ADKLLAKIAK LQRRIKTVLL FQRNCLKPNM LSSNGASKVA NSEAMILDKN LESVNSPIEK 430 440 450 460 470 480 SSVNYEPSNP SEKGSKKINL SSDQNKSVSE SNNDDVMLIS VESPNLTTPI TSNPTDTRKI 490 500 510 520 530 540 TSGNSSNSPN AEVMKVQYPP WSHIRKLLQT QRKQPHWHKM QSRFLSPLST CHLSQNHQHH YLN

References

[1]	"Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins." Ichimura T., Watanabe S., Sakamoto Y., Aoto T., Fujita N., Nakao M. J. Biol. Chem. 280:13928-13935(2005) [PubMed: 15691849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 339-682 (ISOFORM 1). Tissue: Teratocarcinoma and Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Testis.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 19-398 (ISOFORM 1). Tissue: Stomach.
[5]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 324-681 (ISOFORM 1).
[6]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY560615 mRNA. Translation: AAT66299.1. AK022730 mRNA. Translation: BAB14209.1. Different initiation. AK093414 mRNA. Translation: BAC04157.1. BC033891 mRNA. Translation: AAH33891.1. BC069713 mRNA. Translation: AAH69713.1. BC069730 mRNA. Translation: AAH69730.1. BC069695 mRNA. Translation: AAH69695.1. BC137079 mRNA. Translation: AAI37080.1. AL832987 mRNA. Translation: CAH56323.1. AK223492 mRNA. Translation: BAD97212.1.
IPI	IPI00167841. IPI00411827.
RefSeq	NP_079273.2.
UniGene	Hs.513343
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5U623.
Proteomic databases
PRIDE	Q5U623.
Genome annotation databases
Ensembl	ENST00000356427; ENSP00000348799; ENSG00000166669; Homo sapiens. [Genome view] ENST00000396560; ENSP00000379808; ENSG00000166669; Homo sapiens. [Genome view]
GeneID	80063.
KEGG	hsa:80063.
UCSC	uc002czu.1. human. uc002czv.1. human.
Organism-specific databases
CTD	80063.
GeneCards	GC16P010430.
H-InvDB	HIX0012811.
HGNC	HGNC:20397. ATF7IP2.
PharmGKB	PA134988230.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q5U623.
OMA	FLCHENS
Gene expression databases
ArrayExpress	Q5U623.
Bgee	Q5U623.
CleanEx	HS_ATF7IP2.
Genevestigator	Q5U623.
Family and domain databases
PROSITE	PS50853. FN3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	70263.

Entry information

Entry name

MCAF2_HUMAN

Accession

Primary (citable) accession number: Q5U623
Secondary accession number(s): B2RNR2 , Q53EZ7, Q658U2, Q6IS97, Q8N9X8, Q9H9L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	November 24, 2009
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.