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Protein

Probable E3 ubiquitin-protein ligase HECTD2

Gene

HECTD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei744Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase HECTD2 (EC:2.3.2.26)
Alternative name(s):
HECT domain-containing protein 2
HECT-type E3 ubiquitin transferase HECTD2
Gene namesi
Name:HECTD2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:26736. HECTD2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi143279.
OpenTargetsiENSG00000165338.
PharmGKBiPA134933711.

Polymorphism and mutation databases

DMDMi109892196.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002408511 – 776Probable E3 ubiquitin-protein ligase HECTD2Add BLAST776

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5U5R9.
PaxDbiQ5U5R9.
PeptideAtlasiQ5U5R9.
PRIDEiQ5U5R9.

PTM databases

iPTMnetiQ5U5R9.
PhosphoSitePlusiQ5U5R9.

Expressioni

Gene expression databases

BgeeiENSG00000165338.
CleanExiHS_HECTD2.
ExpressionAtlasiQ5U5R9. baseline and differential.
GenevisibleiQ5U5R9. HS.

Organism-specific databases

HPAiHPA037767.

Interactioni

Protein-protein interaction databases

BioGridi126794. 4 interactors.
IntActiQ5U5R9. 1 interactor.
STRINGi9606.ENSP00000298068.

Structurei

3D structure databases

ProteinModelPortaliQ5U5R9.
SMRiQ5U5R9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini437 – 776HECTPROSITE-ProRule annotationAdd BLAST340

Sequence similaritiesi

Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0941. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00860000133671.
HOGENOMiHOG000208451.
HOVERGENiHBG081596.
InParanoidiQ5U5R9.
KOiK12232.
PhylomeDBiQ5U5R9.
TreeFamiTF315189.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
InterProiIPR000569. HECT_dom.
[Graphical view]
PfamiPF00632. HECT. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5U5R9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEAVRVPSP ATPLVVAAPA PEERKGKESE REKLPPIVSA GAGATAGLDR
60 70 80 90 100
GAKGQISTFS SFISAVSPKK EAAENRSSPA HLVFPNIKNV REPPPICLDV
110 120 130 140 150
RQKQRTSMDA SSSEMKAPVL PEPILPIQPK TVKDFQEDVE KVKSSGDWKA
160 170 180 190 200
VHDFYLTTFD SFPELNAAFK KDATASFNTI EDSGINAKFV NAVYDTLLNT
210 220 230 240 250
PQDVQKTVLK GIINSLLREW KGPRTKDDLR AYFILLQNPQ FNNTSTYVIY
260 270 280 290 300
AHLLRQIATL VEADHHFLVH WFKKLSQKRF KQLVERLLQF ISLRLFPAKP
310 320 330 340 350
EEFPPITKCS WWIPSAAKVL ALLNTANNLV HPPLIPYTDF YNSTLDHIDL
360 370 380 390 400
MEEYHTWQNF GNSHRFSFCQ YPFVISVAAK KIIIQRDSEQ QMINIARQSL
410 420 430 440 450
VDKVSRRQRP DMNILFLNMK VRRTHLVSDS LDELTRKRAD LKKKLKVTFV
460 470 480 490 500
GEAGLDMGGL TKEWFLLLIR QIFHPDYGMF TYHKDSHCHW FSSFKCDNYS
510 520 530 540 550
EFRLVGILMG LAVYNSITLD IRFPPCCYKK LLSPPIIPSD QNIPVGICNV
560 570 580 590 600
TVDDLCQIMP ELAHGLSELL SHEGNVEEDF YSTFQVFQEE FGIIKSYNLK
610 620 630 640 650
PGGDKISVTN QNRKEYVQLY TDFLLNKSIY KQFAAFYYGF HSVCASNALM
660 670 680 690 700
LLRPEEVEIL VCGSPDLDMH ALQRSTQYDG YAKTDLTIKY FWDVVLGFPL
710 720 730 740 750
DLQKKLLHFT TGSDRVPVGG MADLNFKISK NETSTNCLPV AHTCFNQLCL
760 770
PPYKSKKDLK QKLIIGISNS EGFGLE
Length:776
Mass (Da):88,122
Last modified:June 27, 2006 - v2
Checksum:i45B98DD76B33AB1A
GO
Isoform 2 (identifier: Q5U5R9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-207: PQDVQKT → VSIMTCK
     208-776: Missing.

Note: No experimental confirmation available.
Show »
Length:207
Mass (Da):22,360
Checksum:iD4AB053C877247AF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85P → L in AAH40187 (PubMed:15489334).Curated1
Sequence conflicti519L → F in AAH40187 (PubMed:15489334).Curated1
Sequence conflicti540D → G in AAH40187 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02683619P → A.2 PublicationsCorresponds to variant rs7081569dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044965201 – 207PQDVQKT → VSIMTCK in isoform 2. 1 Publication7
Alternative sequenceiVSP_044966208 – 776Missing in isoform 2. 1 PublicationAdd BLAST569

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK094625 mRNA. Translation: BAC04388.1.
AL161798 Genomic DNA. Translation: CAH70050.1.
AL161798, AC023902 Genomic DNA. Translation: CAH70052.1.
AL161798, AC023902 Genomic DNA. Translation: CAH70053.1.
CH471066 Genomic DNA. Translation: EAW50111.1.
BC040187 mRNA. Translation: AAH40187.1.
AL713675 mRNA. Translation: CAD28480.1.
CCDSiCCDS7414.1. [Q5U5R9-1]
CCDS7415.1. [Q5U5R9-2]
RefSeqiNP_001271203.1. NM_001284274.1.
NP_775768.3. NM_173497.3.
NP_877497.3. NM_182765.4.
UniGeneiHs.596096.

Genome annotation databases

EnsembliENST00000298068; ENSP00000298068; ENSG00000165338. [Q5U5R9-1]
ENST00000371681; ENSP00000360746; ENSG00000165338. [Q5U5R9-2]
GeneIDi143279.
KEGGihsa:143279.
UCSCiuc001khk.4. human. [Q5U5R9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK094625 mRNA. Translation: BAC04388.1.
AL161798 Genomic DNA. Translation: CAH70050.1.
AL161798, AC023902 Genomic DNA. Translation: CAH70052.1.
AL161798, AC023902 Genomic DNA. Translation: CAH70053.1.
CH471066 Genomic DNA. Translation: EAW50111.1.
BC040187 mRNA. Translation: AAH40187.1.
AL713675 mRNA. Translation: CAD28480.1.
CCDSiCCDS7414.1. [Q5U5R9-1]
CCDS7415.1. [Q5U5R9-2]
RefSeqiNP_001271203.1. NM_001284274.1.
NP_775768.3. NM_173497.3.
NP_877497.3. NM_182765.4.
UniGeneiHs.596096.

3D structure databases

ProteinModelPortaliQ5U5R9.
SMRiQ5U5R9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126794. 4 interactors.
IntActiQ5U5R9. 1 interactor.
STRINGi9606.ENSP00000298068.

PTM databases

iPTMnetiQ5U5R9.
PhosphoSitePlusiQ5U5R9.

Polymorphism and mutation databases

DMDMi109892196.

Proteomic databases

MaxQBiQ5U5R9.
PaxDbiQ5U5R9.
PeptideAtlasiQ5U5R9.
PRIDEiQ5U5R9.

Protocols and materials databases

DNASUi143279.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298068; ENSP00000298068; ENSG00000165338. [Q5U5R9-1]
ENST00000371681; ENSP00000360746; ENSG00000165338. [Q5U5R9-2]
GeneIDi143279.
KEGGihsa:143279.
UCSCiuc001khk.4. human. [Q5U5R9-1]

Organism-specific databases

CTDi143279.
DisGeNETi143279.
GeneCardsiHECTD2.
HGNCiHGNC:26736. HECTD2.
HPAiHPA037767.
neXtProtiNX_Q5U5R9.
OpenTargetsiENSG00000165338.
PharmGKBiPA134933711.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0941. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00860000133671.
HOGENOMiHOG000208451.
HOVERGENiHBG081596.
InParanoidiQ5U5R9.
KOiK12232.
PhylomeDBiQ5U5R9.
TreeFamiTF315189.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii143279.
PROiQ5U5R9.

Gene expression databases

BgeeiENSG00000165338.
CleanExiHS_HECTD2.
ExpressionAtlasiQ5U5R9. baseline and differential.
GenevisibleiQ5U5R9. HS.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
InterProiIPR000569. HECT_dom.
[Graphical view]
PfamiPF00632. HECT. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHECD2_HUMAN
AccessioniPrimary (citable) accession number: Q5U5R9
Secondary accession number(s): Q5VZ97
, Q5VZ98, Q5VZ99, Q8N1X7, Q8TCP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: November 30, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.