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Q5U5Q9

- UIMC1_MOUSE

UniProt

Q5U5Q9 - UIMC1_MOUSE

Protein

BRCA1-A complex subunit RAP80

Gene

Uimc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    Functioni

    Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1 By similarity.By similarity

    GO - Molecular functioni

    1. histone binding Source: UniProtKB
    2. K63-linked polyubiquitin binding Source: UniProtKB
    3. retinoid X receptor binding Source: MGI

    GO - Biological processi

    1. double-strand break repair Source: UniProtKB
    2. G2 DNA damage checkpoint Source: UniProtKB
    3. histone H2A K63-linked deubiquitination Source: UniProtKB
    4. negative regulation of transcription, DNA-templated Source: HGNC
    5. positive regulation of DNA repair Source: UniProtKB
    6. response to ionizing radiation Source: UniProtKB
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Biological processi

    DNA damage, DNA repair, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    BRCA1-A complex subunit RAP80
    Alternative name(s):
    Receptor-associated protein 80
    Ubiquitin interaction motif-containing protein 1
    Gene namesi
    Name:Uimc1
    Synonyms:Rap80, Rip110, Rxrip110
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:103185. Uimc1.

    Subcellular locationi

    Nucleus By similarity
    Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).By similarity

    GO - Cellular componenti

    1. BRCA1-A complex Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727BRCA1-A complex subunit RAP80PRO_0000097548Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei44 – 441PhosphoserineBy similarity
    Modified residuei46 – 461PhosphoserineBy similarity
    Modified residuei140 – 1401PhosphoserineBy similarity
    Modified residuei205 – 2051PhosphoserineBy similarity
    Modified residuei402 – 4021PhosphoserineBy similarity
    Modified residuei637 – 6371PhosphoserineBy similarity
    Modified residuei665 – 6651PhosphoserineBy similarity
    Modified residuei689 – 6891PhosphoserineBy similarity

    Post-translational modificationi

    Sumoylated.By similarity
    Phosphorylated upon DNA damage by ATM or ATR.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ5U5Q9.
    PRIDEiQ5U5Q9.

    PTM databases

    PhosphoSiteiQ5U5Q9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ5U5Q9.
    BgeeiQ5U5Q9.
    CleanExiMM_UIMC1.
    GenevestigatoriQ5U5Q9.

    Interactioni

    Subunit structurei

    Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1 and UBE2I By similarity. Interacts with NR6A1 and TSP57.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    UbcP629912EBI-7068640,EBI-413074

    Protein-protein interaction databases

    BioGridi203041. 4 interactions.
    IntActiQ5U5Q9. 2 interactions.
    MINTiMINT-4117130.

    Structurei

    Secondary structure

    1
    727
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi81 – 11939

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3A1QX-ray2.20C/F80-120[»]
    ProteinModelPortaliQ5U5Q9.
    SMRiQ5U5Q9. Positions 80-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5U5Q9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati79 – 9618UIM 1Add
    BLAST
    Repeati104 – 12421UIM 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 101101Necessary for transcriptional repressionBy similarityAdd
    BLAST
    Regioni97 – 1037UIM-linker
    Regioni100 – 200101Necessary for interaction with NR6A1 N-terminusBy similarityAdd
    BLAST
    Regioni270 – 400131AIRAdd
    BLAST
    Regioni400 – 508109Necessary for interaction with NR6A1 C-terminusBy similarityAdd
    BLAST
    Regioni513 – 59078Zinc-finger-like regionAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7819LR motifAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi81 – 10828Glu-richAdd
    BLAST

    Domaini

    The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions By similarity.By similarity
    The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.By similarity

    Sequence similaritiesi

    Belongs to the RAP80 family.Curated
    Contains 2 UIM (ubiquitin-interacting motif) repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG72551.
    GeneTreeiENSGT00390000007635.
    HOGENOMiHOG000154772.
    HOVERGENiHBG056783.
    InParanoidiQ8C3Z8.
    PhylomeDBiQ5U5Q9.

    Family and domain databases

    InterProiIPR003903. Ubiquitin-int_motif.
    [Graphical view]
    SMARTiSM00726. UIM. 2 hits.
    [Graphical view]
    PROSITEiPS50330. UIM. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5U5Q9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPRRKKKIKE ASESQNLEKK DLETSSCVSI KKKRRLEDLL IVISDSDGEE    50
    TKEENGLQKT KTKQSNRSKC LAKRKVAHMS EEEQFALALK MSEQEAREVN 100
    NQEEKEEELL RKAIAESLNS CWSSAASATR SRPLAAELSS HSHQENTKDS 150
    GTTEGVWQLV PPSLCKGSHV SQGNEAEQRK EPWDHNENTE EEPVSGSSGS 200
    WDQSSQPVFE NENVKCFDRC TGHLAEHTQC GKPQESTGSG YAFSKAVQGR 250
    GDTSRQCLPI PADTKGLQDT GGTVHYYWGI PFCPAGVDPN QYTNVILCQL 300
    EVYQKSLKMA QRQLVKKRGF GEPVLPRPPF LIQNECGQED QTSDKNEGIS 350
    EDMGDEAKEE RQESRASVWH SETKDFQKSP IKSLKQKLLL EEEPTTSRGQ 400
    SSQGLFVEET SEEGLKSSEG DNSVPTTQSI AALTSKRSLV LMPESSAEEI 450
    TVCPETQLSF LEPLDLNRED SPDSRELPIE VRMAVGDKQV ANREDCMKEN 500
    PPPAVSSSTR VSCPLCNQDF PPTKIEQHAM YCNGLMEQET VLTRRRREAK 550
    NKSDGRTAAQ PALDANRKEK CYLCKSLVPL GEYQCHVEAC LQLAKVDRED 600
    GIEGTRRPRV CAPVEGKQQQ RLKKSKDKGH SQGRLLSLLE QSEHRTTGVE 650
    KKPKYSEVRT FRMPSPEVEE ASCSREMQST LSQLNLNESP IKSFVPVSEA 700
    TNCLVDFKEQ FAFRSRTKSG RGRRRKS 727
    Length:727
    Mass (Da):81,478
    Last modified:May 5, 2009 - v2
    Checksum:iB753BF61180D63ED
    GO
    Isoform 2 (identifier: Q5U5Q9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         401-406: SSQGLF → HCSFDQ
         407-727: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:406
    Mass (Da):45,575
    Checksum:i904F4FDD0F27ECB0
    GO
    Isoform 3 (identifier: Q5U5Q9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         120-400: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:446
    Mass (Da):50,382
    Checksum:i4E76F9B20CF84D80
    GO

    Sequence cautioni

    The sequence AAH37092.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti377 – 3771Q → R in BAC38875. (PubMed:16141072)Curated
    Sequence conflicti450 – 4501I → T in BAC35106. (PubMed:16141072)Curated
    Sequence conflicti450 – 4501I → T in BAC38875. (PubMed:16141072)Curated
    Sequence conflicti450 – 4501I → T in BAE36094. (PubMed:16141072)Curated
    Sequence conflicti554 – 5541D → G in AAH40808. (PubMed:15489334)Curated
    Sequence conflicti722 – 7221G → E in AAC52167. (PubMed:7760852)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei120 – 400281Missing in isoform 3. 1 PublicationVSP_037266Add
    BLAST
    Alternative sequencei401 – 4066SSQGLF → HCSFDQ in isoform 2. 1 PublicationVSP_037267
    Alternative sequencei407 – 727321Missing in isoform 2. 1 PublicationVSP_037268Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK052704 mRNA. Translation: BAC35106.1.
    AK083342 mRNA. Translation: BAC38875.1.
    AK160926 mRNA. Translation: BAE36094.1.
    BC037092 mRNA. Translation: AAH37092.1. Different initiation.
    BC040808 mRNA. Translation: AAH40808.1.
    U22015 mRNA. Translation: AAC52167.1.
    CCDSiCCDS36671.1. [Q5U5Q9-1]
    RefSeqiNP_035437.1. NM_011307.2.
    XP_006517229.1. XM_006517166.1.
    UniGeneiMm.259301.

    Genome annotation databases

    EnsembliENSMUST00000026997; ENSMUSP00000026997; ENSMUSG00000025878.
    ENSMUST00000099496; ENSMUSP00000097095; ENSMUSG00000025878.
    GeneIDi20184.
    KEGGimmu:20184.
    UCSCiuc007qpv.1. mouse. [Q5U5Q9-1]
    uc007qpw.1. mouse. [Q5U5Q9-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK052704 mRNA. Translation: BAC35106.1 .
    AK083342 mRNA. Translation: BAC38875.1 .
    AK160926 mRNA. Translation: BAE36094.1 .
    BC037092 mRNA. Translation: AAH37092.1 . Different initiation.
    BC040808 mRNA. Translation: AAH40808.1 .
    U22015 mRNA. Translation: AAC52167.1 .
    CCDSi CCDS36671.1. [Q5U5Q9-1 ]
    RefSeqi NP_035437.1. NM_011307.2.
    XP_006517229.1. XM_006517166.1.
    UniGenei Mm.259301.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3A1Q X-ray 2.20 C/F 80-120 [» ]
    ProteinModelPortali Q5U5Q9.
    SMRi Q5U5Q9. Positions 80-120.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203041. 4 interactions.
    IntActi Q5U5Q9. 2 interactions.
    MINTi MINT-4117130.

    PTM databases

    PhosphoSitei Q5U5Q9.

    Proteomic databases

    PaxDbi Q5U5Q9.
    PRIDEi Q5U5Q9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026997 ; ENSMUSP00000026997 ; ENSMUSG00000025878 .
    ENSMUST00000099496 ; ENSMUSP00000097095 ; ENSMUSG00000025878 .
    GeneIDi 20184.
    KEGGi mmu:20184.
    UCSCi uc007qpv.1. mouse. [Q5U5Q9-1 ]
    uc007qpw.1. mouse. [Q5U5Q9-3 ]

    Organism-specific databases

    CTDi 51720.
    MGIi MGI:103185. Uimc1.

    Phylogenomic databases

    eggNOGi NOG72551.
    GeneTreei ENSGT00390000007635.
    HOGENOMi HOG000154772.
    HOVERGENi HBG056783.
    InParanoidi Q8C3Z8.
    PhylomeDBi Q5U5Q9.

    Miscellaneous databases

    EvolutionaryTracei Q5U5Q9.
    NextBioi 297717.
    PROi Q5U5Q9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q5U5Q9.
    Bgeei Q5U5Q9.
    CleanExi MM_UIMC1.
    Genevestigatori Q5U5Q9.

    Family and domain databases

    InterProi IPR003903. Ubiquitin-int_motif.
    [Graphical view ]
    SMARTi SM00726. UIM. 2 hits.
    [Graphical view ]
    PROSITEi PS50330. UIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Strain: C57BL/6J.
      Tissue: Extraembryonic tissue, Kidney, Placenta and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and FVB/N.
      Tissue: Mammary cancer and Mammary tumor.
    3. "Isolation of proteins that interact specifically with the retinoid X receptor: two novel orphan receptors."
      Seol W., Choi H.S., Moore D.D.
      Mol. Endocrinol. 9:72-85(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 148-727 (ISOFORM 1), INTERACTION WITH NR6A1.
      Tissue: Liver.
    4. "Tsp57: a novel gene induced during a specific stage of spermatogenesis."
      Kim Y.-S., Nakanishi G., Oudes A.J., Kim K.H., Wang H., Kilpatrick D.L., Jetten A.M.
      Biol. Reprod. 70:106-113(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TSP57.

    Entry informationi

    Entry nameiUIMC1_MOUSE
    AccessioniPrimary (citable) accession number: Q5U5Q9
    Secondary accession number(s): Q3TU77
    , Q60811, Q8C3Z8, Q8C719, Q8K1X7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3