Q5U5Q3 (MEX3C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding protein MEX3C

Alternative name(s):
RING finger and KH domain-containing protein 2
RING finger protein 194

Gene names

Name:	MEX3C
Synonyms:	RKHD2, RNF194
ORF Names:	BM-013

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	659 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding protein. May be involved in post-transcriptional regulatory mechanisms.
Subcellular location	Cytoplasm. Nucleus. Note: Predominantly expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway. Ref.1
Tissue specificity	Highest levels found in fetal brain and testis. Also expressed in thymus, salivary gland and uterus. Week expression in the intestine. Ref.1
Domain	Binds RNA through its KH domains.
Involvement in disease	Note=Genetic variations in MEX3C may be associated with susceptibility to essential hypertension.
Sequence similarities	Contains 2 KH domains. Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAF64269.1 differs from that shown. Reason: Erroneous termination at position 514. Translated as Glu. The sequence AAF64269.1 differs from that shown. Reason: Frameshift at positions 520 and 525.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 659	659	RNA-binding protein MEX3C		PRO_0000278782
Regions
	Domain	232 – 293	62	KH 1
	Domain	326 – 387	62	KH 2
	Zinc finger	608 – 648	41	RING-type
Amino acid modifications
	Modified residue	545	1	Phosphoserine Ref.6
Natural variations
	Natural variant	412	1	T → P. Corresponds to variant rs12970605 [ dbSNP | Ensembl ].		VAR_030832
Experimental info
	Mutagenesis	343	1	G → D: Prevents RNA binding. Ref.1
	Sequence conflict	509	1	I → M in AAF64269. Ref.4
	Sequence conflict	519	1	L → F in AAF64269. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q5U5Q3 [UniParc]. Last modified March 20, 2007. Version 3. Checksum: BAFDB7AD4B331471	FASTA	659	69,366
	10 20 30 40 50 60 MPSGSSAALA LAAAPAPLPQ PPPPPPPPPP PLPPPSGGPE LEGDGLLLRE RLAALGLDDP 70 80 90 100 110 120 SPAEPGAPAL RAPAAAAQGQ ARRAAELSPE ERAPPGRPGA PEAAELELEE DEEEGEEAEL 130 140 150 160 170 180 DGDLLEEEEL EEAEEEDRSS LLLLSPPAAT ASQTQQIPGG SLGSVLLPAA RFDAREAAAA 190 200 210 220 230 240 AAAAGVLYGG DDAQGMMAAM LSHAYGPGGC GAAAAALNGE QAALLRRKSV NTTECVPVPS 250 260 270 280 290 300 SEHVAEIVGR QGCKIKALRA KTNTYIKTPV RGEEPIFVVT GRKEDVAMAK REILSAAEHF 310 320 330 340 350 360 SMIRASRNKN GPALGGLSCS PNLPGQTTVQ VRVPYRVVGL VVGPKGATIK RIQQQTHTYI 370 380 390 400 410 420 VTPSRDKEPV FEVTGMPENV DRAREEIEMH IAMRTGNYIE LNEENDFHYN GTDVSFEGGT 430 440 450 460 470 480 LGSAWLSSNP VPPSRARMIS NYRNDSSSSL GSGSTDSYFG SNRLADFSPT SPFSTGNFWF 490 500 510 520 530 540 GDTLPSVGSE DLAVDSPAFD SLPTSAQTIW TPFEPVNPLS GFGSDPSGNM KTQRRGSQPS 550 560 570 580 590 600 TPRLSPTFPE SIEHPLARRV RSDPPSTGNH VGLPIYIPAF SNGTNSYSSS NGGSTSSSPP 610 620 630 640 650 ESRRKHDCVI CFENEVIAAL VPCGHNLFCM ECANKICEKR TPSCPVCQTA VTQAIQIHS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies." Buchet-Poyau K., Courchet J., Le Hir H., Seraphin B., Scoazec J.-Y., Duret L., Domon-Dell C., Freund J.-N., Billaud M. Nucleic Acids Res. 35:1289-1300(2007) [PubMed: 17267406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-343, PHOSPHORYLATION.
[2]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed: 16177791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 451-659. Tissue: Lung.
[4]	"A novel gene expressed in human bone marrow." Zhao M., Song H., Li N., Peng Y., Han Z., Chen Z. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 508-659. Tissue: Bone marrow.
[5]	"Implication of chromosome 18 in hypertension by sibling pair and association analyses: putative involvement of the RKHD2 gene." Guzman B., Cormand B., Ribases M., Gonzalez-Nunez D., Botey A., Poch E. Hypertension 48:883-891(2006) [PubMed: 17015768] [Abstract] Cited for: POSSIBLE INVOLVEMENT IN SUSCEPTIBILITY TO ESSENTIAL HYPERTENSION.
[6]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-545, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY950679 mRNA. Translation: AAY34147.1. AC090330 Genomic DNA. No translation available. BC041122 mRNA. Translation: AAH41122.1. AF208855 mRNA. Translation: AAF64269.1. Sequence problems.
IPI	IPI00790901.
RefSeq	NP_057710.3. NM_016626.4.
UniGene	Hs.465144.
3D structure databases
ProteinModelPortal	Q5U5Q3.
SMR	Q5U5Q3. Positions 232-396, 606-654.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5U5Q3. 1 interaction.
STRING	Q5U5Q3.
PTM databases
PhosphoSite	Q5U5Q3.
Polymorphism databases
DMDM	134047827.
Proteomic databases
PRIDE	Q5U5Q3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000406189; ENSP00000385610; ENSG00000176624.
GeneID	51320.
KEGG	hsa:51320.
UCSC	uc002lfc.2. human.
Organism-specific databases
CTD	51320.
GeneCards	GC18M048700.
H-InvDB	HIX0014454.
HGNC	HGNC:28040. MEX3C.
HPA	HPA040603.
MIM	611005. gene.
neXtProt	NX_Q5U5Q3.
PharmGKB	PA162395821.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19065.
GeneTree	ENSGT00390000018714.
HOGENOM	HBG505977.
HOVERGEN	HBG071698.
InParanoid	Q5U5Q3.
OMA	SQTIWTP.
OrthoDB	EOG4PK27W.
PhylomeDB	Q5U5Q3.
Gene expression databases
ArrayExpress	Q5U5Q3.
Bgee	Q5U5Q3.
CleanEx	HS_MEX3C.
Genevestigator	Q5U5Q3.
Family and domain databases
InterPro	IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR018957. Znf_C3HC4_RING-type. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
Pfam	PF00013. KH_1. 2 hits. PF00097. zf-C3HC4. 1 hit. [Graphical view]
SMART	SM00322. KH. 2 hits. SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS50084. KH_TYPE_1. 2 hits. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	54697.
SOURCE	Search...

Entry information

Entry name

MEX3C_HUMAN

Accession

Primary (citable) accession number: Q5U5Q3
Secondary accession number(s): A1L022, Q9NZE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	March 20, 2007
Last modified:	January 25, 2012
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.