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Q5U595

- ZRN1A_XENLA

UniProt

Q5U595 - ZRN1A_XENLA

Protein

Ubiquitin thioesterase zranb1-A

Gene

zranb1-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. May play a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei436 – 4361NucleophileBy similarity
    Active sitei578 – 5781Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri79 – 10830RanBP2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri143 – 17331RanBP2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB
    2. ubiquitin thiolesterase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell migration Source: UniProtKB
    2. cytoskeleton organization Source: UniProtKB
    3. positive regulation of Wnt signaling pathway Source: UniProtKB
    4. protein K29-linked deubiquitination Source: UniProtKB
    5. protein K33-linked deubiquitination Source: UniProtKB
    6. protein K63-linked deubiquitination Source: UniProtKB
    7. regulation of cell morphogenesis Source: UniProtKB
    8. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway, Wnt signaling pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC64.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase zranb1-A (EC:3.4.19.12)
    Alternative name(s):
    Zinc finger Ran-binding domain-containing protein 1A
    Gene namesi
    Name:zranb1-a
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-876406. zranb1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 701701Ubiquitin thioesterase zranb1-APRO_0000361556Add
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati253 – 28331ANK 1Add
    BLAST
    Repeati306 – 33328ANK 2Add
    BLAST
    Domaini425 – 585161OTUPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The OTU domain mediates the deubiquitinating activity.By similarity
    The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.By similarity

    Sequence similaritiesi

    Belongs to the peptidase C64 family.Curated
    Contains 2 ANK repeats.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation
    Contains 3 RanBP2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri79 – 10830RanBP2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri143 – 17331RanBP2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    ANK repeat, Repeat, Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG058978.
    KOiK11862.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR001876. Znf_RanBP2.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    PF00641. zf-RanBP. 2 hits.
    [Graphical view]
    SMARTiSM00547. ZnF_RBZ. 3 hits.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    PS01358. ZF_RANBP2_1. 3 hits.
    PS50199. ZF_RANBP2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5U595-1 [UniParc]FASTAAdd to Basket

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    MTEHGIKWGC EYCTYENWPS AIKCTMCRAP RPSGAIITEE PFKNSTPDVG    50
    SMERDIGSPL ICPDSSARPR VKSSYSMEPS SKWSCQICTY LNWPRAIRCT 100
    QCLSQRRTRS PTESPQSSGS GLRSIPSPID PCEEYNDRNK LNIKGQHWTC 150
    SACTYENCAK AKKCVVCDHP TPNNMDAIEL ANTDEASSII NEQDRARWRG 200
    GCSSSNSQRR SPPTSKRDSD MDFQRIELAG AVGSKEEFEL DLKKLKQIKN 250
    RMRKTDWLFL NACVGIVEGD LSAVESYKTS GGDIARQLSA DEVRLLNRPS 300
    AFDVGYTLVH LSIRFQRQDM LAILLTEVSQ HAAKCIPAMV CPELTEQIRR 350
    EIAASVHQRK GDFACYFLTD LVTFTLPADI EDLPPTVQEK LFDEVLDRDV 400
    QKELEEESPI INWSLELGTR LDSRLYALWN RTAGDCLLDS VLQATWGIYD 450
    KDSVLRKALH DSLHDCSHWF YSRWKEWESW YSQSFGLHFS LREEQWQEDW 500
    AFILSLASQP GASLEQTHIF VLAHILRRPI IVYGVKYYKS FRGETLGYTR 550
    FQGVYLPLLW EQSFCWKSPI ALGYTRGHFS ALVAMENDGF DNRGAGANLN 600
    TDDDITVTFL PLVDSERKLL HIHFLSAQEL GNEDQQEKLL REWMDCCVTE 650
    GGVLVAMQKS SRRRNHPLVT QMVEKWLDGY RQIRPCTALS DGEEDEDDED 700
    E 701
    Length:701
    Mass (Da):79,972
    Last modified:December 7, 2004 - v1
    Checksum:i4AF668558912CF1D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC084789 mRNA. Translation: AAH84789.1.
    RefSeqiNP_001088463.1. NM_001094994.1.
    UniGeneiXl.25418.

    Genome annotation databases

    GeneIDi495328.
    KEGGixla:495328.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC084789 mRNA. Translation: AAH84789.1 .
    RefSeqi NP_001088463.1. NM_001094994.1.
    UniGenei Xl.25418.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C64.004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 495328.
    KEGGi xla:495328.

    Organism-specific databases

    CTDi 495328.
    Xenbasei XB-GENE-876406. zranb1.

    Phylogenomic databases

    HOVERGENi HBG058978.
    KOi K11862.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR001876. Znf_RanBP2.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    PF00641. zf-RanBP. 2 hits.
    [Graphical view ]
    SMARTi SM00547. ZnF_RBZ. 3 hits.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    PS01358. ZF_RANBP2_1. 3 hits.
    PS50199. ZF_RANBP2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.

    Entry informationi

    Entry nameiZRN1A_XENLA
    AccessioniPrimary (citable) accession number: Q5U595
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3