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Protein

Ribosomal RNA-processing protein 8

Gene

Rrp8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei282S-adenosyl-L-methionineBy similarity1
Binding sitei317S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei335S-adenosyl-L-methionineBy similarity1
Binding sitei347S-adenosyl-L-methionineBy similarity1
Binding sitei348S-adenosyl-L-methionineBy similarity1
Binding sitei364S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Methyltransferase, Repressor, Transferase
Biological processrRNA processing, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-RNO-427359 SIRT1 negatively regulates rRNA expression

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA-processing protein 8 (EC:2.1.1.-)
Alternative name(s):
Cerebral protein 1 homolog
Gene namesi
Name:Rrp8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1308302 Rrp8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000840921 – 457Ribosomal RNA-processing protein 8Add BLAST457

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62PhosphoserineCombined sources1
Modified residuei64PhosphoserineCombined sources1
Modified residuei105PhosphoserineBy similarity1
Modified residuei172PhosphoserineBy similarity1
Modified residuei177PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5U4F0
PRIDEiQ5U4F0

PTM databases

iPTMnetiQ5U4F0
PhosphoSitePlusiQ5U4F0

Expressioni

Gene expression databases

BgeeiENSRNOG00000018766
ExpressionAtlasiQ5U4F0 baseline and differential
GenevisibleiQ5U4F0 RN

Interactioni

Subunit structurei

Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025651

Structurei

3D structure databases

ProteinModelPortaliQ5U4F0
SMRiQ5U4F0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi65 – 69Poly-Glu5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3045 Eukaryota
ENOG4111J8Z LUCA
GeneTreeiENSGT00390000006189
HOGENOMiHOG000164004
InParanoidiQ5U4F0
KOiK14850
OMAiLMGTNIR
OrthoDBiEOG091G0K2R
PhylomeDBiQ5U4F0
TreeFamiTF313749

Family and domain databases

InterProiView protein in InterPro
IPR007823 RRP8
IPR029063 SAM-dependent_MTases
IPR023576 UbiE/COQ5_MeTrFase_CS
PANTHERiPTHR12787 PTHR12787, 1 hit
PfamiView protein in Pfam
PF05148 Methyltransf_8, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Q5U4F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEEPEWVEA APAIVGLRPV TTQVQAATAP PVKGRKRRHL LATLRALEAA
60 70 80 90 100
SLSQQCPSLP GSDSEEEEEV GRKKRHFQRS SLANVSKEVG KKRKGKCQKQ
110 120 130 140 150
APFISDSEGK EVERTCHRQA PPLGGISAGE EKGKRKCQEY SYLHPTQSLN
160 170 180 190 200
SVDQTVHNSR TSTATLDPPK SSRESASPNS SHTLSRKQWR NRQKNKRRHK
210 220 230 240 250
NKFRPLETQD QVPLKASIEE TEVPPAPKSD SQETRAGALR ARMTQRLDGA
260 270 280 290 300
RFRYLNEQLY SGPSSAAQCL FQEDPEAFLL YHRGFQRQVK KWPLHPVDRI
310 320 330 340 350
AKDLRQKPAS LVVADFGCGD CRLASSVRNP VHCFDLAALD PRVTVCDMAQ
360 370 380 390 400
VPLEDESVDV AVFCLSLMGT NIRDFLEEAN RVLKPGGLLK VAEVSSRFED
410 420 430 440 450
IRTFLGAVTK LGFKVIYKDL TNSHFFLFDF EKTGPPRVGP KAQLSGLKLQ

PCLYKHR
Length:457
Mass (Da):51,216
Last modified:December 7, 2004 - v1
Checksum:i37B2AF0216D48F6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085119 mRNA Translation: AAH85119.1
RefSeqiNP_001008347.1, NM_001008346.1
UniGeneiRn.14828

Genome annotation databases

EnsembliENSRNOT00000025651; ENSRNOP00000025651; ENSRNOG00000018766
GeneIDi308911
KEGGirno:308911
UCSCiRGD:1308302 rat

Similar proteinsi

Entry informationi

Entry nameiRRP8_RAT
AccessioniPrimary (citable) accession number: Q5U4F0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 7, 2004
Last modified: April 25, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome