Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosomal RNA-processing protein 8

Gene

Rrp8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei282 – 2821S-adenosyl-L-methionineBy similarity
Binding sitei317 – 3171S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei335 – 3351S-adenosyl-L-methionineBy similarity
Binding sitei347 – 3471S-adenosyl-L-methionineBy similarity
Binding sitei348 – 3481S-adenosyl-L-methionineBy similarity
Binding sitei364 – 3641S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Repressor, Transferase

Keywords - Biological processi

rRNA processing, Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-RNO-427359. SIRT1 negatively regulates rRNA Expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA-processing protein 8 (EC:2.1.1.-)
Alternative name(s):
Cerebral protein 1 homolog
Gene namesi
Name:Rrp8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1308302. Rrp8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Ribosomal RNA-processing protein 8PRO_0000084092Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphoserineCombined sources
Modified residuei64 – 641PhosphoserineCombined sources
Modified residuei105 – 1051PhosphoserineBy similarity
Modified residuei172 – 1721PhosphoserineBy similarity
Modified residuei177 – 1771PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5U4F0.
PRIDEiQ5U4F0.

PTM databases

iPTMnetiQ5U4F0.
PhosphoSiteiQ5U4F0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018766.
ExpressionAtlasiQ5U4F0. baseline and differential.
GenevisibleiQ5U4F0. RN.

Interactioni

Subunit structurei

Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025651.

Structurei

3D structure databases

ProteinModelPortaliQ5U4F0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi65 – 695Poly-Glu

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3045. Eukaryota.
ENOG4111J8Z. LUCA.
GeneTreeiENSGT00390000006189.
HOGENOMiHOG000164004.
InParanoidiQ5U4F0.
KOiK14850.
OMAiLMGTNIR.
OrthoDBiEOG091G0K2R.
PhylomeDBiQ5U4F0.
TreeFamiTF313749.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007823. RRP8.
IPR029063. SAM-dependent_MTases.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view]
PANTHERiPTHR12787. PTHR12787. 1 hit.
PfamiPF05148. Methyltransf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5U4F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEEPEWVEA APAIVGLRPV TTQVQAATAP PVKGRKRRHL LATLRALEAA
60 70 80 90 100
SLSQQCPSLP GSDSEEEEEV GRKKRHFQRS SLANVSKEVG KKRKGKCQKQ
110 120 130 140 150
APFISDSEGK EVERTCHRQA PPLGGISAGE EKGKRKCQEY SYLHPTQSLN
160 170 180 190 200
SVDQTVHNSR TSTATLDPPK SSRESASPNS SHTLSRKQWR NRQKNKRRHK
210 220 230 240 250
NKFRPLETQD QVPLKASIEE TEVPPAPKSD SQETRAGALR ARMTQRLDGA
260 270 280 290 300
RFRYLNEQLY SGPSSAAQCL FQEDPEAFLL YHRGFQRQVK KWPLHPVDRI
310 320 330 340 350
AKDLRQKPAS LVVADFGCGD CRLASSVRNP VHCFDLAALD PRVTVCDMAQ
360 370 380 390 400
VPLEDESVDV AVFCLSLMGT NIRDFLEEAN RVLKPGGLLK VAEVSSRFED
410 420 430 440 450
IRTFLGAVTK LGFKVIYKDL TNSHFFLFDF EKTGPPRVGP KAQLSGLKLQ

PCLYKHR
Length:457
Mass (Da):51,216
Last modified:December 7, 2004 - v1
Checksum:i37B2AF0216D48F6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085119 mRNA. Translation: AAH85119.1.
RefSeqiNP_001008347.1. NM_001008346.1.
UniGeneiRn.14828.

Genome annotation databases

EnsembliENSRNOT00000025651; ENSRNOP00000025651; ENSRNOG00000018766.
GeneIDi308911.
KEGGirno:308911.
UCSCiRGD:1308302. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085119 mRNA. Translation: AAH85119.1.
RefSeqiNP_001008347.1. NM_001008346.1.
UniGeneiRn.14828.

3D structure databases

ProteinModelPortaliQ5U4F0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025651.

PTM databases

iPTMnetiQ5U4F0.
PhosphoSiteiQ5U4F0.

Proteomic databases

PaxDbiQ5U4F0.
PRIDEiQ5U4F0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025651; ENSRNOP00000025651; ENSRNOG00000018766.
GeneIDi308911.
KEGGirno:308911.
UCSCiRGD:1308302. rat.

Organism-specific databases

CTDi23378.
RGDi1308302. Rrp8.

Phylogenomic databases

eggNOGiKOG3045. Eukaryota.
ENOG4111J8Z. LUCA.
GeneTreeiENSGT00390000006189.
HOGENOMiHOG000164004.
InParanoidiQ5U4F0.
KOiK14850.
OMAiLMGTNIR.
OrthoDBiEOG091G0K2R.
PhylomeDBiQ5U4F0.
TreeFamiTF313749.

Enzyme and pathway databases

ReactomeiR-RNO-427359. SIRT1 negatively regulates rRNA Expression.

Miscellaneous databases

PROiQ5U4F0.

Gene expression databases

BgeeiENSRNOG00000018766.
ExpressionAtlasiQ5U4F0. baseline and differential.
GenevisibleiQ5U4F0. RN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007823. RRP8.
IPR029063. SAM-dependent_MTases.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view]
PANTHERiPTHR12787. PTHR12787. 1 hit.
PfamiPF05148. Methyltransf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRP8_RAT
AccessioniPrimary (citable) accession number: Q5U4F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 7, 2004
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.