Reviewed, UniProtKB/Swiss-Prot Q5U4E8 (ANM7_RAT)

Names and origin

Protein names

Recommended name:
    Protein arginine N-methyltransferase 7
    EC=2.1.1.-
Alternative name(s):
    Histone-arginine N-methyltransferase PRMT7
    EC=2.1.1.125
    [Myelin basic protein]-arginine N-methyltransferase PRMT7
    EC=2.1.1.126

Gene names

Name:

Prmt7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3sme2. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3sme2, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo By similarity.
Catalytic activity	S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone]. S-adenosyl-L-methionine + [myelin basic protein]-arginine = S-adenosyl-L-homocysteine + [myelin basic protein]-N(omega)-methyl-arginine.
Subunit structure	Homodimer and heterodimer. Interacts with CTCFL, PRMT5 and SNRPD3 By similarity.
Subcellular location	Cytoplasm › cytosol By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family. PRMT7 subfamily.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation during gametogenesis Inferred from sequence or structural similarity. Source: UniProtKB cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW histone arginine methylation Inferred from sequence or structural similarity. Source: UniProtKB peptidyl-arginine methylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of gene expression by genetic imprinting Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW spliceosomal snRNP assembly Inferred from sequence or structural similarity. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	[myelin basic protein]-arginine N-methyltransferase activity Inferred from electronic annotation. Source: EC histone methyltransferase activity (H4-R3 specific) Inferred from sequence or structural similarity. Source: UniProtKB protein-arginine omega-N symmetric methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 693	693	Protein arginine N-methyltransferase 7		PRO_0000373902

Sequences

	Sequence		Length	Mass (Da)
	Q5U4E8-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 757BB76971EFDA48	FASTA	693	78,346
	10 20 30 40 50 60 MKVFCGRANP TTGSLEWLEE DEHYDYHQEI ARSSYADMLH DKDRNIKYYQ GIRAAVSRVK 70 80 90 100 110 120 DKGQKALVLD IGTGTGLLSM MAVTAGADFC YAVEVFKPMA EAAVKIVEKN GFSDKIKVIN 130 140 150 160 170 180 KHSTEVTVGP DGDLPCRANI LVTELFDTEL IGEGALPSYE HAHKHLVQED CEAVPHRATV 190 200 210 220 230 240 YAQLVESKRM WSWNKLFPVR VQTGLGEQLI IPPSELERCP GAPSVYDIQL NQVSPADFTV 250 260 270 280 290 300 LSDVLPMFSV DFSKQVSSSA ACHSKQFVPL ASGQAQVVLS WWDIEMDPEG KIKCTMAPFW 310 320 330 340 350 360 AQTDPQELQW RDHWMQCVYF LPQEEPIMQG SPRCLAAHHD DYCVWYSLQR TSPDENNSAY 370 380 390 400 410 420 QVRPVCDCQA HLLWNRPRFG EINDQDRTDH YARALRTMLM PGSICLCVSD GSLLSVLAHH 430 440 450 460 470 480 LGAEQVFTVE SSVASYRLMK RIFKVNHLED KITVINKRPE LLTSADLEGK KVSLLLGEPF 490 500 510 520 530 540 FTTSLLPWHN LYFWYVRTSV DQHLAPGAVV MPQAASLHAV IVEFRDLWRI RSPCGDCEGF 550 560 570 580 590 600 DVHIMDDMIK HSLDFRESRE AEPQPLWEYP CRSLSEPRQI LTFDFQQPIP QQPMQSRGVM 610 620 630 640 650 660 ELRRPGKSHG AVLWMEYQLT PDSTVSTGLM NPAEDKGDCC WNPHCKQAVY FLSATLDPSA 670 680 690 PLDGPQSVSY AVEFHPLTGD ITMEFRLADD TLN

References

[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH473972 Genomic DNA. Translation: EDL92443.1. BC085121 mRNA. Translation: AAH85121.1.
IPI	IPI00365189.
RefSeq	NP_001014175.1.
UniGene	Rn.14954
3D structure databases
SMR	Q5U4E8. Positions 23-350.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5U4E8.
Genome annotation databases
Ensembl	ENSRNOT00000000275; ENSRNOP00000000275; ENSRNOG00000000258; Rattus norvegicus. [Genome view]
GeneID	361402.
KEGG	rno:361402.
NMPDR	fig|10116.3.peg.14939.
UCSC	NM_001014153. rat.
Organism-specific databases
CTD	361402.
RGD	1304869. Prmt7.
Phylogenomic databases
eggNOG	roNOG09446.
HOVERGEN	Q5U4E8.
InParanoid	Q5U4E8.
OMA	YDIQLNQ.
OrthoDB	EOG9K0T74.
PhylomeDB	Q5U4E8.
Gene expression databases
ArrayExpress	Q5U4E8.
Genevestigator	Q5U4E8.
Family and domain databases
InterPro	IPR014644. Arg_N-MeTrfase. IPR010456. PrmA_MeTrfase. [Graphical view]
Pfam	PF06325. PrmA. 1 hit. [Graphical view]
PIRSF	PIRSF036946. Arg_N-mtase. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	676175.

Entry information

Entry name

ANM7_RAT

Accession

Primary (citable) accession number: Q5U4E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	December 7, 2004
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)