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Protein

THO complex subunit 6 homolog

Gene

Thoc6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway.Plays a role in apoptosis negative control involved in brain development (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Apoptosis, mRNA processing, mRNA splicing, mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
THO complex subunit 6 homolog
Alternative name(s):
WD repeat-containing protein 58
Gene namesi
Name:Thoc6
Synonyms:Wdr58
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2677480. Thoc6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341THO complex subunit 6 homologPRO_0000233159Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei180 – 1801PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5U4D9.
MaxQBiQ5U4D9.
PaxDbiQ5U4D9.
PeptideAtlasiQ5U4D9.
PRIDEiQ5U4D9.

2D gel databases

REPRODUCTION-2DPAGEIPI00123949.

PTM databases

PhosphoSiteiQ5U4D9.

Expressioni

Gene expression databases

BgeeiQ5U4D9.
CleanExiMM_THOC6.
ExpressionAtlasiQ5U4D9. baseline and differential.
GenevisibleiQ5U4D9. MM.

Interactioni

Subunit structurei

Component of the THO complex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7; together with at least ALYREF/THOC4, DDX39B, SARNP/CIP29 and CHTOP, THO forms the transcription/export (TREX) complex which seems to have a dynamic structure involving ATP-dependent remodeling.By similarity

Protein-protein interaction databases

BioGridi239749. 1 interaction.
IntActiQ5U4D9. 1 interaction.
STRINGi10090.ENSMUSP00000038137.

Structurei

3D structure databases

ProteinModelPortaliQ5U4D9.
SMRiQ5U4D9. Positions 22-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati22 – 6140WD 1Add
BLAST
Repeati74 – 11239WD 2Add
BLAST
Repeati124 – 16542WD 3Add
BLAST
Repeati166 – 20540WD 4Add
BLAST
Repeati215 – 25440WD 5Add
BLAST
Repeati256 – 29338WD 6Add
BLAST
Repeati295 – 33945WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat THOC6 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0649. Eukaryota.
ENOG410XTCD. LUCA.
GeneTreeiENSGT00390000015278.
HOGENOMiHOG000008085.
HOVERGENiHBG079643.
InParanoidiQ5U4D9.
KOiK13175.
OMAiGRCVNQW.
OrthoDBiEOG7Z69CM.
PhylomeDBiQ5U4D9.
TreeFamiTF324760.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U4D9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHAAPLAVP LGQAEVFQAL QRLHMTIFSQ SVSPCGKFLA AGNNYGQIAI
60 70 80 90 100
FSLSAALSSE AKEESKKPVV VFHAHDGPVY SMVSTDRHLL SAGDGEVKGW
110 120 130 140 150
LWAEILKKGC KELWRRQPPY RTSLEVPEIN ALLLVPKENS LILAGGDCQL
160 170 180 190 200
HSMDLETGAF TRALRGHTDY IHCLALRERS PEVLSGGEDG AVRLWDLRIA
210 220 230 240 250
KEVQTIEVYK HEECSRPHNG RWIGCLATDS DWMVCGGGPA LTLWHLRSST
260 270 280 290 300
PTTVFPIRAP QKHVTFYQDL ILSAGQGCCV NHWQLSGELK AQVPGSSPGL
310 320 330 340
LSLSLNQQPA APECKVLTAS GNSCRVDVFT NLGYRAFSLS F
Length:341
Mass (Da):37,315
Last modified:December 7, 2004 - v1
Checksum:iD5B32EC62A7E6D40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085131 mRNA. Translation: AAH85131.1.
BC089551 mRNA. Translation: AAH89551.1.
CCDSiCCDS28454.1.
RefSeqiNP_001008425.1. NM_001008425.1.
XP_006524607.1. XM_006524544.2.
UniGeneiMm.328831.

Genome annotation databases

EnsembliENSMUST00000047436; ENSMUSP00000038137; ENSMUSG00000041319.
ENSMUST00000095579; ENSMUSP00000093239; ENSMUSG00000041319.
GeneIDi386612.
KEGGimmu:386612.
UCSCiuc008ass.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085131 mRNA. Translation: AAH85131.1.
BC089551 mRNA. Translation: AAH89551.1.
CCDSiCCDS28454.1.
RefSeqiNP_001008425.1. NM_001008425.1.
XP_006524607.1. XM_006524544.2.
UniGeneiMm.328831.

3D structure databases

ProteinModelPortaliQ5U4D9.
SMRiQ5U4D9. Positions 22-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi239749. 1 interaction.
IntActiQ5U4D9. 1 interaction.
STRINGi10090.ENSMUSP00000038137.

PTM databases

PhosphoSiteiQ5U4D9.

2D gel databases

REPRODUCTION-2DPAGEIPI00123949.

Proteomic databases

EPDiQ5U4D9.
MaxQBiQ5U4D9.
PaxDbiQ5U4D9.
PeptideAtlasiQ5U4D9.
PRIDEiQ5U4D9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047436; ENSMUSP00000038137; ENSMUSG00000041319.
ENSMUST00000095579; ENSMUSP00000093239; ENSMUSG00000041319.
GeneIDi386612.
KEGGimmu:386612.
UCSCiuc008ass.1. mouse.

Organism-specific databases

CTDi79228.
MGIiMGI:2677480. Thoc6.

Phylogenomic databases

eggNOGiKOG0649. Eukaryota.
ENOG410XTCD. LUCA.
GeneTreeiENSGT00390000015278.
HOGENOMiHOG000008085.
HOVERGENiHBG079643.
InParanoidiQ5U4D9.
KOiK13175.
OMAiGRCVNQW.
OrthoDBiEOG7Z69CM.
PhylomeDBiQ5U4D9.
TreeFamiTF324760.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72187. mRNA 3'-end processing.

Miscellaneous databases

PROiQ5U4D9.
SOURCEiSearch...

Gene expression databases

BgeeiQ5U4D9.
CleanExiMM_THOC6.
ExpressionAtlasiQ5U4D9. baseline and differential.
GenevisibleiQ5U4D9. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Fetal brain and Kidney.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiTHOC6_MOUSE
AccessioniPrimary (citable) accession number: Q5U4D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: December 7, 2004
Last modified: July 6, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.